Human internal mammary artery (IMA) transplantation and stenting: a human model to study the development of in-stent restenosis
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Human internal mammary artery (IMA) transplantation and stenting: a human model to study the development of in-stent restenosis. / Hua, Xiaoqin; Deuse, Tobias; Michelakis, Evangelos D; Haromy, Alois; Tsao, Phil S; Maegdefessel, Lars; Erben, Reinhold G; Bergow, Claudia; Behnisch, Boris B; Reichenspurner, Hermann; Robbins, Robert C; Schrepfer, Sonja.
in: JOVE-J VIS EXP, Nr. 63, 63, 09.05.2012, S. e3663.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Human internal mammary artery (IMA) transplantation and stenting: a human model to study the development of in-stent restenosis
AU - Hua, Xiaoqin
AU - Deuse, Tobias
AU - Michelakis, Evangelos D
AU - Haromy, Alois
AU - Tsao, Phil S
AU - Maegdefessel, Lars
AU - Erben, Reinhold G
AU - Bergow, Claudia
AU - Behnisch, Boris B
AU - Reichenspurner, Hermann
AU - Robbins, Robert C
AU - Schrepfer, Sonja
PY - 2012/5/9
Y1 - 2012/5/9
N2 - Preclinical in vivo research models to investigate pathobiological and pathophysiological processes in the development of intimal hyperplasia after vessel stenting are crucial for translational approaches (1,2). The commonly used animal models include mice, rats, rabbits, and pigs (3-5). However, the translation of these models into clinical settings remains difficult, since those biological processes are already studied in animal vessels but never performed before in human research models (6,7). In this video we demonstrate a new humanized model to overcome this translational gap. The shown procedure is reproducible, easy, and fast to perform and is suitable to study the development of intimal hyperplasia and the applicability of diverse stents. This video shows how to perform the stent technique in human vessels followed by transplantation into immunodeficient rats, and identifies the origin of proliferating cells as human.
AB - Preclinical in vivo research models to investigate pathobiological and pathophysiological processes in the development of intimal hyperplasia after vessel stenting are crucial for translational approaches (1,2). The commonly used animal models include mice, rats, rabbits, and pigs (3-5). However, the translation of these models into clinical settings remains difficult, since those biological processes are already studied in animal vessels but never performed before in human research models (6,7). In this video we demonstrate a new humanized model to overcome this translational gap. The shown procedure is reproducible, easy, and fast to perform and is suitable to study the development of intimal hyperplasia and the applicability of diverse stents. This video shows how to perform the stent technique in human vessels followed by transplantation into immunodeficient rats, and identifies the origin of proliferating cells as human.
KW - Animals
KW - Cell Growth Processes/physiology
KW - Graft Occlusion, Vascular/etiology
KW - Humans
KW - Mammary Arteries/cytology
KW - Rats
KW - Rats, Nude
KW - Stents
KW - Transplantation, Heterologous
KW - Vascular Grafting/methods
U2 - 10.3791/3663
DO - 10.3791/3663
M3 - SCORING: Journal article
C2 - 22617624
SP - e3663
JO - JOVE-J VIS EXP
JF - JOVE-J VIS EXP
SN - 1940-087X
IS - 63
M1 - 63
ER -