Human formyl peptide receptor 1 (FPR1) c.32C>T SNP is associated with decreased soluble E-selectin levels
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Human formyl peptide receptor 1 (FPR1) c.32C>T SNP is associated with decreased soluble E-selectin levels. / Benachour, Hamanou; Zaiou, Mohamed; Herbeth, Bernard; Lambert, Daniel; Lamont, John V; Pfister, Michèle; Siest, Gérard; Tiret, Laurence; Blankenberg, Stefan; Fitzgerald, Peter S; Visvikis-Siest, Sophie.
in: PHARMACOGENOMICS, Jahrgang 10, Nr. 6, 06.2009, S. 951-959.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Human formyl peptide receptor 1 (FPR1) c.32C>T SNP is associated with decreased soluble E-selectin levels
AU - Benachour, Hamanou
AU - Zaiou, Mohamed
AU - Herbeth, Bernard
AU - Lambert, Daniel
AU - Lamont, John V
AU - Pfister, Michèle
AU - Siest, Gérard
AU - Tiret, Laurence
AU - Blankenberg, Stefan
AU - Fitzgerald, Peter S
AU - Visvikis-Siest, Sophie
PY - 2009/6
Y1 - 2009/6
N2 - AIMS: The human formyl peptide receptor (FPR) is a G protein-coupled chemoattractant receptor that is thought to mediate inflammatory responses. The FPR1 gene is highly polymorphic. In a recent study, the FPR1 c.32C>T SNP, resulting in the amino-acid substitution I11T, was reported to be significantly associated with C-reactive protein levels. Therefore, this study sought to determine if the impact of such a genetic variation extends to other clinical parameters associated with inflammation, including cytokines, adhesion molecules and inflammatory markers.MATERIALS & METHODS: This study was carried out on a subsample of 325 adults selected from the STANISLAS cohort study. The FPR1 c.32C>T SNP was genotyped using PCR amplification followed by restriction enzyme digestion. Anthropometric measurements and biochemical profiles were assessed for each individual.RESULTS: The allele frequencies of FPR1 c.32C>T were 0.74 for the 32C allele and 0.26 for the 32T allele. Genotype frequencies were 0.55 for C/C, 0.38 for C/T and 0.07 for T/T. After adjusting for age, sex, BMI, alcohol and cigarette consumption, oral contraceptive, antibiotics and anti-inflammatory drug use, statistical analysis (under a recessive model of inheritance) demonstrated that serum E-selectin levels were 68% lower in individuals homozygous for T/T than in those with C/T or C/C genotypes (p = 0.001). However, no significant correlations were found for C-reactive protein or the other 18 tested clinical parameters that were analyzed in this study.CONCLUSION: The FPR1 c.32C>T SNP may be associated with E-selectin levels in the French population. Although of importance, these findings need confirmation in larger studies.
AB - AIMS: The human formyl peptide receptor (FPR) is a G protein-coupled chemoattractant receptor that is thought to mediate inflammatory responses. The FPR1 gene is highly polymorphic. In a recent study, the FPR1 c.32C>T SNP, resulting in the amino-acid substitution I11T, was reported to be significantly associated with C-reactive protein levels. Therefore, this study sought to determine if the impact of such a genetic variation extends to other clinical parameters associated with inflammation, including cytokines, adhesion molecules and inflammatory markers.MATERIALS & METHODS: This study was carried out on a subsample of 325 adults selected from the STANISLAS cohort study. The FPR1 c.32C>T SNP was genotyped using PCR amplification followed by restriction enzyme digestion. Anthropometric measurements and biochemical profiles were assessed for each individual.RESULTS: The allele frequencies of FPR1 c.32C>T were 0.74 for the 32C allele and 0.26 for the 32T allele. Genotype frequencies were 0.55 for C/C, 0.38 for C/T and 0.07 for T/T. After adjusting for age, sex, BMI, alcohol and cigarette consumption, oral contraceptive, antibiotics and anti-inflammatory drug use, statistical analysis (under a recessive model of inheritance) demonstrated that serum E-selectin levels were 68% lower in individuals homozygous for T/T than in those with C/T or C/C genotypes (p = 0.001). However, no significant correlations were found for C-reactive protein or the other 18 tested clinical parameters that were analyzed in this study.CONCLUSION: The FPR1 c.32C>T SNP may be associated with E-selectin levels in the French population. Although of importance, these findings need confirmation in larger studies.
KW - Adult
KW - Alleles
KW - Anthropometry
KW - Biomarkers/blood
KW - Cohort Studies
KW - E-Selectin/blood
KW - Female
KW - France
KW - Gene Frequency
KW - Genes, Recessive
KW - Genetic Predisposition to Disease
KW - Genotype
KW - Homozygote
KW - Humans
KW - Inflammation/etiology
KW - Male
KW - Middle Aged
KW - Models, Genetic
KW - Polymorphism, Single Nucleotide
KW - Receptors, Formyl Peptide/genetics
KW - Solubility
U2 - 10.2217/pgs.09.29
DO - 10.2217/pgs.09.29
M3 - SCORING: Journal article
C2 - 19530962
VL - 10
SP - 951
EP - 959
JO - PHARMACOGENOMICS
JF - PHARMACOGENOMICS
SN - 1462-2416
IS - 6
ER -