HPV-negative and HPV-positive HNSCC cell lines show similar numerical but different structural chromosomal aberrations
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HPV-negative and HPV-positive HNSCC cell lines show similar numerical but different structural chromosomal aberrations. / Arenz, Andrea; Patze, Johannes; Kornmann, Evelyn; Wilhelm, Jochen; Ziemann, Frank; Wagner, Steffen; Wittig, Andrea; Schoetz, Ulrike; Engenhart-Cabillic, Rita; Dikomey, Ekkehard; Fritz, Barbara.
in: HEAD NECK-J SCI SPEC, Jahrgang 41, Nr. 11, 11.2019, S. 3869-3879.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - HPV-negative and HPV-positive HNSCC cell lines show similar numerical but different structural chromosomal aberrations
AU - Arenz, Andrea
AU - Patze, Johannes
AU - Kornmann, Evelyn
AU - Wilhelm, Jochen
AU - Ziemann, Frank
AU - Wagner, Steffen
AU - Wittig, Andrea
AU - Schoetz, Ulrike
AU - Engenhart-Cabillic, Rita
AU - Dikomey, Ekkehard
AU - Fritz, Barbara
N1 - © 2019 The Authors. Head & Neck published by Wiley Periodicals, Inc.
PY - 2019/11
Y1 - 2019/11
N2 - BACKGROUND: It was tested whether the difference in carcinogenesis between noxa and human papillomavirus (HPV)-driven head and neck squamous cell carcinoma (HNSCC) is associated with a variation in genomic instability.METHODS: Conventional and molecular cytogenetics in HPV-positive and HPV-negative HNSCC cell lines.RESULTS: Numerical aneuploidy determined by multicolor fluorescence in situ hybridization and DNA ploidy was very similar for both entities with most chromosomes being present either in quadruplicate or triplicate, and only few are still diploid with, however, a striking similarity in the overall pattern. A clear difference was seen concerning the translocations formed, with no difference in the total amount but with a significantly higher genomic instability of HPV-positive cell lines at chromosome 3 as compared to HPV-negative cells.CONCLUSION: The different processes of carcinogenesis of HPV-positive and HPV-negative HNSCC appear to result in a similar pattern of numerical but a clear difference in structural chromosomal aberrations.
AB - BACKGROUND: It was tested whether the difference in carcinogenesis between noxa and human papillomavirus (HPV)-driven head and neck squamous cell carcinoma (HNSCC) is associated with a variation in genomic instability.METHODS: Conventional and molecular cytogenetics in HPV-positive and HPV-negative HNSCC cell lines.RESULTS: Numerical aneuploidy determined by multicolor fluorescence in situ hybridization and DNA ploidy was very similar for both entities with most chromosomes being present either in quadruplicate or triplicate, and only few are still diploid with, however, a striking similarity in the overall pattern. A clear difference was seen concerning the translocations formed, with no difference in the total amount but with a significantly higher genomic instability of HPV-positive cell lines at chromosome 3 as compared to HPV-negative cells.CONCLUSION: The different processes of carcinogenesis of HPV-positive and HPV-negative HNSCC appear to result in a similar pattern of numerical but a clear difference in structural chromosomal aberrations.
U2 - 10.1002/hed.25924
DO - 10.1002/hed.25924
M3 - SCORING: Journal article
C2 - 31441163
VL - 41
SP - 3869
EP - 3879
JO - HEAD NECK-J SCI SPEC
JF - HEAD NECK-J SCI SPEC
SN - 1043-3074
IS - 11
ER -