Glucocorticoid receptors α and β are modulated sex specifically in human placentas of intrauterine growth restriction (IUGR)
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Glucocorticoid receptors α and β are modulated sex specifically in human placentas of intrauterine growth restriction (IUGR). / Hutter, Stefan; Hepp, Paula; Hofmann, Simone; Kuhn, Christina; Messner, Julia; Andergassen, Ulrich; Mayr, Doris; Emilia Solano, Maria; Obermeier, Viola; Mahner, Sven; Arck, Petra; Jeschke, Udo.
in: ARCH GYNECOL OBSTET, Jahrgang 300, Nr. 2, 08.2019, S. 323-335.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Glucocorticoid receptors α and β are modulated sex specifically in human placentas of intrauterine growth restriction (IUGR)
AU - Hutter, Stefan
AU - Hepp, Paula
AU - Hofmann, Simone
AU - Kuhn, Christina
AU - Messner, Julia
AU - Andergassen, Ulrich
AU - Mayr, Doris
AU - Emilia Solano, Maria
AU - Obermeier, Viola
AU - Mahner, Sven
AU - Arck, Petra
AU - Jeschke, Udo
PY - 2019/8
Y1 - 2019/8
N2 - PURPOSE: The objective of this study was to analyze the expression of the glucocorticoid receptor (GR) subtypes GRα and GRβ in placentas affected by intrauterine growth restriction (IUGR).METHODS: We analyzed the sex-specific placental expression of GRα and GRβ in 23 IUGR and 40 control placentas using immunohistochemistry and immunofluorescence. The GR gene, also known as nuclear receptor subfamily 3 group C member 1 (NR3C1), mRNA production in trophoblast-like cell line BeWo after stimulation with prednisolone was analyzed using quantitative polymerase chain reaction (qPCR) and on the protein level using western blot analysis.RESULTS: GR subtypes showed a sex-specific upregulation in placentas from IUGR compared to control placentas. An increased expression of GRα was detectable in female placental tissue, whereas GRβ was increased in males.CONCLUSION: Our data support previous findings suggesting that the glucocorticoid metabolism plays a role in the pathophysiology of IUGR. Furthermore, the data suggest that the underlying molecular mechanisms differ between male and female cases.
AB - PURPOSE: The objective of this study was to analyze the expression of the glucocorticoid receptor (GR) subtypes GRα and GRβ in placentas affected by intrauterine growth restriction (IUGR).METHODS: We analyzed the sex-specific placental expression of GRα and GRβ in 23 IUGR and 40 control placentas using immunohistochemistry and immunofluorescence. The GR gene, also known as nuclear receptor subfamily 3 group C member 1 (NR3C1), mRNA production in trophoblast-like cell line BeWo after stimulation with prednisolone was analyzed using quantitative polymerase chain reaction (qPCR) and on the protein level using western blot analysis.RESULTS: GR subtypes showed a sex-specific upregulation in placentas from IUGR compared to control placentas. An increased expression of GRα was detectable in female placental tissue, whereas GRβ was increased in males.CONCLUSION: Our data support previous findings suggesting that the glucocorticoid metabolism plays a role in the pathophysiology of IUGR. Furthermore, the data suggest that the underlying molecular mechanisms differ between male and female cases.
KW - Journal Article
U2 - 10.1007/s00404-019-05189-7
DO - 10.1007/s00404-019-05189-7
M3 - SCORING: Journal article
C2 - 31089804
VL - 300
SP - 323
EP - 335
JO - ARCH GYNECOL OBSTET
JF - ARCH GYNECOL OBSTET
SN - 0932-0067
IS - 2
ER -