Glioblastoma stem-like cell lines with either maintenance or loss of high-level EGFR amplification, generated via modulation of ligand concentration.
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Glioblastoma stem-like cell lines with either maintenance or loss of high-level EGFR amplification, generated via modulation of ligand concentration. / Schulte, Alexander; Günther, Hauke; Martens, Tobias; Zapf, Svenja; Riethdorf, Sabine; Wülfing, Clemens; Stoupiec, Malgorzata; Westphal, Manfred; Lamszus, Katrin.
in: CLIN CANCER RES, Jahrgang 18, Nr. 7, 7, 2012, S. 1901-1913.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Glioblastoma stem-like cell lines with either maintenance or loss of high-level EGFR amplification, generated via modulation of ligand concentration.
AU - Schulte, Alexander
AU - Günther, Hauke
AU - Martens, Tobias
AU - Zapf, Svenja
AU - Riethdorf, Sabine
AU - Wülfing, Clemens
AU - Stoupiec, Malgorzata
AU - Westphal, Manfred
AU - Lamszus, Katrin
PY - 2012
Y1 - 2012
N2 - Despite the high incidence of epidermal growth factor receptor (EGFR) gene amplification and rearrangement in glioblastomas, no suitable cell line exists that preserves these alterations in vitro and is tumorigenic in immunocompromised mice. On the basis of previous observations that glioblastoma cells cultured with serum lose the EGFR amplification rapidly and that EGF can inhibit the growth of EGFR-amplified tumor cells, we hypothesized that serum-free and EGF-free culture conditions could promote maintenance of the EGFR amplification.
AB - Despite the high incidence of epidermal growth factor receptor (EGFR) gene amplification and rearrangement in glioblastomas, no suitable cell line exists that preserves these alterations in vitro and is tumorigenic in immunocompromised mice. On the basis of previous observations that glioblastoma cells cultured with serum lose the EGFR amplification rapidly and that EGF can inhibit the growth of EGFR-amplified tumor cells, we hypothesized that serum-free and EGF-free culture conditions could promote maintenance of the EGFR amplification.
M3 - SCORING: Journal article
VL - 18
SP - 1901
EP - 1913
JO - CLIN CANCER RES
JF - CLIN CANCER RES
SN - 1078-0432
IS - 7
M1 - 7
ER -