Enhanced expression of the M-type phospholipase A2 receptor in glomeruli correlates with serum receptor antibodies in primary membranous nephropathy.
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Enhanced expression of the M-type phospholipase A2 receptor in glomeruli correlates with serum receptor antibodies in primary membranous nephropathy. / Hoxha, Elion; Kneißler, Ursula; Stege, Gesa; Zahner, Gunther; Thiele, Ina; Panzer, Ulf; Harendza, Sigrid; Helmchen, Udo; Stahl, Rolf A.K.
in: KIDNEY INT, Jahrgang 82, Nr. 7, 7, 2012, S. 797-804.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - Enhanced expression of the M-type phospholipase A2 receptor in glomeruli correlates with serum receptor antibodies in primary membranous nephropathy.
AU - Hoxha, Elion
AU - Kneißler, Ursula
AU - Stege, Gesa
AU - Zahner, Gunther
AU - Thiele, Ina
AU - Panzer, Ulf
AU - Harendza, Sigrid
AU - Helmchen, Udo
AU - Stahl, Rolf A.K.
PY - 2012
Y1 - 2012
N2 - The M-type phospholipase A2 receptor (PLA2R) is the major target antigen in idiopathic membranous nephropathy with detectable autoantibodies in the serum of up to 70% of patients. In retrospective studies, the PLA2R-autoantibody titer in the serum was sometimes negative indicating their measurement alone may be inconclusive. In order to better differentiate between primary and secondary membranous nephropathy, we conducted a prospective study that included 88 patients with a histologic diagnosis of membranous nephropathy. Immunohistochemical analysis for PLA2R was faintly positive in kidneys from normal individuals and patients with various other glomerular injuries. In 61 of the 88 patients, PLA2R expression was strongly positive in glomeruli, and in 60 of these patients PLA2R autoantibodies were also detected in the serum. The 27 patients negative for serum PLA2R autoantibodies were faintly positive for PLA2R staining in glomeruli and in 15 of these patients a secondary cause was found. The remaining 12 patients have a yet undetected secondary cause of membranous nephropathy or have different glomerular antigens other than PLA2R. Thus, increased staining for PLA2R in glomeruli of renal biopsies tightly correlates with the presence of PLA2R autoantibodies in the serum and this may help discriminate between primary and secondary membranous nephropathy.
AB - The M-type phospholipase A2 receptor (PLA2R) is the major target antigen in idiopathic membranous nephropathy with detectable autoantibodies in the serum of up to 70% of patients. In retrospective studies, the PLA2R-autoantibody titer in the serum was sometimes negative indicating their measurement alone may be inconclusive. In order to better differentiate between primary and secondary membranous nephropathy, we conducted a prospective study that included 88 patients with a histologic diagnosis of membranous nephropathy. Immunohistochemical analysis for PLA2R was faintly positive in kidneys from normal individuals and patients with various other glomerular injuries. In 61 of the 88 patients, PLA2R expression was strongly positive in glomeruli, and in 60 of these patients PLA2R autoantibodies were also detected in the serum. The 27 patients negative for serum PLA2R autoantibodies were faintly positive for PLA2R staining in glomeruli and in 15 of these patients a secondary cause was found. The remaining 12 patients have a yet undetected secondary cause of membranous nephropathy or have different glomerular antigens other than PLA2R. Thus, increased staining for PLA2R in glomeruli of renal biopsies tightly correlates with the presence of PLA2R autoantibodies in the serum and this may help discriminate between primary and secondary membranous nephropathy.
KW - Adult
KW - Biopsy
KW - Humans
KW - Male
KW - Aged
KW - Female
KW - Middle Aged
KW - Prospective Studies
KW - Immunohistochemistry
KW - Predictive Value of Tests
KW - Up-Regulation
KW - Reverse Transcriptase Polymerase Chain Reaction
KW - Case-Control Studies
KW - RNA, Messenger/analysis
KW - Creatinine/blood
KW - Biological Markers/blood
KW - Autoantibodies/blood
KW - Glomerulonephritis, Membranous/blood/genetics/immunology/pathology
KW - Kidney Glomerulus/chemistry/immunology/pathology
KW - Proteinuria/blood/immunology
KW - Receptors, Phospholipase A2/analysis/genetics/immunology
KW - Adult
KW - Biopsy
KW - Humans
KW - Male
KW - Aged
KW - Female
KW - Middle Aged
KW - Prospective Studies
KW - Immunohistochemistry
KW - Predictive Value of Tests
KW - Up-Regulation
KW - Reverse Transcriptase Polymerase Chain Reaction
KW - Case-Control Studies
KW - RNA, Messenger/analysis
KW - Creatinine/blood
KW - Biological Markers/blood
KW - Autoantibodies/blood
KW - Glomerulonephritis, Membranous/blood/genetics/immunology/pathology
KW - Kidney Glomerulus/chemistry/immunology/pathology
KW - Proteinuria/blood/immunology
KW - Receptors, Phospholipase A2/analysis/genetics/immunology
M3 - SCORING: Journal article
VL - 82
SP - 797
EP - 804
JO - KIDNEY INT
JF - KIDNEY INT
SN - 0085-2538
IS - 7
M1 - 7
ER -
