Effects of a high-caloric diet and physical exercise on brain metabolite levels a combined proton MRS and histologic study
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Effects of a high-caloric diet and physical exercise on brain metabolite levels a combined proton MRS and histologic study. / Auer, Matthias K; Sack, Markus; Lenz, Jenny N; Jakovcevski, Mira; Biedermann, Sarah V; Falfán-Melgoza, Claudia; Deussing, Jan; Steinle, Jörg; Bielohuby, Maximilian; Bidlingmaier, Martin; Pfister, Frederik; Stalla, Günter K; Ende, Gabriele; Weber-Fahr, Wolfgang; Fuss, Johannes; Gass, Peter.
in: J CEREBR BLOOD F MET, Jahrgang 35, Nr. 4, 04.2015, S. 554-64.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Effects of a high-caloric diet and physical exercise on brain metabolite levels a combined proton MRS and histologic study
AU - Auer, Matthias K
AU - Sack, Markus
AU - Lenz, Jenny N
AU - Jakovcevski, Mira
AU - Biedermann, Sarah V
AU - Falfán-Melgoza, Claudia
AU - Deussing, Jan
AU - Steinle, Jörg
AU - Bielohuby, Maximilian
AU - Bidlingmaier, Martin
AU - Pfister, Frederik
AU - Stalla, Günter K
AU - Ende, Gabriele
AU - Weber-Fahr, Wolfgang
AU - Fuss, Johannes
AU - Gass, Peter
PY - 2015/4
Y1 - 2015/4
N2 - Excessive intake of high-caloric diets as well as subsequent development of obesity and diabetes mellitus may exert a wide range of unfavorable effects on the central nervous system (CNS). It has been suggested that one mechanism in this context is the promotion of neuroinflammation. The potentially harmful effects of such diets were suggested to be mitigated by physical exercise. Here, we conducted a study investigating the effects of physical exercise in a cafeteria-diet mouse model on CNS metabolites by means of in vivo proton magnetic resonance spectroscopy ((1)HMRS). In addition postmortem histologic and real-time (RT)-PCR analyses for inflammatory markers were performed. Cafeteria diet induced obesity and hyperglycemia, which was only partially moderated by exercise. It also induced several changes in CNS metabolites such as reduced hippocampal glutamate (Glu), choline-containing compounds (tCho) and N-acetylaspartate (NAA)+N-acetyl-aspartyl-glutamic acid (NAAG) (tNAA) levels, whereas opposite effects were seen for running. No association of these effects with markers of central inflammation could be observed. These findings suggest that while voluntary wheel running alone is insufficient to prevent the unfavorable peripheral sequelae of the diet, it counteracted many changes in brain metabolites. The observed effects seem to be independent of neuroinflammation.
AB - Excessive intake of high-caloric diets as well as subsequent development of obesity and diabetes mellitus may exert a wide range of unfavorable effects on the central nervous system (CNS). It has been suggested that one mechanism in this context is the promotion of neuroinflammation. The potentially harmful effects of such diets were suggested to be mitigated by physical exercise. Here, we conducted a study investigating the effects of physical exercise in a cafeteria-diet mouse model on CNS metabolites by means of in vivo proton magnetic resonance spectroscopy ((1)HMRS). In addition postmortem histologic and real-time (RT)-PCR analyses for inflammatory markers were performed. Cafeteria diet induced obesity and hyperglycemia, which was only partially moderated by exercise. It also induced several changes in CNS metabolites such as reduced hippocampal glutamate (Glu), choline-containing compounds (tCho) and N-acetylaspartate (NAA)+N-acetyl-aspartyl-glutamic acid (NAAG) (tNAA) levels, whereas opposite effects were seen for running. No association of these effects with markers of central inflammation could be observed. These findings suggest that while voluntary wheel running alone is insufficient to prevent the unfavorable peripheral sequelae of the diet, it counteracted many changes in brain metabolites. The observed effects seem to be independent of neuroinflammation.
KW - Animals
KW - Aspartic Acid
KW - Brain
KW - Carbohydrate Metabolism
KW - Diet
KW - Dipeptides
KW - Energy Intake
KW - Fatty Acids
KW - Glucose
KW - Glutamic Acid
KW - Hyperglycemia
KW - Insulin
KW - Magnetic Resonance Spectroscopy
KW - Male
KW - Mice
KW - Mice, Inbred C57BL
KW - Obesity
KW - Physical Conditioning, Animal
KW - Protons
KW - Running
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1038/jcbfm.2014.231
DO - 10.1038/jcbfm.2014.231
M3 - SCORING: Journal article
C2 - 25564238
VL - 35
SP - 554
EP - 564
JO - J CEREBR BLOOD F MET
JF - J CEREBR BLOOD F MET
SN - 0271-678X
IS - 4
ER -