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DNA methylation profiling allows for characterization of atrial and ventricular cardiac tissues and hiPSC-CMs

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DNA methylation profiling allows for characterization of atrial and ventricular cardiac tissues and hiPSC-CMs. / Hoff, Kirstin; Lemme, Marta; Kahlert, Anne-Karin; Runde, Kerstin; Audain, Enrique; Schuster, Dorit; Scheewe, Jens; Attmann, Tim; Pickardt, Thomas; Caliebe, Almuth; Siebert, Reiner; Kramer, Hans-Heiner; Milting, Hendrik; Hansen, Arne; Ammerpohl, Ole; Hitz, Marc-Phillip.

in: CLIN EPIGENETICS, Jahrgang 11, Nr. 1, 11.06.2019, S. 89.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Hoff, K, Lemme, M, Kahlert, A-K, Runde, K, Audain, E, Schuster, D, Scheewe, J, Attmann, T, Pickardt, T, Caliebe, A, Siebert, R, Kramer, H-H, Milting, H, Hansen, A, Ammerpohl, O & Hitz, M-P 2019, 'DNA methylation profiling allows for characterization of atrial and ventricular cardiac tissues and hiPSC-CMs', CLIN EPIGENETICS, Jg. 11, Nr. 1, S. 89. https://doi.org/10.1186/s13148-019-0679-0

APA

Hoff, K., Lemme, M., Kahlert, A-K., Runde, K., Audain, E., Schuster, D., Scheewe, J., Attmann, T., Pickardt, T., Caliebe, A., Siebert, R., Kramer, H-H., Milting, H., Hansen, A., Ammerpohl, O., & Hitz, M-P. (2019). DNA methylation profiling allows for characterization of atrial and ventricular cardiac tissues and hiPSC-CMs. CLIN EPIGENETICS, 11(1), 89. https://doi.org/10.1186/s13148-019-0679-0

Vancouver

Hoff K, Lemme M, Kahlert A-K, Runde K, Audain E, Schuster D et al. DNA methylation profiling allows for characterization of atrial and ventricular cardiac tissues and hiPSC-CMs. CLIN EPIGENETICS. 2019 Jun 11;11(1):89. https://doi.org/10.1186/s13148-019-0679-0

Bibtex

@article{20267bb2b13447c69d0ddf5b7320c22c,
title = "DNA methylation profiling allows for characterization of atrial and ventricular cardiac tissues and hiPSC-CMs",
abstract = "BACKGROUND: Cardiac disease modelling using human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) requires thorough insight into cardiac cell type differentiation processes. However, current methods to discriminate different cardiac cell types are mostly time-consuming, are costly and often provide imprecise phenotypic evaluation. DNA methylation plays a critical role during early heart development and cardiac cellular specification. We therefore investigated the DNA methylation pattern in different cardiac tissues to identify CpG loci for further cardiac cell type characterization.RESULTS: An array-based genome-wide DNA methylation analysis using Illumina Infinium HumanMethylation450 BeadChips led to the identification of 168 differentially methylated CpG loci in atrial and ventricular human heart tissue samples (n = 49) from different patients with congenital heart defects (CHD). Systematic evaluation of atrial-ventricular DNA methylation pattern in cardiac tissues in an independent sample cohort of non-failing donor hearts and cardiac patients using bisulfite pyrosequencing helped us to define a subset of 16 differentially methylated CpG loci enabling precise characterization of human atrial and ventricular cardiac tissue samples. This defined set of reproducible cardiac tissue-specific DNA methylation sites allowed us to consistently detect the cellular identity of hiPSC-CM subtypes.CONCLUSION: Testing DNA methylation of only a small set of defined CpG sites thus makes it possible to distinguish atrial and ventricular cardiac tissues and cardiac atrial and ventricular subtypes of hiPSC-CMs. This method represents a rapid and reliable system for phenotypic characterization of in vitro-generated cardiomyocytes and opens new opportunities for cardiovascular research and patient-specific therapy.",
author = "Kirstin Hoff and Marta Lemme and Anne-Karin Kahlert and Kerstin Runde and Enrique Audain and Dorit Schuster and Jens Scheewe and Tim Attmann and Thomas Pickardt and Almuth Caliebe and Reiner Siebert and Hans-Heiner Kramer and Hendrik Milting and Arne Hansen and Ole Ammerpohl and Marc-Phillip Hitz",
year = "2019",
month = jun,
day = "11",
doi = "10.1186/s13148-019-0679-0",
language = "English",
volume = "11",
pages = "89",
journal = "CLIN EPIGENETICS",
issn = "1868-7075",
publisher = "Springer",
number = "1",

}

RIS

TY - JOUR

T1 - DNA methylation profiling allows for characterization of atrial and ventricular cardiac tissues and hiPSC-CMs

AU - Hoff, Kirstin

AU - Lemme, Marta

AU - Kahlert, Anne-Karin

AU - Runde, Kerstin

AU - Audain, Enrique

AU - Schuster, Dorit

AU - Scheewe, Jens

AU - Attmann, Tim

AU - Pickardt, Thomas

AU - Caliebe, Almuth

AU - Siebert, Reiner

AU - Kramer, Hans-Heiner

AU - Milting, Hendrik

AU - Hansen, Arne

AU - Ammerpohl, Ole

AU - Hitz, Marc-Phillip

PY - 2019/6/11

Y1 - 2019/6/11

N2 - BACKGROUND: Cardiac disease modelling using human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) requires thorough insight into cardiac cell type differentiation processes. However, current methods to discriminate different cardiac cell types are mostly time-consuming, are costly and often provide imprecise phenotypic evaluation. DNA methylation plays a critical role during early heart development and cardiac cellular specification. We therefore investigated the DNA methylation pattern in different cardiac tissues to identify CpG loci for further cardiac cell type characterization.RESULTS: An array-based genome-wide DNA methylation analysis using Illumina Infinium HumanMethylation450 BeadChips led to the identification of 168 differentially methylated CpG loci in atrial and ventricular human heart tissue samples (n = 49) from different patients with congenital heart defects (CHD). Systematic evaluation of atrial-ventricular DNA methylation pattern in cardiac tissues in an independent sample cohort of non-failing donor hearts and cardiac patients using bisulfite pyrosequencing helped us to define a subset of 16 differentially methylated CpG loci enabling precise characterization of human atrial and ventricular cardiac tissue samples. This defined set of reproducible cardiac tissue-specific DNA methylation sites allowed us to consistently detect the cellular identity of hiPSC-CM subtypes.CONCLUSION: Testing DNA methylation of only a small set of defined CpG sites thus makes it possible to distinguish atrial and ventricular cardiac tissues and cardiac atrial and ventricular subtypes of hiPSC-CMs. This method represents a rapid and reliable system for phenotypic characterization of in vitro-generated cardiomyocytes and opens new opportunities for cardiovascular research and patient-specific therapy.

AB - BACKGROUND: Cardiac disease modelling using human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) requires thorough insight into cardiac cell type differentiation processes. However, current methods to discriminate different cardiac cell types are mostly time-consuming, are costly and often provide imprecise phenotypic evaluation. DNA methylation plays a critical role during early heart development and cardiac cellular specification. We therefore investigated the DNA methylation pattern in different cardiac tissues to identify CpG loci for further cardiac cell type characterization.RESULTS: An array-based genome-wide DNA methylation analysis using Illumina Infinium HumanMethylation450 BeadChips led to the identification of 168 differentially methylated CpG loci in atrial and ventricular human heart tissue samples (n = 49) from different patients with congenital heart defects (CHD). Systematic evaluation of atrial-ventricular DNA methylation pattern in cardiac tissues in an independent sample cohort of non-failing donor hearts and cardiac patients using bisulfite pyrosequencing helped us to define a subset of 16 differentially methylated CpG loci enabling precise characterization of human atrial and ventricular cardiac tissue samples. This defined set of reproducible cardiac tissue-specific DNA methylation sites allowed us to consistently detect the cellular identity of hiPSC-CM subtypes.CONCLUSION: Testing DNA methylation of only a small set of defined CpG sites thus makes it possible to distinguish atrial and ventricular cardiac tissues and cardiac atrial and ventricular subtypes of hiPSC-CMs. This method represents a rapid and reliable system for phenotypic characterization of in vitro-generated cardiomyocytes and opens new opportunities for cardiovascular research and patient-specific therapy.

U2 - 10.1186/s13148-019-0679-0

DO - 10.1186/s13148-019-0679-0

M3 - SCORING: Journal article

C2 - 31186048

VL - 11

SP - 89

JO - CLIN EPIGENETICS

JF - CLIN EPIGENETICS

SN - 1868-7075

IS - 1

ER -