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Differentially expressed genes in neurofibromatosis 1-associated neurofibromas and malignant peripheral nerve sheath tumors

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Differentially expressed genes in neurofibromatosis 1-associated neurofibromas and malignant peripheral nerve sheath tumors. / Holtkamp, Nikola; Mautner, Victor-F; Friedrich, Reinhard E; Harder, Anja; Hartmann, Christian; Theallier-Janko, Agota; Hoffmann, Karl T; von Deimling, Andreas.

in: ACTA NEUROPATHOL, Jahrgang 107, Nr. 2, 01.02.2004, S. 159-68.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Holtkamp, N, Mautner, V-F, Friedrich, RE, Harder, A, Hartmann, C, Theallier-Janko, A, Hoffmann, KT & von Deimling, A 2004, 'Differentially expressed genes in neurofibromatosis 1-associated neurofibromas and malignant peripheral nerve sheath tumors', ACTA NEUROPATHOL, Jg. 107, Nr. 2, S. 159-68. https://doi.org/10.1007/s00401-003-0797-8

APA

Holtkamp, N., Mautner, V-F., Friedrich, R. E., Harder, A., Hartmann, C., Theallier-Janko, A., Hoffmann, K. T., & von Deimling, A. (2004). Differentially expressed genes in neurofibromatosis 1-associated neurofibromas and malignant peripheral nerve sheath tumors. ACTA NEUROPATHOL, 107(2), 159-68. https://doi.org/10.1007/s00401-003-0797-8

Vancouver

Holtkamp N, Mautner V-F, Friedrich RE, Harder A, Hartmann C, Theallier-Janko A et al. Differentially expressed genes in neurofibromatosis 1-associated neurofibromas and malignant peripheral nerve sheath tumors. ACTA NEUROPATHOL. 2004 Feb 1;107(2):159-68. https://doi.org/10.1007/s00401-003-0797-8

Bibtex

@article{7e589100fca24b988cfeeea199777786,
title = "Differentially expressed genes in neurofibromatosis 1-associated neurofibromas and malignant peripheral nerve sheath tumors",
abstract = "Neurofibromas represent one of the hallmarks of neurofibromatosis 1 (NF1) patients. Tumor progression of neurofibromas to malignant peripheral nerve sheath tumors (MPNST) is a frequent and life threatening complication. To learn more about processes involved in malignant transformation, we evaluated differential gene expression in plexiform neurofibroma and MPNST from the same NF1 patient. Suppression subtractive hybridization (SSH) yielded 133 differentially expressed genes confirmed by reverse Northern blotting. Virtual Northern blots were employed to validate 23 genes. To independently verify differential expression, immunohistochemical analyses with antibodies to matrix metalloproteinase 13 (MMP13), platelet-derived growth factor receptor alpha (PDGFRA) and fibronectin (FN1) were performed on 9 dermal and 9 plexiform neurofibromas and 16 MPNST from 19 NF1 patients. All three proteins proved to be up-regulated in MPNST. MMP13 expression was observed in 44% of MPNST but was absent in neurofibromas. PDGFRA was expressed in all tumors, but the number of cells expressing it was below 30% in neurofibromas and over 50% in MPNST. Likewise, FN1 was expressed in all tumors, but less than 30% of the cells in neurofibromas and more than 70% of the cells in MPNST exhibited antibody binding. Our data point to several genes not previously recognized to be differentially expressed, and provide a framework for future studies on progression-associated gene expression in low- and high-grade nerve sheath tumors.",
keywords = "Adult, Blotting, Northern, Collagenases, Female, Fibronectins, Gene Expression, Humans, Hybridization, Genetic, Immunohistochemistry, Intercellular Signaling Peptides and Proteins, Magnetic Resonance Imaging, Matrix Metalloproteinase 13, Nerve Sheath Neoplasms, Neurofibroma, Neurofibromatosis 1, Pelvis, Phosphoproteins, Platelet-Derived Growth Factor, Sequence Analysis",
author = "Nikola Holtkamp and Victor-F Mautner and Friedrich, {Reinhard E} and Anja Harder and Christian Hartmann and Agota Theallier-Janko and Hoffmann, {Karl T} and {von Deimling}, Andreas",
year = "2004",
month = feb,
day = "1",
doi = "10.1007/s00401-003-0797-8",
language = "English",
volume = "107",
pages = "159--68",
journal = "ACTA NEUROPATHOL",
issn = "0001-6322",
publisher = "Springer",
number = "2",

}

RIS

TY - JOUR

T1 - Differentially expressed genes in neurofibromatosis 1-associated neurofibromas and malignant peripheral nerve sheath tumors

AU - Holtkamp, Nikola

AU - Mautner, Victor-F

AU - Friedrich, Reinhard E

AU - Harder, Anja

AU - Hartmann, Christian

AU - Theallier-Janko, Agota

AU - Hoffmann, Karl T

AU - von Deimling, Andreas

PY - 2004/2/1

Y1 - 2004/2/1

N2 - Neurofibromas represent one of the hallmarks of neurofibromatosis 1 (NF1) patients. Tumor progression of neurofibromas to malignant peripheral nerve sheath tumors (MPNST) is a frequent and life threatening complication. To learn more about processes involved in malignant transformation, we evaluated differential gene expression in plexiform neurofibroma and MPNST from the same NF1 patient. Suppression subtractive hybridization (SSH) yielded 133 differentially expressed genes confirmed by reverse Northern blotting. Virtual Northern blots were employed to validate 23 genes. To independently verify differential expression, immunohistochemical analyses with antibodies to matrix metalloproteinase 13 (MMP13), platelet-derived growth factor receptor alpha (PDGFRA) and fibronectin (FN1) were performed on 9 dermal and 9 plexiform neurofibromas and 16 MPNST from 19 NF1 patients. All three proteins proved to be up-regulated in MPNST. MMP13 expression was observed in 44% of MPNST but was absent in neurofibromas. PDGFRA was expressed in all tumors, but the number of cells expressing it was below 30% in neurofibromas and over 50% in MPNST. Likewise, FN1 was expressed in all tumors, but less than 30% of the cells in neurofibromas and more than 70% of the cells in MPNST exhibited antibody binding. Our data point to several genes not previously recognized to be differentially expressed, and provide a framework for future studies on progression-associated gene expression in low- and high-grade nerve sheath tumors.

AB - Neurofibromas represent one of the hallmarks of neurofibromatosis 1 (NF1) patients. Tumor progression of neurofibromas to malignant peripheral nerve sheath tumors (MPNST) is a frequent and life threatening complication. To learn more about processes involved in malignant transformation, we evaluated differential gene expression in plexiform neurofibroma and MPNST from the same NF1 patient. Suppression subtractive hybridization (SSH) yielded 133 differentially expressed genes confirmed by reverse Northern blotting. Virtual Northern blots were employed to validate 23 genes. To independently verify differential expression, immunohistochemical analyses with antibodies to matrix metalloproteinase 13 (MMP13), platelet-derived growth factor receptor alpha (PDGFRA) and fibronectin (FN1) were performed on 9 dermal and 9 plexiform neurofibromas and 16 MPNST from 19 NF1 patients. All three proteins proved to be up-regulated in MPNST. MMP13 expression was observed in 44% of MPNST but was absent in neurofibromas. PDGFRA was expressed in all tumors, but the number of cells expressing it was below 30% in neurofibromas and over 50% in MPNST. Likewise, FN1 was expressed in all tumors, but less than 30% of the cells in neurofibromas and more than 70% of the cells in MPNST exhibited antibody binding. Our data point to several genes not previously recognized to be differentially expressed, and provide a framework for future studies on progression-associated gene expression in low- and high-grade nerve sheath tumors.

KW - Adult

KW - Blotting, Northern

KW - Collagenases

KW - Female

KW - Fibronectins

KW - Gene Expression

KW - Humans

KW - Hybridization, Genetic

KW - Immunohistochemistry

KW - Intercellular Signaling Peptides and Proteins

KW - Magnetic Resonance Imaging

KW - Matrix Metalloproteinase 13

KW - Nerve Sheath Neoplasms

KW - Neurofibroma

KW - Neurofibromatosis 1

KW - Pelvis

KW - Phosphoproteins

KW - Platelet-Derived Growth Factor

KW - Sequence Analysis

U2 - 10.1007/s00401-003-0797-8

DO - 10.1007/s00401-003-0797-8

M3 - SCORING: Journal article

C2 - 14673600

VL - 107

SP - 159

EP - 168

JO - ACTA NEUROPATHOL

JF - ACTA NEUROPATHOL

SN - 0001-6322

IS - 2

ER -