Diadenosine phosphates and the physiological control of blood pressure
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Diadenosine phosphates and the physiological control of blood pressure. / Schlüter, H; Offers, E; Brüggemann, G; van der Giet, M; Tepel, M; Nordhoff, E; Karas, M; Spieker, C; Witzel, H; Zidek, W.
in: NATURE, Jahrgang 367, Nr. 6459, 13.01.1994, S. 186-8.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - Diadenosine phosphates and the physiological control of blood pressure
AU - Schlüter, H
AU - Offers, E
AU - Brüggemann, G
AU - van der Giet, M
AU - Tepel, M
AU - Nordhoff, E
AU - Karas, M
AU - Spieker, C
AU - Witzel, H
AU - Zidek, W
PY - 1994/1/13
Y1 - 1994/1/13
N2 - Our understanding of the regulation of vascular tone has been extended since the identification of vasoactive agents such as the atrial natriuretic peptides, endothelial-derived relaxing factor and endothelin. Unidentified vasopressive agents have been found in platelets. Here we isolate these vasopressors and identify them as diadenosine pentaphosphate (AP5A) and diadenosine hexaphosphate (AP6A) by chromatography, mass spectrometry, ultraviolet spectroscopy and enzymatic cleavage. In the vasculature of isolated perfused rat kidney, both diadenosine phosphates were active at a concentration of 10(-9) M; in aortic rings, contractions were elicited at 10(-8) M. Intra-aortic injection in the rat caused a prolonged increase in blood pressure. We conclude that AP5A and AP6A may play a part in local vasoregulation and possibly in the regulation of blood pressure.
AB - Our understanding of the regulation of vascular tone has been extended since the identification of vasoactive agents such as the atrial natriuretic peptides, endothelial-derived relaxing factor and endothelin. Unidentified vasopressive agents have been found in platelets. Here we isolate these vasopressors and identify them as diadenosine pentaphosphate (AP5A) and diadenosine hexaphosphate (AP6A) by chromatography, mass spectrometry, ultraviolet spectroscopy and enzymatic cleavage. In the vasculature of isolated perfused rat kidney, both diadenosine phosphates were active at a concentration of 10(-9) M; in aortic rings, contractions were elicited at 10(-8) M. Intra-aortic injection in the rat caused a prolonged increase in blood pressure. We conclude that AP5A and AP6A may play a part in local vasoregulation and possibly in the regulation of blood pressure.
KW - 5'-Nucleotidase
KW - Alkaline Phosphatase
KW - Animals
KW - Aorta
KW - Blood Platelets
KW - Blood Pressure
KW - Chromatography, Liquid
KW - Dinucleoside Phosphates
KW - Exonucleases
KW - In Vitro Techniques
KW - Kidney
KW - Mass Spectrometry
KW - Nucleotidases
KW - Phosphodiesterase I
KW - Phosphoric Diester Hydrolases
KW - Rats
KW - Spectrophotometry, Ultraviolet
KW - Vasoconstriction
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1038/367186a0
DO - 10.1038/367186a0
M3 - SCORING: Journal article
C2 - 8114917
VL - 367
SP - 186
EP - 188
JO - NATURE
JF - NATURE
SN - 0028-0836
IS - 6459
ER -