Dermatan sulfotransferase Chst14/D4st1, but not chondroitin sulfotransferase Chst11/C4st1, regulates proliferation and neurogenesis of neural progenitor cells.
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Dermatan sulfotransferase Chst14/D4st1, but not chondroitin sulfotransferase Chst11/C4st1, regulates proliferation and neurogenesis of neural progenitor cells. / Bian, Shan; Akyüz, Nuray; Bernreuther, Christian; Loers, Gabriele; Laczynska, Ewa; Jakovcevski, Igor; Schachner, Melitta.
in: J CELL SCI, Jahrgang 124, Nr. Pt 23, Pt 23, 2011, S. 4051-4063.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Dermatan sulfotransferase Chst14/D4st1, but not chondroitin sulfotransferase Chst11/C4st1, regulates proliferation and neurogenesis of neural progenitor cells.
AU - Bian, Shan
AU - Akyüz, Nuray
AU - Bernreuther, Christian
AU - Loers, Gabriele
AU - Laczynska, Ewa
AU - Jakovcevski, Igor
AU - Schachner, Melitta
PY - 2011
Y1 - 2011
N2 - Chondroitin sulfates (CSs) and dermatan sulfates (DSs) are enriched in the microenvironment of neural stem cells (NSCs) during development and in the adult neurogenic niche, and have been implicated in mechanisms governing neural precursor migration, proliferation and differentiation. In contrast to previous studies, in which a chondroitinaseABC-dependent unselective deglycosylation of both CSs and DSs was performed, we used chondroitin 4-O-sulfotransferase-1 (Chst11/C4st1)- and dermatan 4-O-sulfotransferase-1 (Chst14/D4st1)-deficient NSCs specific for CSs and DSs, respectively, to investigate the involvement of specific sulfation profiles of CS and DS chains, and thus the potentially distinct roles of CSs and DSs in NSC biology. In comparison to wild-type controls, deficiency for Chst14 resulted in decreased neurogenesis and diminished proliferation of NSCs accompanied by increased expression of GLAST and decreased expression of Mash-1, and an upregulation of the expression of the receptors for fibroblast growth factor-2 (FGF-2) and epidermal growth factor (EGF). By contrast, deficiency in Chst11 did not influence NSC proliferation, migration or differentiation. These observations indicate for the first time that CSs and DSs play distinct roles in the self-renewal and differentiation of NSCs.
AB - Chondroitin sulfates (CSs) and dermatan sulfates (DSs) are enriched in the microenvironment of neural stem cells (NSCs) during development and in the adult neurogenic niche, and have been implicated in mechanisms governing neural precursor migration, proliferation and differentiation. In contrast to previous studies, in which a chondroitinaseABC-dependent unselective deglycosylation of both CSs and DSs was performed, we used chondroitin 4-O-sulfotransferase-1 (Chst11/C4st1)- and dermatan 4-O-sulfotransferase-1 (Chst14/D4st1)-deficient NSCs specific for CSs and DSs, respectively, to investigate the involvement of specific sulfation profiles of CS and DS chains, and thus the potentially distinct roles of CSs and DSs in NSC biology. In comparison to wild-type controls, deficiency for Chst14 resulted in decreased neurogenesis and diminished proliferation of NSCs accompanied by increased expression of GLAST and decreased expression of Mash-1, and an upregulation of the expression of the receptors for fibroblast growth factor-2 (FGF-2) and epidermal growth factor (EGF). By contrast, deficiency in Chst11 did not influence NSC proliferation, migration or differentiation. These observations indicate for the first time that CSs and DSs play distinct roles in the self-renewal and differentiation of NSCs.
KW - Animals
KW - Immunohistochemistry
KW - Mice
KW - Mice, Inbred C57BL
KW - Cell Movement
KW - Apoptosis
KW - Cell Differentiation
KW - Cell Proliferation
KW - Basic Helix-Loop-Helix Transcription Factors/genetics/metabolism
KW - Bromodeoxyuridine/administration & dosage/pharmacology
KW - Embryo, Mammalian/cytology/drug effects/metabolism
KW - Excitatory Amino Acid Transporter 1/genetics/metabolism
KW - Gene Expression Regulation, Enzymologic
KW - Neural Stem Cells/cytology/drug effects/enzymology
KW - Neurogenesis
KW - Receptor, Epidermal Growth Factor/genetics/metabolism
KW - Receptor, Fibroblast Growth Factor, Type 2/genetics/metabolism
KW - Stem Cell Niche
KW - Sulfotransferases/genetics/metabolism
KW - Animals
KW - Immunohistochemistry
KW - Mice
KW - Mice, Inbred C57BL
KW - Cell Movement
KW - Apoptosis
KW - Cell Differentiation
KW - Cell Proliferation
KW - Basic Helix-Loop-Helix Transcription Factors/genetics/metabolism
KW - Bromodeoxyuridine/administration & dosage/pharmacology
KW - Embryo, Mammalian/cytology/drug effects/metabolism
KW - Excitatory Amino Acid Transporter 1/genetics/metabolism
KW - Gene Expression Regulation, Enzymologic
KW - Neural Stem Cells/cytology/drug effects/enzymology
KW - Neurogenesis
KW - Receptor, Epidermal Growth Factor/genetics/metabolism
KW - Receptor, Fibroblast Growth Factor, Type 2/genetics/metabolism
KW - Stem Cell Niche
KW - Sulfotransferases/genetics/metabolism
M3 - SCORING: Journal article
VL - 124
SP - 4051
EP - 4063
JO - J CELL SCI
JF - J CELL SCI
SN - 0021-9533
IS - Pt 23
M1 - Pt 23
ER -
