Depletion of CD4+ T cells during immunization with nonviable Listeria monocytogenes causes enhanced CD8+ T cell-mediated protection against listeriosis
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Depletion of CD4+ T cells during immunization with nonviable Listeria monocytogenes causes enhanced CD8+ T cell-mediated protection against listeriosis. / Kursar, Mischo; Köhler, Anne; Kaufmann, Stefan H E; Mittrücker, Hans-Willi.
in: J IMMUNOL, Jahrgang 172, Nr. 5, 01.03.2004, S. 3167-72.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Depletion of CD4+ T cells during immunization with nonviable Listeria monocytogenes causes enhanced CD8+ T cell-mediated protection against listeriosis
AU - Kursar, Mischo
AU - Köhler, Anne
AU - Kaufmann, Stefan H E
AU - Mittrücker, Hans-Willi
PY - 2004/3/1
Y1 - 2004/3/1
N2 - Immunization of mice with nonviable Listeria monocytogenes generates an insufficient CD8(+) T cell response and consequently only limited protection against subsequent L. monocytogenes infection. We have recently demonstrated that depletion of regulatory CD4(+) T cells during immunization significantly enhances CD8(+) T cell responses. In the present study, we determined the impact of CD4(+) T cell depletion on the CD8(+) T cell response against heat-killed Listeria. Treatment of mice with anti-CD4 mAb during boost immunization with heat-killed Listeria significantly increased numbers of Listeria-specific CD8(+) T cells and improved protection against subsequent infection with L. monocytogenes. During challenge infection, numbers of Listeria-specific CD8(+) T cells were enhanced, and these cells expressed effector functions in terms of IFN-gamma production. In summary, we demonstrate that combining nonviable L. monocytogenes vaccination and CD4(+) T cell depletion improves generation of long-lasting and functional Listeria-specific CD8(+) memory T cells.
AB - Immunization of mice with nonviable Listeria monocytogenes generates an insufficient CD8(+) T cell response and consequently only limited protection against subsequent L. monocytogenes infection. We have recently demonstrated that depletion of regulatory CD4(+) T cells during immunization significantly enhances CD8(+) T cell responses. In the present study, we determined the impact of CD4(+) T cell depletion on the CD8(+) T cell response against heat-killed Listeria. Treatment of mice with anti-CD4 mAb during boost immunization with heat-killed Listeria significantly increased numbers of Listeria-specific CD8(+) T cells and improved protection against subsequent infection with L. monocytogenes. During challenge infection, numbers of Listeria-specific CD8(+) T cells were enhanced, and these cells expressed effector functions in terms of IFN-gamma production. In summary, we demonstrate that combining nonviable L. monocytogenes vaccination and CD4(+) T cell depletion improves generation of long-lasting and functional Listeria-specific CD8(+) memory T cells.
KW - Animals
KW - Antibodies, Monoclonal
KW - Antigens, CD4
KW - Bacterial Vaccines
KW - CD4-Positive T-Lymphocytes
KW - CD8-Positive T-Lymphocytes
KW - Cytotoxicity, Immunologic
KW - Immunization, Secondary
KW - Injections, Intraperitoneal
KW - Listeria monocytogenes
KW - Listeriosis
KW - Lymphocyte Activation
KW - Lymphocyte Count
KW - Lymphocyte Depletion
KW - Mice
KW - Mice, Inbred BALB C
M3 - SCORING: Journal article
C2 - 14978123
VL - 172
SP - 3167
EP - 3172
JO - J IMMUNOL
JF - J IMMUNOL
SN - 0022-1767
IS - 5
ER -