Critical role for miR-181a/b-1 in agonist selection of invariant natural killer T cells
Standard
Critical role for miR-181a/b-1 in agonist selection of invariant natural killer T cells. / Ziętara, Natalia; Łyszkiewicz, Marcin; Witzlau, Katrin; Naumann, Ronald; Hurwitz, Robert; Langemeier, Jörg; Bohne, Jens; Sandrock, Inga; Ballmaier, Matthias; Weiss, Siegfried; Prinz, Immo; Krueger, Andreas.
in: P NATL ACAD SCI USA, Jahrgang 110, Nr. 18, 30.04.2013, S. 7407-12.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Critical role for miR-181a/b-1 in agonist selection of invariant natural killer T cells
AU - Ziętara, Natalia
AU - Łyszkiewicz, Marcin
AU - Witzlau, Katrin
AU - Naumann, Ronald
AU - Hurwitz, Robert
AU - Langemeier, Jörg
AU - Bohne, Jens
AU - Sandrock, Inga
AU - Ballmaier, Matthias
AU - Weiss, Siegfried
AU - Prinz, Immo
AU - Krueger, Andreas
PY - 2013/4/30
Y1 - 2013/4/30
N2 - T-cell receptor (TCR) signal strength determines selection and lineage fate at the CD4(+)CD8(+) double-positive stage of intrathymic T-cell development. Members of the miR-181 family constitute the most abundantly expressed microRNA at this stage of T-cell development. Here we show that deletion of miR-181a/b-1 reduced the responsiveness of double-positive thymocytes to TCR signals and virtually abrogated early invariant natural killer T (iNKT) cell development, resulting in a dramatic reduction in iNKT cell numbers in thymus as well as in the periphery. Increased concentrations of agonist ligand rescued iNKT cell development in miR-181a/b-1(-/-) mice. Our results define a critical role of miR-181a/b-1 in early iNKT cell development and show that miR-181a/b-1 sets a TCR signaling threshold for agonist selection.
AB - T-cell receptor (TCR) signal strength determines selection and lineage fate at the CD4(+)CD8(+) double-positive stage of intrathymic T-cell development. Members of the miR-181 family constitute the most abundantly expressed microRNA at this stage of T-cell development. Here we show that deletion of miR-181a/b-1 reduced the responsiveness of double-positive thymocytes to TCR signals and virtually abrogated early invariant natural killer T (iNKT) cell development, resulting in a dramatic reduction in iNKT cell numbers in thymus as well as in the periphery. Increased concentrations of agonist ligand rescued iNKT cell development in miR-181a/b-1(-/-) mice. Our results define a critical role of miR-181a/b-1 in early iNKT cell development and show that miR-181a/b-1 sets a TCR signaling threshold for agonist selection.
KW - Animals
KW - Cell Proliferation
KW - Clonal Selection, Antigen-Mediated/immunology
KW - Ligands
KW - Mice
KW - Mice, Inbred C57BL
KW - MicroRNAs/genetics
KW - Natural Killer T-Cells/cytology
KW - Receptors, Antigen, T-Cell, alpha-beta/metabolism
U2 - 10.1073/pnas.1221984110
DO - 10.1073/pnas.1221984110
M3 - SCORING: Journal article
C2 - 23589855
VL - 110
SP - 7407
EP - 7412
JO - P NATL ACAD SCI USA
JF - P NATL ACAD SCI USA
SN - 0027-8424
IS - 18
ER -