Comparison of Cytomegalovirus-Specific Immune Cell Response to Proteins versus Peptides Using an IFN-γ ELISpot Assay after Hematopoietic Stem Cell Transplantation
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Comparison of Cytomegalovirus-Specific Immune Cell Response to Proteins versus Peptides Using an IFN-γ ELISpot Assay after Hematopoietic Stem Cell Transplantation. / Wagner-Drouet, Eva; Teschner, Daniel; Wolschke, Christine; Schäfer-Eckart, Kerstin; Gärtner, Johannes; Mielke, Stephan; Schreder, Martin; Kobbe, Guido; Hilgendorf, Inken; Klein, Stefan; Verbeek, Mareike; Ditschkowski, Markus; Koch, Martina; Lindemann, Monika; Schmidt, Traudel; Rascle, Anne; Barabas, Sascha; Deml, Ludwig; Wagner, Ralf; Wolff, Daniel.
in: DIAGNOSTICS, Jahrgang 11, Nr. 2, 312, 15.02.2021.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - Comparison of Cytomegalovirus-Specific Immune Cell Response to Proteins versus Peptides Using an IFN-γ ELISpot Assay after Hematopoietic Stem Cell Transplantation
AU - Wagner-Drouet, Eva
AU - Teschner, Daniel
AU - Wolschke, Christine
AU - Schäfer-Eckart, Kerstin
AU - Gärtner, Johannes
AU - Mielke, Stephan
AU - Schreder, Martin
AU - Kobbe, Guido
AU - Hilgendorf, Inken
AU - Klein, Stefan
AU - Verbeek, Mareike
AU - Ditschkowski, Markus
AU - Koch, Martina
AU - Lindemann, Monika
AU - Schmidt, Traudel
AU - Rascle, Anne
AU - Barabas, Sascha
AU - Deml, Ludwig
AU - Wagner, Ralf
AU - Wolff, Daniel
PY - 2021/2/15
Y1 - 2021/2/15
N2 - Cytomegalovirus (CMV) infection is a major cause of morbidity and mortality following hematopoietic stem cell transplantation (HSCT). Measuring CMV-specific cellular immunity may improve the risk stratification and management of patients. IFN-γ ELISpot assays, based on the stimulation of peripheral blood mononuclear cells with CMV pp65 and IE-1 proteins or peptides, have been validated in clinical settings. However, it remains unclear to which extend the T-cell response to synthetic peptides reflect that mediated by full-length proteins processed by antigen-presenting cells. We compared the stimulating ability of pp65 and IE-1 proteins and corresponding overlapping peptides in 16 HSCT recipients using a standardized IFN-γ ELISpot assay. Paired qualitative test results showed an overall 74.4% concordance. Discordant results were mainly due to low-response tests, with one exception. One patient with early CMV reactivation and graft-versus-host disease, sustained CMV DNAemia and high CD8+ counts showed successive negative protein-based ELISpot results but a high and sustained response to IE-1 peptides. Our results suggest that the response to exogenous proteins, which involves their uptake and processing by antigen-presenting cells, more closely reflects the physiological response to CMV infection, while the response to exogenous peptides may lead to artificial in vitro T-cell responses, especially in strongly immunosuppressed patients.
AB - Cytomegalovirus (CMV) infection is a major cause of morbidity and mortality following hematopoietic stem cell transplantation (HSCT). Measuring CMV-specific cellular immunity may improve the risk stratification and management of patients. IFN-γ ELISpot assays, based on the stimulation of peripheral blood mononuclear cells with CMV pp65 and IE-1 proteins or peptides, have been validated in clinical settings. However, it remains unclear to which extend the T-cell response to synthetic peptides reflect that mediated by full-length proteins processed by antigen-presenting cells. We compared the stimulating ability of pp65 and IE-1 proteins and corresponding overlapping peptides in 16 HSCT recipients using a standardized IFN-γ ELISpot assay. Paired qualitative test results showed an overall 74.4% concordance. Discordant results were mainly due to low-response tests, with one exception. One patient with early CMV reactivation and graft-versus-host disease, sustained CMV DNAemia and high CD8+ counts showed successive negative protein-based ELISpot results but a high and sustained response to IE-1 peptides. Our results suggest that the response to exogenous proteins, which involves their uptake and processing by antigen-presenting cells, more closely reflects the physiological response to CMV infection, while the response to exogenous peptides may lead to artificial in vitro T-cell responses, especially in strongly immunosuppressed patients.
U2 - 10.3390/diagnostics11020312
DO - 10.3390/diagnostics11020312
M3 - SCORING: Journal article
C2 - 33671952
VL - 11
JO - DIAGNOSTICS
JF - DIAGNOSTICS
SN - 2075-4418
IS - 2
M1 - 312
ER -