Cell cycle progression requires the CDC-48UFD-1/NPL-4 complex for efficient DNA replication.
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Cell cycle progression requires the CDC-48UFD-1/NPL-4 complex for efficient DNA replication. / Mouysset, Julien; Deichsel, Alexandra; Moser, Sandra; Hoege, Carsten; Hyman, Anthony A; Gartner, Anton; Hoppe, Thorsten.
in: P NATL ACAD SCI USA, Jahrgang 105, Nr. 35, 35, 2008, S. 12879-12884.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Cell cycle progression requires the CDC-48UFD-1/NPL-4 complex for efficient DNA replication.
AU - Mouysset, Julien
AU - Deichsel, Alexandra
AU - Moser, Sandra
AU - Hoege, Carsten
AU - Hyman, Anthony A
AU - Gartner, Anton
AU - Hoppe, Thorsten
PY - 2008
Y1 - 2008
N2 - Since cdc48 mutants were isolated by the first genetic screens for cell division cycle (cdc) mutants in yeast, the requirement of the chaperone-like ATPase Cdc48/p97 during cell division has remained unclear. Here, we discover an unanticipated function for Caenorhabditis elegans CDC-48 in DNA replication linked to cell cycle control. Our analysis of the CDC-48(UFD-1/NPL-4) complex identified a general role in S phase progression of mitotic cells essential for embryonic cell division and germline development of adult worms. These developmental defects result from activation of the DNA replication checkpoint caused by replication stress. Similar to loss of replication licensing factors, DNA content is strongly reduced in worms depleted for CDC-48, UFD-1, and NPL-4. In addition, these worms show decreased DNA synthesis and hypersensitivity toward replication blocking agents. Our findings identified a role for CDC-48(UFD-1/NPL-4) in DNA replication, which is important for cell cycle progression and genome stability.
AB - Since cdc48 mutants were isolated by the first genetic screens for cell division cycle (cdc) mutants in yeast, the requirement of the chaperone-like ATPase Cdc48/p97 during cell division has remained unclear. Here, we discover an unanticipated function for Caenorhabditis elegans CDC-48 in DNA replication linked to cell cycle control. Our analysis of the CDC-48(UFD-1/NPL-4) complex identified a general role in S phase progression of mitotic cells essential for embryonic cell division and germline development of adult worms. These developmental defects result from activation of the DNA replication checkpoint caused by replication stress. Similar to loss of replication licensing factors, DNA content is strongly reduced in worms depleted for CDC-48, UFD-1, and NPL-4. In addition, these worms show decreased DNA synthesis and hypersensitivity toward replication blocking agents. Our findings identified a role for CDC-48(UFD-1/NPL-4) in DNA replication, which is important for cell cycle progression and genome stability.
M3 - SCORING: Zeitschriftenaufsatz
VL - 105
SP - 12879
EP - 12884
JO - P NATL ACAD SCI USA
JF - P NATL ACAD SCI USA
SN - 0027-8424
IS - 35
M1 - 35
ER -