B-scan ultrasonographic monitoring of orthotopic xenografted plexiform neurofibroma in mice
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B-scan ultrasonographic monitoring of orthotopic xenografted plexiform neurofibroma in mice. / Gleiss, Jacob; Demestre, Maria; Spyra, Melanie; Eschenhagen, Thomas; Hagel, Christian; Mautner, Victor-Felix; Kluwe, Lan; Friedrich, Reinhard E.
in: IN VIVO, Jahrgang 27, Nr. 6, 2013, S. 723-7.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - B-scan ultrasonographic monitoring of orthotopic xenografted plexiform neurofibroma in mice
AU - Gleiss, Jacob
AU - Demestre, Maria
AU - Spyra, Melanie
AU - Eschenhagen, Thomas
AU - Hagel, Christian
AU - Mautner, Victor-Felix
AU - Kluwe, Lan
AU - Friedrich, Reinhard E
PY - 2013
Y1 - 2013
N2 - BACKGROUND/AIM: Xenografted benign tumours in immunodeficient mice provide an in vivo model to study tumour biology and the effect of agents on tumour growth. Conventionally, these small grafts can only be monitored upon sacrificing the animals. We evaluated ultrasound biomicroscopy for monitoring such grafts in vivo.MATERIALS AND METHODS: Small fragments (<10 mm(3)) of a plexiform neurofibroma obtained from patients with established diagnosis of neurofibromatosis type-1 (NF1) were orthotopically-xenografted onto the sciatic nerve of immunodeficient mice and monitored using a high-resolution in vivo micro-imaging system.RESULTS: Grafts were identified in most cases and were distinguished from the surrounding inflammatory host tissues by detailed ultrasonographic signals. Graft sizes could be calculated precisely from serial scan sections and monitored during the whole course of drug treatment.CONCLUSION: High frequency sonographic measurement is a superior non-invasive method for monitoring small grafts of slowly growing benign tumours in mice in vivo, e.g. plexiform neurofibroma, and is especially suitable for tracing the effects of drugs at multiple time-points, thus allowing a very cost-effective follow-up.
AB - BACKGROUND/AIM: Xenografted benign tumours in immunodeficient mice provide an in vivo model to study tumour biology and the effect of agents on tumour growth. Conventionally, these small grafts can only be monitored upon sacrificing the animals. We evaluated ultrasound biomicroscopy for monitoring such grafts in vivo.MATERIALS AND METHODS: Small fragments (<10 mm(3)) of a plexiform neurofibroma obtained from patients with established diagnosis of neurofibromatosis type-1 (NF1) were orthotopically-xenografted onto the sciatic nerve of immunodeficient mice and monitored using a high-resolution in vivo micro-imaging system.RESULTS: Grafts were identified in most cases and were distinguished from the surrounding inflammatory host tissues by detailed ultrasonographic signals. Graft sizes could be calculated precisely from serial scan sections and monitored during the whole course of drug treatment.CONCLUSION: High frequency sonographic measurement is a superior non-invasive method for monitoring small grafts of slowly growing benign tumours in mice in vivo, e.g. plexiform neurofibroma, and is especially suitable for tracing the effects of drugs at multiple time-points, thus allowing a very cost-effective follow-up.
M3 - SCORING: Journal article
C2 - 24292574
VL - 27
SP - 723
EP - 727
JO - IN VIVO
JF - IN VIVO
SN - 0258-851X
IS - 6
ER -