Anti-tau phospho-specific Ser262 antibody recognizes a variety of abnormal hyper-phosphorylated tau deposits in tauopathies including Pick bodies and argyrophilic grains
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Anti-tau phospho-specific Ser262 antibody recognizes a variety of abnormal hyper-phosphorylated tau deposits in tauopathies including Pick bodies and argyrophilic grains. / Ferrer, I; Barrachina, M; Puig, B; Puig Martorell, Berta.
in: ACTA NEUROPATHOL, Jahrgang 104, Nr. 6, 01.12.2002, S. 658-64.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - Anti-tau phospho-specific Ser262 antibody recognizes a variety of abnormal hyper-phosphorylated tau deposits in tauopathies including Pick bodies and argyrophilic grains
AU - Ferrer, I
AU - Barrachina, M
AU - Puig, B
AU - Puig Martorell, Berta
PY - 2002/12/1
Y1 - 2002/12/1
N2 - The rabbit polyclonal anti-tau phospho-specific Ser262 antibody (577814 Calbiochem) recognizes disease-specific band patterns on Western blots of sarkosyl-insoluble fractions in Alzheimer's disease (AD), progressive supranuclear palsy (PSP), corticobasal degeneration (CBD), argyrophilic grain disease (AGD) and Pick's disease (PiD): four bands of 74/72, 68, 64 and 60 kDa in AD, two bands of 68 and 64 kDa in PSP, CBD and AGD, and two bands of 64 and 60 kDa in PiD. Moreover, anti-tau phospho-specific Ser262 decorates neurons with neurofibrillary tangles, neurons with pre-tangles, dystrophic neurites of senile plaques, neuropil threads, Pick bodies, argyrophilic grains, and coiled bodies. Achromatic neurons in CBD, ballooned neurons in AGD, tufted astrocytes in PSP, astrocytic plaques in CBD and tau-containing astrocytes in AGD are not immunostained with the anti-tau phospho-specific Ser262 antibody. The lack of phospho-specific Ser262 immunoreactivity in tau-containing inclusions in astrocytes suggests different kinase equipment and activation in comparing neurons and astrocytes in tauopathies. Pick bodies in PiD and grains in AGD are weakly, or not all, immunostained in tissue samples with long post-mortem delays, although Ser262 is preserved in brain homogenates corresponding to the same time points processed for Western blot. This indicates postmortem modifications of tau in Pick bodies and argyrophilic grains, but not in other tau-containing inclusions, including paired helical filaments and coiled bodies, and suggests differences in tau conformation, particularly that involving phospho-tau Ser262 among tauopathies. However, it is important to note that phosphorylation of tau at Ser262 does occur in Pick bodies and argyrophilic grains, and this may have important consequences in reducing the capacity of binding phospho-tau to microtubules in these inclusions.
AB - The rabbit polyclonal anti-tau phospho-specific Ser262 antibody (577814 Calbiochem) recognizes disease-specific band patterns on Western blots of sarkosyl-insoluble fractions in Alzheimer's disease (AD), progressive supranuclear palsy (PSP), corticobasal degeneration (CBD), argyrophilic grain disease (AGD) and Pick's disease (PiD): four bands of 74/72, 68, 64 and 60 kDa in AD, two bands of 68 and 64 kDa in PSP, CBD and AGD, and two bands of 64 and 60 kDa in PiD. Moreover, anti-tau phospho-specific Ser262 decorates neurons with neurofibrillary tangles, neurons with pre-tangles, dystrophic neurites of senile plaques, neuropil threads, Pick bodies, argyrophilic grains, and coiled bodies. Achromatic neurons in CBD, ballooned neurons in AGD, tufted astrocytes in PSP, astrocytic plaques in CBD and tau-containing astrocytes in AGD are not immunostained with the anti-tau phospho-specific Ser262 antibody. The lack of phospho-specific Ser262 immunoreactivity in tau-containing inclusions in astrocytes suggests different kinase equipment and activation in comparing neurons and astrocytes in tauopathies. Pick bodies in PiD and grains in AGD are weakly, or not all, immunostained in tissue samples with long post-mortem delays, although Ser262 is preserved in brain homogenates corresponding to the same time points processed for Western blot. This indicates postmortem modifications of tau in Pick bodies and argyrophilic grains, but not in other tau-containing inclusions, including paired helical filaments and coiled bodies, and suggests differences in tau conformation, particularly that involving phospho-tau Ser262 among tauopathies. However, it is important to note that phosphorylation of tau at Ser262 does occur in Pick bodies and argyrophilic grains, and this may have important consequences in reducing the capacity of binding phospho-tau to microtubules in these inclusions.
KW - Aged
KW - Aged, 80 and over
KW - Antibodies
KW - Brain
KW - Diagnosis, Differential
KW - Female
KW - Humans
KW - Male
KW - Microtubules
KW - Neurodegenerative Diseases
KW - Phosphorylation
KW - Pick Disease of the Brain
KW - Tauopathies
KW - tau Proteins
U2 - 10.1007/s00401-002-0600-2
DO - 10.1007/s00401-002-0600-2
M3 - SCORING: Journal article
C2 - 12410387
VL - 104
SP - 658
EP - 664
JO - ACTA NEUROPATHOL
JF - ACTA NEUROPATHOL
SN - 0001-6322
IS - 6
ER -