Antisense ATM gene therapy: a strategy to increase the radiosensitivity of human tumors.
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Antisense ATM gene therapy: a strategy to increase the radiosensitivity of human tumors. / Guha, C; Guha, U; Tribius, Silke; Alfieri, A; Casper, D; Chakravarty, P; Mellado, W; Pandita, T K; Vikram, B.
in: GENE THER, Jahrgang 7, Nr. 10, 10, 2000, S. 852-858.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Antisense ATM gene therapy: a strategy to increase the radiosensitivity of human tumors.
AU - Guha, C
AU - Guha, U
AU - Tribius, Silke
AU - Alfieri, A
AU - Casper, D
AU - Chakravarty, P
AU - Mellado, W
AU - Pandita, T K
AU - Vikram, B
PY - 2000
Y1 - 2000
N2 - Atm, the gene mutated in ataxia-telangiectasia (AT) patients, is an essential component of the signal transduction pathway that responds to DNA damage due to ionizing radiation (IR). We attenuated ATM protein expression in human glioblastoma cells by expressing antisense RNA to a functional domain of the atm gene. While ATM expression decreased, constitutive expression of p53 and p21 increased. Irradiated ATM-attenuated cells failed to induce p53, demonstrated radioresistant DNA synthesis, and increased radiosensitivity. Antisense-ATM gene therapy in conjunction with radiation therapy may provide a novel strategy for the treatment of cancer.
AB - Atm, the gene mutated in ataxia-telangiectasia (AT) patients, is an essential component of the signal transduction pathway that responds to DNA damage due to ionizing radiation (IR). We attenuated ATM protein expression in human glioblastoma cells by expressing antisense RNA to a functional domain of the atm gene. While ATM expression decreased, constitutive expression of p53 and p21 increased. Irradiated ATM-attenuated cells failed to induce p53, demonstrated radioresistant DNA synthesis, and increased radiosensitivity. Antisense-ATM gene therapy in conjunction with radiation therapy may provide a novel strategy for the treatment of cancer.
M3 - SCORING: Zeitschriftenaufsatz
VL - 7
SP - 852
EP - 858
JO - GENE THER
JF - GENE THER
SN - 0969-7128
IS - 10
M1 - 10
ER -