An optimized protocol for a standardized, femoral osteotomy model to study fracture healing in mice
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An optimized protocol for a standardized, femoral osteotomy model to study fracture healing in mice. / Jiang, Shan; Knapstein, Paul; Donat, Antonia; Tsitsilonis, Serafeim; Keller, Johannes.
in: STAR PROTOC, Jahrgang 2, Nr. 3, 17.09.2021, S. 100798.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - An optimized protocol for a standardized, femoral osteotomy model to study fracture healing in mice
AU - Jiang, Shan
AU - Knapstein, Paul
AU - Donat, Antonia
AU - Tsitsilonis, Serafeim
AU - Keller, Johannes
N1 - © 2021 The Author(s).
PY - 2021/9/17
Y1 - 2021/9/17
N2 - Fracture healing represents a dynamic and complex process which depends on the balanced activities of a broad array of different cell types and signaling pathways. Here, we describe a femoral osteotomy protocol for mice, using an external fixator to stabilize the fractured bone. Depending on experimental requirements, the size of the osteotomy gap can be adjusted. Taken together, this protocol describes a highly standardized and reproducible murine model for morphologic and biomolecular assessment of the fracture healing process. For complete details on the use and execution of this protocol, please refer to Appelt et al. (2020).
AB - Fracture healing represents a dynamic and complex process which depends on the balanced activities of a broad array of different cell types and signaling pathways. Here, we describe a femoral osteotomy protocol for mice, using an external fixator to stabilize the fractured bone. Depending on experimental requirements, the size of the osteotomy gap can be adjusted. Taken together, this protocol describes a highly standardized and reproducible murine model for morphologic and biomolecular assessment of the fracture healing process. For complete details on the use and execution of this protocol, please refer to Appelt et al. (2020).
U2 - 10.1016/j.xpro.2021.100798
DO - 10.1016/j.xpro.2021.100798
M3 - SCORING: Journal article
C2 - 34527955
VL - 2
SP - 100798
JO - STAR PROTOC
JF - STAR PROTOC
SN - 2666-1667
IS - 3
ER -