Absence of H- and K-ras oncogene mutations in sporadic medullary thyroid carcinoma
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Absence of H- and K-ras oncogene mutations in sporadic medullary thyroid carcinoma. / Bockhorn, M; Frilling, A; Kalinin, V; Schröder, S; Broelsch, C E.
in: EXP CLIN ENDOCR DIAB, Jahrgang 108, Nr. 1, 01.01.2000, S. 49-53.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - Absence of H- and K-ras oncogene mutations in sporadic medullary thyroid carcinoma
AU - Bockhorn, M
AU - Frilling, A
AU - Kalinin, V
AU - Schröder, S
AU - Broelsch, C E
PY - 2000/1/1
Y1 - 2000/1/1
N2 - Medullary thyroid carcinoma (MTC) occurs sporadically (sMTC) or as part of the inherited cancer syndrome, multiple endocrine neoplasia type 2 (MEN 2). While the occurence of the MEN 2 syndrome is associated with mutations in the RET protooncogene, the reason for carcinogenesis in sMTC still remains unclear. Ras is a frequently mutated oncogene in a broad spectrum of human tumors and has been found in about 50% of follicular, papillary or anaplastic thyroid carcinomas. The purpose of this study was to determine, whether mutations in the ras oncogene could play a possible role in the carcinogenesis of sMTC. In this study we analyzed 15 sMTC for mutations in the hotspots codon 12, 13 and 61 of the H- and K-ras oncogene. We used the direct sequencing technique. In none of the examined tumors we were able to detect a mutation in the codon 12, 13 and 61 of the H-ras and K-ras oncogene. Based upon these results, we conclude that H- and K-ras do not play an important role in the carcinogenesis of sMTC.
AB - Medullary thyroid carcinoma (MTC) occurs sporadically (sMTC) or as part of the inherited cancer syndrome, multiple endocrine neoplasia type 2 (MEN 2). While the occurence of the MEN 2 syndrome is associated with mutations in the RET protooncogene, the reason for carcinogenesis in sMTC still remains unclear. Ras is a frequently mutated oncogene in a broad spectrum of human tumors and has been found in about 50% of follicular, papillary or anaplastic thyroid carcinomas. The purpose of this study was to determine, whether mutations in the ras oncogene could play a possible role in the carcinogenesis of sMTC. In this study we analyzed 15 sMTC for mutations in the hotspots codon 12, 13 and 61 of the H- and K-ras oncogene. We used the direct sequencing technique. In none of the examined tumors we were able to detect a mutation in the codon 12, 13 and 61 of the H-ras and K-ras oncogene. Based upon these results, we conclude that H- and K-ras do not play an important role in the carcinogenesis of sMTC.
KW - Base Sequence
KW - Carcinoma, Medullary
KW - Codon
KW - Genes, ras
KW - Humans
KW - Mutation
KW - Polymerase Chain Reaction
KW - Proto-Oncogene Proteins p21(ras)
KW - Thyroid Neoplasms
M3 - SCORING: Journal article
C2 - 10768832
VL - 108
SP - 49
EP - 53
JO - EXP CLIN ENDOCR DIAB
JF - EXP CLIN ENDOCR DIAB
SN - 0947-7349
IS - 1
ER -