A vasopressor factor partially purified from human parathyroid glands
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A vasopressor factor partially purified from human parathyroid glands. / Schlüter, H; Quante, C; Buchholz, B; Dietl, K H; Spieker, C; Karas, M; Zidek, W.
in: BIOCHEM BIOPH RES CO, Jahrgang 188, Nr. 1, 15.10.1992, S. 323-9.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - A vasopressor factor partially purified from human parathyroid glands
AU - Schlüter, H
AU - Quante, C
AU - Buchholz, B
AU - Dietl, K H
AU - Spieker, C
AU - Karas, M
AU - Zidek, W
PY - 1992/10/15
Y1 - 1992/10/15
N2 - Recently, a parathyroid hypertensive factor was postulated to play a role in the pathogenesis of hypertension in genetically hypertensive rats. Therefore it was examined, whether in human parathyroid glands a vasopressor substance can be detected. For this purpose, homogenates of hyperplastic parathyroid glands from 20 patients with tertiary hyperparathyroidism were deproteinized and fractionated by gel chromatography. The fractions obtained were tested for vasopressor activity in isolated perfused rat kidneys. A vasopressor fraction containing substances of 0.6-2.5 kDa was identified in the parathyroid glands. The responsible product was heat sensitive, peptidase-, trypsin- and carboxypeptidase y- sensitive and hydrophilic, as it did not bind to hydrophobic reversed-phase gel. These results suggest that parathyroid glands contain a hydrophilic peptide-like vasopressor substance different from the parathyroid hormone.
AB - Recently, a parathyroid hypertensive factor was postulated to play a role in the pathogenesis of hypertension in genetically hypertensive rats. Therefore it was examined, whether in human parathyroid glands a vasopressor substance can be detected. For this purpose, homogenates of hyperplastic parathyroid glands from 20 patients with tertiary hyperparathyroidism were deproteinized and fractionated by gel chromatography. The fractions obtained were tested for vasopressor activity in isolated perfused rat kidneys. A vasopressor fraction containing substances of 0.6-2.5 kDa was identified in the parathyroid glands. The responsible product was heat sensitive, peptidase-, trypsin- and carboxypeptidase y- sensitive and hydrophilic, as it did not bind to hydrophobic reversed-phase gel. These results suggest that parathyroid glands contain a hydrophilic peptide-like vasopressor substance different from the parathyroid hormone.
KW - Angiotensin II
KW - Animals
KW - Endopeptidases
KW - Humans
KW - Hyperparathyroidism
KW - Hypertension
KW - In Vitro Techniques
KW - Kidney
KW - Kidney Failure, Chronic
KW - Male
KW - Mass Spectrometry
KW - Muscle, Smooth, Vascular
KW - Parathyroid Glands
KW - Rats
KW - Rats, Inbred WKY
KW - Renal Artery
KW - Renal Circulation
KW - Tissue Extracts
KW - Vasoconstrictor Agents
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
M3 - SCORING: Journal article
C2 - 1417852
VL - 188
SP - 323
EP - 329
JO - BIOCHEM BIOPH RES CO
JF - BIOCHEM BIOPH RES CO
SN - 0006-291X
IS - 1
ER -