A novel workflow for three-dimensional analysis of tumour cell migration
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A novel workflow for three-dimensional analysis of tumour cell migration. / Ketchen, Sophie; Rohwedder, Arndt; Knipp, Sabine; Esteves, Filomena; Struve, Nina; Peckham, Michelle; Ladbury, John E; Curd, Alistair; Short, Susan C; Brüning-Richardson, Anke.
in: INTERFACE FOCUS, Jahrgang 10, Nr. 2, 06.04.2020, S. 20190070.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - A novel workflow for three-dimensional analysis of tumour cell migration
AU - Ketchen, Sophie
AU - Rohwedder, Arndt
AU - Knipp, Sabine
AU - Esteves, Filomena
AU - Struve, Nina
AU - Peckham, Michelle
AU - Ladbury, John E
AU - Curd, Alistair
AU - Short, Susan C
AU - Brüning-Richardson, Anke
N1 - © 2020 The Author(s).
PY - 2020/4/6
Y1 - 2020/4/6
N2 - The limitations of two-dimensional analysis in three-dimensional (3D) cellular imaging impair the accuracy of research findings in biological studies. Here, we report a novel 3D approach to acquisition, analysis and interpretation of tumour spheroid images. Our research interest in mesenchymal-amoeboid transition led to the development of a workflow incorporating the generation and analysis of 3D data with instant structured illumination microscopy and a new ImageJ plugin.
AB - The limitations of two-dimensional analysis in three-dimensional (3D) cellular imaging impair the accuracy of research findings in biological studies. Here, we report a novel 3D approach to acquisition, analysis and interpretation of tumour spheroid images. Our research interest in mesenchymal-amoeboid transition led to the development of a workflow incorporating the generation and analysis of 3D data with instant structured illumination microscopy and a new ImageJ plugin.
U2 - 10.1098/rsfs.2019.0070
DO - 10.1098/rsfs.2019.0070
M3 - SCORING: Journal article
C2 - 32194931
VL - 10
SP - 20190070
JO - INTERFACE FOCUS
JF - INTERFACE FOCUS
SN - 2042-8898
IS - 2
ER -