A Dendritic Golgi Satellite between ERGIC and Retromer
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A Dendritic Golgi Satellite between ERGIC and Retromer. / Mikhaylova, Marina; Bera, Sujoy; Kobler, Oliver; Frischknecht, Renato; Kreutz, Michael R .
in: CELL REP, Jahrgang 14, Nr. 2, 12.01.2016, S. 189-199.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - A Dendritic Golgi Satellite between ERGIC and Retromer
AU - Mikhaylova, Marina
AU - Bera, Sujoy
AU - Kobler, Oliver
AU - Frischknecht, Renato
AU - Kreutz, Michael R
PY - 2016/1/12
Y1 - 2016/1/12
N2 - The local synthesis of transmembrane proteins underlies functional specialization of dendritic microdomains in neuronal plasticity. It is unclear whether these proteins have access to the complete machinery of the secretory pathway following local synthesis. In this study, we describe a probe called pGolt that allows visualization of Golgi-related organelles for live imaging in neurons. We show that pGolt labels a widespread microsecretory Golgi satellite (GS) system that is, in contrast to Golgi outposts, present throughout basal and apical dendrites of all pyramidal neurons. The GS system contains glycosylation machinery and is localized between ERGIC and retromer. Synaptic activity restrains lateral movement of ERGIC, GS, and retromer close to one another, allowing confined processing of secretory cargo. Several synaptic transmembrane proteins pass through and recycle back to the GS system. Thus, the presence of an ER-ERGIC-GS-retromer microsecretory system in all neuronal dendrites enables autonomous local control of transmembrane protein synthesis and processing.
AB - The local synthesis of transmembrane proteins underlies functional specialization of dendritic microdomains in neuronal plasticity. It is unclear whether these proteins have access to the complete machinery of the secretory pathway following local synthesis. In this study, we describe a probe called pGolt that allows visualization of Golgi-related organelles for live imaging in neurons. We show that pGolt labels a widespread microsecretory Golgi satellite (GS) system that is, in contrast to Golgi outposts, present throughout basal and apical dendrites of all pyramidal neurons. The GS system contains glycosylation machinery and is localized between ERGIC and retromer. Synaptic activity restrains lateral movement of ERGIC, GS, and retromer close to one another, allowing confined processing of secretory cargo. Several synaptic transmembrane proteins pass through and recycle back to the GS system. Thus, the presence of an ER-ERGIC-GS-retromer microsecretory system in all neuronal dendrites enables autonomous local control of transmembrane protein synthesis and processing.
U2 - 10.1016/j.celrep.2015.12.024
DO - 10.1016/j.celrep.2015.12.024
M3 - SCORING: Journal article
VL - 14
SP - 189
EP - 199
JO - CELL REP
JF - CELL REP
SN - 2211-1247
IS - 2
ER -