WT1 protein expression in childhood acute leukemia
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WT1 protein expression in childhood acute leukemia. / Kerst, Gunter; Bergold, Nina; Gieseke, Friederike; Coustan-Smith, Elaine; Lang, Peter; Kalinova, Marketa; Handgretinger, Rupert; Trka, Jan; Müller, Ingo.
In: AM J HEMATOL, Vol. 83, No. 5, 05.2008, p. 382-6.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - WT1 protein expression in childhood acute leukemia
AU - Kerst, Gunter
AU - Bergold, Nina
AU - Gieseke, Friederike
AU - Coustan-Smith, Elaine
AU - Lang, Peter
AU - Kalinova, Marketa
AU - Handgretinger, Rupert
AU - Trka, Jan
AU - Müller, Ingo
N1 - Copyright 2008 Wiley-Liss, Inc.
PY - 2008/5
Y1 - 2008/5
N2 - In patients with acute leukemia, Wilms' tumor gene 1 (WT1) has been used as a target for the detection of minimal residual disease (MRD) by PCR techniques. The expression of WT1 protein, however, has not been extensively studied. To determine the relation between expression of WT1 transcripts and of the encoded protein, we examined leukemic cell lines and primary childhood leukemia samples using both real-time quantitative PCR (RQ-PCR) and flow cytometry. WT1 protein was highly expressed in the leukemic cell lines K562, HL-60, PLB 985, KG-1a and CEM. By contrast, 40 primary samples of acute lymphoblastic leukemia (ALL; B-ALL, n = 15 and T-ALL, n = 10) and acute myeloid leukemia (n = 15) expressed low levels of WT1 protein. RQ-PCR detected WT1 transcript levels in the same range as reported in earlier studies in childhood acute leukemia. The results of this study indicate the following: (i) there are considerable discrepancies between WT1 transcripts and protein expression; (ii) WT1 is not a suitable marker for flow cytometric MRD detection in childhood acute leukemia.
AB - In patients with acute leukemia, Wilms' tumor gene 1 (WT1) has been used as a target for the detection of minimal residual disease (MRD) by PCR techniques. The expression of WT1 protein, however, has not been extensively studied. To determine the relation between expression of WT1 transcripts and of the encoded protein, we examined leukemic cell lines and primary childhood leukemia samples using both real-time quantitative PCR (RQ-PCR) and flow cytometry. WT1 protein was highly expressed in the leukemic cell lines K562, HL-60, PLB 985, KG-1a and CEM. By contrast, 40 primary samples of acute lymphoblastic leukemia (ALL; B-ALL, n = 15 and T-ALL, n = 10) and acute myeloid leukemia (n = 15) expressed low levels of WT1 protein. RQ-PCR detected WT1 transcript levels in the same range as reported in earlier studies in childhood acute leukemia. The results of this study indicate the following: (i) there are considerable discrepancies between WT1 transcripts and protein expression; (ii) WT1 is not a suitable marker for flow cytometric MRD detection in childhood acute leukemia.
KW - Adolescent
KW - Adult
KW - Biomarkers, Tumor
KW - Blood Cells
KW - Bone Marrow Cells
KW - Burkitt Lymphoma
KW - Cell Line, Tumor
KW - Child
KW - Child, Preschool
KW - Female
KW - Flow Cytometry
KW - Gene Expression Regulation, Leukemic
KW - Genes, Wilms Tumor
KW - Humans
KW - Leukemia, Myeloid, Acute
KW - Leukemia-Lymphoma, Adult T-Cell
KW - Male
KW - Neoplasm Proteins
KW - Precursor Cell Lymphoblastic Leukemia-Lymphoma
KW - RNA, Messenger
KW - RNA, Neoplasm
KW - Sensitivity and Specificity
KW - WT1 Proteins
U2 - 10.1002/ajh.21123
DO - 10.1002/ajh.21123
M3 - SCORING: Journal article
C2 - 18161786
VL - 83
SP - 382
EP - 386
JO - AM J HEMATOL
JF - AM J HEMATOL
SN - 0361-8609
IS - 5
ER -
