Wound-induced apoplastic peroxidase activities: their roles in the production and detoxification of reactive oxygen species.
Standard
Wound-induced apoplastic peroxidase activities: their roles in the production and detoxification of reactive oxygen species. / Minibayeva, F; Kolesnikov, O; Chasov, A; Beckett, R P; Lüthje, S; Vylegzhanina, N; Buck, Friedrich; Böttger, M.
In: PLANT CELL ENVIRON, Vol. 32, No. 5, 5, 2009, p. 497-508.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Wound-induced apoplastic peroxidase activities: their roles in the production and detoxification of reactive oxygen species.
AU - Minibayeva, F
AU - Kolesnikov, O
AU - Chasov, A
AU - Beckett, R P
AU - Lüthje, S
AU - Vylegzhanina, N
AU - Buck, Friedrich
AU - Böttger, M
PY - 2009
Y1 - 2009
N2 - Production of reactive oxygen species (ROS) is a widely reported response of plants to wounding. However, the nature of enzymes responsible for ROS production and metabolism in the apoplast is still an open question. We identified and characterized the proteins responsible for the wound-induced production and detoxification of ROS in the apoplast of wheat roots (Triticum aestivum L.). Compared to intact roots, excised roots and leachates derived from them produced twice the amount of superoxide (O2(*-)). Wounding also induced extracellular peroxidase (ECPOX) activity mainly caused by the release of soluble peroxidases with molecular masses of 37, 40 and 136 kD. Peptide mass analysis by electrospray ionization-quadrupole time-of-flight-tandem mass spectrometry (ESI-QTOF-MS/MS) following lectin affinity chromatography of leachates showed the presence of peroxidases in unbound (37 kD) and bound (40 kD) fractions. High sensitivity of O2(*-)-producing activity to peroxidase inhibitors and production of O2(*-) by purified peroxidases in vitro provided evidence for the involvement of ECPOXs in O2(*-) production in the apoplast. Our results present new insights into the rapid response of roots to wounding. An important component of this response is mediated by peroxidases that are released from the cell surface into the apoplast where they can display both oxidative and peroxidative activities.
AB - Production of reactive oxygen species (ROS) is a widely reported response of plants to wounding. However, the nature of enzymes responsible for ROS production and metabolism in the apoplast is still an open question. We identified and characterized the proteins responsible for the wound-induced production and detoxification of ROS in the apoplast of wheat roots (Triticum aestivum L.). Compared to intact roots, excised roots and leachates derived from them produced twice the amount of superoxide (O2(*-)). Wounding also induced extracellular peroxidase (ECPOX) activity mainly caused by the release of soluble peroxidases with molecular masses of 37, 40 and 136 kD. Peptide mass analysis by electrospray ionization-quadrupole time-of-flight-tandem mass spectrometry (ESI-QTOF-MS/MS) following lectin affinity chromatography of leachates showed the presence of peroxidases in unbound (37 kD) and bound (40 kD) fractions. High sensitivity of O2(*-)-producing activity to peroxidase inhibitors and production of O2(*-) by purified peroxidases in vitro provided evidence for the involvement of ECPOXs in O2(*-) production in the apoplast. Our results present new insights into the rapid response of roots to wounding. An important component of this response is mediated by peroxidases that are released from the cell surface into the apoplast where they can display both oxidative and peroxidative activities.
M3 - SCORING: Zeitschriftenaufsatz
VL - 32
SP - 497
EP - 508
JO - PLANT CELL ENVIRON
JF - PLANT CELL ENVIRON
SN - 0140-7791
IS - 5
M1 - 5
ER -
