WNT5A is induced by inflammatory mediators in bone marrow stromal cells and regulates cytokine and chemokine production.

TY - JOUR

T1 - WNT5A is induced by inflammatory mediators in bone marrow stromal cells and regulates cytokine and chemokine production.

AU - Rauner, Martina

AU - Stein, Nicola

AU - Winzer, Maria

AU - Goettsch, Claudia

AU - Zwerina, Jochen

AU - Schett, Georg

AU - Distler, Jörg H W

AU - Albers, Joachim

AU - Schulze, Jochen

AU - Schinke, Thorsten

AU - Bornhäuser, Martin

AU - Platzbecker, Uwe

AU - Hofbauer, Lorenz C

PY - 2012

Y1 - 2012

N2 - WNT5A has recently been implicated in inflammatory processes, but its role as a bone marrow stromal cell (BMSC)-derived mediator of joint inflammation in arthritis is unclear. Here, we investigated whether inflammatory stimuli induce WNT5A in BMSC to control inflammatory responses. WNT5A levels were determined in human BMSC after stimulation with lipopolysaccharide (LPS) or tumor necrosis factor ? (TNF-?,) and in synovial cells and tissue of patients with rheumatoid arthritis (RA) and human TNF-? transgenic (hTNFtg) mice. A microarray analysis of WNT5A-treated murine osteoblasts was performed using Affymetrix gene chips. The regulation of cytokine/chemokine expression was confirmed by qPCR, ELISA, and Luminex technology in BMSC after stimulation with WNT5A or WNT5A knockdown. Relevant signaling pathways were identified using specific inhibitors. Migration of MACS-purified T lymphocytes and monocytes was assessed using the FluoroBlok system. WNT5A expression was increased threefold in BMSC after stimulation with LPS or TNF-?. Synovial fibroblasts from patients with RA showed a twofold increase of WNT5A expression compared with control cells, and its expression was highly induced in the synovial tissue of patients with RA and hTNFtg mice. Microarray analysis of WNT5A-treated osteoblasts identified cytokines and chemokines as targets. The induction of IL-1?, IL-6, CCL2, CCL5, CXCL1, and CXCL5 by WNT5A was confirmed in BMSC and depended on the activation of the NF-?B, mitogen-activated protein (MAPK), and Akt pathways. Accordingly, knockdown of WNT5A markedly reduced the basal and LPS-induced cytokine/chemokine production. Finally, migration of monocytes and T cells toward the supernatant of WNT5A-treated BMSC was increased by 25% and 20%, respectively. This study underlines the critical role of BMSC-derived WNT5A in the regulation of inflammatory processes and suggests its participation in the pathogenesis of RA.

AB - WNT5A has recently been implicated in inflammatory processes, but its role as a bone marrow stromal cell (BMSC)-derived mediator of joint inflammation in arthritis is unclear. Here, we investigated whether inflammatory stimuli induce WNT5A in BMSC to control inflammatory responses. WNT5A levels were determined in human BMSC after stimulation with lipopolysaccharide (LPS) or tumor necrosis factor ? (TNF-?,) and in synovial cells and tissue of patients with rheumatoid arthritis (RA) and human TNF-? transgenic (hTNFtg) mice. A microarray analysis of WNT5A-treated murine osteoblasts was performed using Affymetrix gene chips. The regulation of cytokine/chemokine expression was confirmed by qPCR, ELISA, and Luminex technology in BMSC after stimulation with WNT5A or WNT5A knockdown. Relevant signaling pathways were identified using specific inhibitors. Migration of MACS-purified T lymphocytes and monocytes was assessed using the FluoroBlok system. WNT5A expression was increased threefold in BMSC after stimulation with LPS or TNF-?. Synovial fibroblasts from patients with RA showed a twofold increase of WNT5A expression compared with control cells, and its expression was highly induced in the synovial tissue of patients with RA and hTNFtg mice. Microarray analysis of WNT5A-treated osteoblasts identified cytokines and chemokines as targets. The induction of IL-1?, IL-6, CCL2, CCL5, CXCL1, and CXCL5 by WNT5A was confirmed in BMSC and depended on the activation of the NF-?B, mitogen-activated protein (MAPK), and Akt pathways. Accordingly, knockdown of WNT5A markedly reduced the basal and LPS-induced cytokine/chemokine production. Finally, migration of monocytes and T cells toward the supernatant of WNT5A-treated BMSC was increased by 25% and 20%, respectively. This study underlines the critical role of BMSC-derived WNT5A in the regulation of inflammatory processes and suggests its participation in the pathogenesis of RA.

KW - Adult

KW - Animals

KW - Humans

KW - Male

KW - Female

KW - Middle Aged

KW - Cells, Cultured

KW - Mice

KW - Mice, Inbred C57BL

KW - Cell Movement

KW - Cell Separation

KW - Cytokines/biosynthesis

KW - Lipopolysaccharides/pharmacology

KW - Tumor Necrosis Factor-alpha/pharmacology

KW - Arthritis, Rheumatoid/metabolism/pathology

KW - Bone Marrow Cells/drug effects/metabolism

KW - Chemokines/biosynthesis

KW - Inflammation Mediators/physiology

KW - Proto-Oncogene Proteins/biosynthesis

KW - Stromal Cells/drug effects/metabolism

KW - Wnt Proteins/biosynthesis

KW - Adult

KW - Animals

KW - Humans

KW - Male

KW - Female

KW - Middle Aged

KW - Cells, Cultured

KW - Mice

KW - Mice, Inbred C57BL

KW - Cell Movement

KW - Cell Separation

KW - Cytokines/biosynthesis

KW - Lipopolysaccharides/pharmacology

KW - Tumor Necrosis Factor-alpha/pharmacology

KW - Arthritis, Rheumatoid/metabolism/pathology

KW - Bone Marrow Cells/drug effects/metabolism

KW - Chemokines/biosynthesis

KW - Inflammation Mediators/physiology

KW - Proto-Oncogene Proteins/biosynthesis

KW - Stromal Cells/drug effects/metabolism

KW - Wnt Proteins/biosynthesis

M3 - SCORING: Journal article

VL - 27

SP - 575

EP - 585

JO - J BONE MINER RES

JF - J BONE MINER RES

SN - 0884-0431

IS - 3

M1 - 3

ER -