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wnt16 regulates spine and muscle morphogenesis through parallel signals from notochord and dermomyotome

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wnt16 regulates spine and muscle morphogenesis through parallel signals from notochord and dermomyotome. / Watson, Claire J; Tang, W Joyce; Rojas, Maria F; Fiedler, Imke A K; Morfin Montes de Oca, Ernesto; Cronrath, Andrea R; Callies, Lulu K; Swearer, Avery Angell; Ahmed, Ali R; Sethuraman, Visali; Addish, Sumaya; Farr, Gist H; Gómez, Arianna Ericka; Rai, Jyoti; Monstad-Rios, Adrian T; Gardiner, Edith M; Karasik, David; Maves, Lisa; Busse, Bjorn; Hsu, Yi-Hsiang; Kwon, Ronald Young.

In: PLOS GENET, Vol. 18, No. 11, e1010496, 11.2022.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Watson, CJ, Tang, WJ, Rojas, MF, Fiedler, IAK, Morfin Montes de Oca, E, Cronrath, AR, Callies, LK, Swearer, AA, Ahmed, AR, Sethuraman, V, Addish, S, Farr, GH, Gómez, AE, Rai, J, Monstad-Rios, AT, Gardiner, EM, Karasik, D, Maves, L, Busse, B, Hsu, Y-H & Kwon, RY 2022, 'wnt16 regulates spine and muscle morphogenesis through parallel signals from notochord and dermomyotome', PLOS GENET, vol. 18, no. 11, e1010496. https://doi.org/10.1371/journal.pgen.1010496

APA

Watson, C. J., Tang, W. J., Rojas, M. F., Fiedler, I. A. K., Morfin Montes de Oca, E., Cronrath, A. R., Callies, L. K., Swearer, A. A., Ahmed, A. R., Sethuraman, V., Addish, S., Farr, G. H., Gómez, A. E., Rai, J., Monstad-Rios, A. T., Gardiner, E. M., Karasik, D., Maves, L., Busse, B., ... Kwon, R. Y. (2022). wnt16 regulates spine and muscle morphogenesis through parallel signals from notochord and dermomyotome. PLOS GENET, 18(11), [e1010496]. https://doi.org/10.1371/journal.pgen.1010496

Vancouver

Watson CJ, Tang WJ, Rojas MF, Fiedler IAK, Morfin Montes de Oca E, Cronrath AR et al. wnt16 regulates spine and muscle morphogenesis through parallel signals from notochord and dermomyotome. PLOS GENET. 2022 Nov;18(11). e1010496. https://doi.org/10.1371/journal.pgen.1010496

Bibtex

@article{6e746d6b269741d2ae10b60762c29c89,
title = "wnt16 regulates spine and muscle morphogenesis through parallel signals from notochord and dermomyotome",
abstract = "Bone and muscle are coupled through developmental, mechanical, paracrine, and autocrine signals. Genetic variants at the CPED1-WNT16 locus are dually associated with bone- and muscle-related traits. While Wnt16 is necessary for bone mass and strength, this fails to explain pleiotropy at this locus. Here, we show wnt16 is required for spine and muscle morphogenesis in zebrafish. In embryos, wnt16 is expressed in dermomyotome and developing notochord, and contributes to larval myotome morphology and notochord elongation. Later, wnt16 is expressed at the ventral midline of the notochord sheath, and contributes to spine mineralization and osteoblast recruitment. Morphological changes in wnt16 mutant larvae are mirrored in adults, indicating that wnt16 impacts bone and muscle morphology throughout the lifespan. Finally, we show that wnt16 is a gene of major effect on lean mass at the CPED1-WNT16 locus. Our findings indicate that Wnt16 is secreted in structures adjacent to developing bone (notochord) and muscle (dermomyotome) where it affects the morphogenesis of each tissue, thereby rendering wnt16 expression into dual effects on bone and muscle morphology. This work expands our understanding of wnt16 in musculoskeletal development and supports the potential for variants to act through WNT16 to influence bone and muscle via parallel morphogenetic processes.",
keywords = "Animals, Notochord, Zebrafish/genetics, Spine, Muscles, Morphogenesis/genetics, Larva, Zebrafish Proteins/genetics, Wnt Proteins/genetics",
author = "Watson, {Claire J} and Tang, {W Joyce} and Rojas, {Maria F} and Fiedler, {Imke A K} and {Morfin Montes de Oca}, Ernesto and Cronrath, {Andrea R} and Callies, {Lulu K} and Swearer, {Avery Angell} and Ahmed, {Ali R} and Visali Sethuraman and Sumaya Addish and Farr, {Gist H} and G{\'o}mez, {Arianna Ericka} and Jyoti Rai and Monstad-Rios, {Adrian T} and Gardiner, {Edith M} and David Karasik and Lisa Maves and Bjorn Busse and Yi-Hsiang Hsu and Kwon, {Ronald Young}",
note = "Copyright: {\textcopyright} 2022 Watson et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.",
year = "2022",
month = nov,
doi = "10.1371/journal.pgen.1010496",
language = "English",
volume = "18",
journal = "PLOS GENET",
issn = "1553-7404",
publisher = "Public Library of Science",
number = "11",

}

RIS

TY - JOUR

T1 - wnt16 regulates spine and muscle morphogenesis through parallel signals from notochord and dermomyotome

AU - Watson, Claire J

AU - Tang, W Joyce

AU - Rojas, Maria F

AU - Fiedler, Imke A K

AU - Morfin Montes de Oca, Ernesto

AU - Cronrath, Andrea R

AU - Callies, Lulu K

AU - Swearer, Avery Angell

AU - Ahmed, Ali R

AU - Sethuraman, Visali

AU - Addish, Sumaya

AU - Farr, Gist H

AU - Gómez, Arianna Ericka

AU - Rai, Jyoti

AU - Monstad-Rios, Adrian T

AU - Gardiner, Edith M

AU - Karasik, David

AU - Maves, Lisa

AU - Busse, Bjorn

AU - Hsu, Yi-Hsiang

AU - Kwon, Ronald Young

N1 - Copyright: © 2022 Watson et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PY - 2022/11

Y1 - 2022/11

N2 - Bone and muscle are coupled through developmental, mechanical, paracrine, and autocrine signals. Genetic variants at the CPED1-WNT16 locus are dually associated with bone- and muscle-related traits. While Wnt16 is necessary for bone mass and strength, this fails to explain pleiotropy at this locus. Here, we show wnt16 is required for spine and muscle morphogenesis in zebrafish. In embryos, wnt16 is expressed in dermomyotome and developing notochord, and contributes to larval myotome morphology and notochord elongation. Later, wnt16 is expressed at the ventral midline of the notochord sheath, and contributes to spine mineralization and osteoblast recruitment. Morphological changes in wnt16 mutant larvae are mirrored in adults, indicating that wnt16 impacts bone and muscle morphology throughout the lifespan. Finally, we show that wnt16 is a gene of major effect on lean mass at the CPED1-WNT16 locus. Our findings indicate that Wnt16 is secreted in structures adjacent to developing bone (notochord) and muscle (dermomyotome) where it affects the morphogenesis of each tissue, thereby rendering wnt16 expression into dual effects on bone and muscle morphology. This work expands our understanding of wnt16 in musculoskeletal development and supports the potential for variants to act through WNT16 to influence bone and muscle via parallel morphogenetic processes.

AB - Bone and muscle are coupled through developmental, mechanical, paracrine, and autocrine signals. Genetic variants at the CPED1-WNT16 locus are dually associated with bone- and muscle-related traits. While Wnt16 is necessary for bone mass and strength, this fails to explain pleiotropy at this locus. Here, we show wnt16 is required for spine and muscle morphogenesis in zebrafish. In embryos, wnt16 is expressed in dermomyotome and developing notochord, and contributes to larval myotome morphology and notochord elongation. Later, wnt16 is expressed at the ventral midline of the notochord sheath, and contributes to spine mineralization and osteoblast recruitment. Morphological changes in wnt16 mutant larvae are mirrored in adults, indicating that wnt16 impacts bone and muscle morphology throughout the lifespan. Finally, we show that wnt16 is a gene of major effect on lean mass at the CPED1-WNT16 locus. Our findings indicate that Wnt16 is secreted in structures adjacent to developing bone (notochord) and muscle (dermomyotome) where it affects the morphogenesis of each tissue, thereby rendering wnt16 expression into dual effects on bone and muscle morphology. This work expands our understanding of wnt16 in musculoskeletal development and supports the potential for variants to act through WNT16 to influence bone and muscle via parallel morphogenetic processes.

KW - Animals

KW - Notochord

KW - Zebrafish/genetics

KW - Spine

KW - Muscles

KW - Morphogenesis/genetics

KW - Larva

KW - Zebrafish Proteins/genetics

KW - Wnt Proteins/genetics

U2 - 10.1371/journal.pgen.1010496

DO - 10.1371/journal.pgen.1010496

M3 - SCORING: Journal article

C2 - 36346812

VL - 18

JO - PLOS GENET

JF - PLOS GENET

SN - 1553-7404

IS - 11

M1 - e1010496

ER -