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Visual function restoration with a highly sensitive and fast Channelrhodopsin in blind mice

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Visual function restoration with a highly sensitive and fast Channelrhodopsin in blind mice. / Chen, Fei; Duan, Xiaodong; Yu, Yao; Yang, Shang; Chen, Yuanyuan; Gee, Christine E; Nagel, Georg; Zhang, Kang; Gao, Shiqiang; Shen, Yin.

In: SIGNAL TRANSDUCT TAR, Vol. 7, No. 1, 104, 18.04.2022.

Research output: SCORING: Contribution to journal › Letter › Research › peer-review

Harvard

Chen, F, Duan, X, Yu, Y, Yang, S, Chen, Y, Gee, CE, Nagel, G, Zhang, K, Gao, S & Shen, Y 2022, 'Visual function restoration with a highly sensitive and fast Channelrhodopsin in blind mice', SIGNAL TRANSDUCT TAR, vol. 7, no. 1, 104. https://doi.org/10.1038/s41392-022-00935-x

APA

Chen, F., Duan, X., Yu, Y., Yang, S., Chen, Y., Gee, C. E., Nagel, G., Zhang, K., Gao, S., & Shen, Y. (2022). Visual function restoration with a highly sensitive and fast Channelrhodopsin in blind mice. SIGNAL TRANSDUCT TAR, 7(1), [104]. https://doi.org/10.1038/s41392-022-00935-x

Vancouver

Chen F, Duan X, Yu Y, Yang S, Chen Y, Gee CE et al. Visual function restoration with a highly sensitive and fast Channelrhodopsin in blind mice. SIGNAL TRANSDUCT TAR. 2022 Apr 18;7(1). 104. https://doi.org/10.1038/s41392-022-00935-x

Bibtex

@article{2aa88d35279c490ead0dc039658eb0c8,
title = "Visual function restoration with a highly sensitive and fast Channelrhodopsin in blind mice",
abstract = "Inherited and age-related retinal degenerative diseases cause progressive loss of photoreceptors, ultimately leading to blindness. Optogenetics is a promising strategy for restoring visual function through photosensitive proteins{\textquoteright} ectopic expression in surviving retinal neurons. Very recently, the optogenetic method with a red-shifted Channelrhodopsin was clinically applied for partial recovery of visual function in a blind patient. However, major obstacles to achieving optimal optogenetic vision restoration are either the low light sensitivity or the slow kinetics of existing rhodopsin-based optogenetic tools, which can be improved by molecular engineering to enhance the efficacy of fast Channelrhodopsins (ChRs). Here, we present a newly engineered ChR variant PsCatCh2.0, engineered from PsChR, which displays inherently high Ca2+ and Na+ conductance and fast kinetics.",
author = "Fei Chen and Xiaodong Duan and Yao Yu and Shang Yang and Yuanyuan Chen and Gee, {Christine E} and Georg Nagel and Kang Zhang and Shiqiang Gao and Yin Shen",
year = "2022",
month = apr,
day = "18",
doi = "10.1038/s41392-022-00935-x",
language = "English",
volume = "7",
journal = "SIGNAL TRANSDUCT TAR",
issn = "2095-9907",
publisher = "Springer Nature",
number = "1",

}

RIS

TY - JOUR

T1 - Visual function restoration with a highly sensitive and fast Channelrhodopsin in blind mice

AU - Chen, Fei

AU - Duan, Xiaodong

AU - Yu, Yao

AU - Yang, Shang

AU - Chen, Yuanyuan

AU - Gee, Christine E

AU - Nagel, Georg

AU - Zhang, Kang

AU - Gao, Shiqiang

AU - Shen, Yin

PY - 2022/4/18

Y1 - 2022/4/18

N2 - Inherited and age-related retinal degenerative diseases cause progressive loss of photoreceptors, ultimately leading to blindness. Optogenetics is a promising strategy for restoring visual function through photosensitive proteins’ ectopic expression in surviving retinal neurons. Very recently, the optogenetic method with a red-shifted Channelrhodopsin was clinically applied for partial recovery of visual function in a blind patient. However, major obstacles to achieving optimal optogenetic vision restoration are either the low light sensitivity or the slow kinetics of existing rhodopsin-based optogenetic tools, which can be improved by molecular engineering to enhance the efficacy of fast Channelrhodopsins (ChRs). Here, we present a newly engineered ChR variant PsCatCh2.0, engineered from PsChR, which displays inherently high Ca2+ and Na+ conductance and fast kinetics.

AB - Inherited and age-related retinal degenerative diseases cause progressive loss of photoreceptors, ultimately leading to blindness. Optogenetics is a promising strategy for restoring visual function through photosensitive proteins’ ectopic expression in surviving retinal neurons. Very recently, the optogenetic method with a red-shifted Channelrhodopsin was clinically applied for partial recovery of visual function in a blind patient. However, major obstacles to achieving optimal optogenetic vision restoration are either the low light sensitivity or the slow kinetics of existing rhodopsin-based optogenetic tools, which can be improved by molecular engineering to enhance the efficacy of fast Channelrhodopsins (ChRs). Here, we present a newly engineered ChR variant PsCatCh2.0, engineered from PsChR, which displays inherently high Ca2+ and Na+ conductance and fast kinetics.

U2 - 10.1038/s41392-022-00935-x

DO - 10.1038/s41392-022-00935-x

M3 - Letter

C2 - 35430811

VL - 7

JO - SIGNAL TRANSDUCT TAR

JF - SIGNAL TRANSDUCT TAR

SN - 2095-9907

IS - 1

M1 - 104

ER -