Viral Vector-Based Transduction of Slice Cultures

J Simon Wiegert; Christine E Gee; Thomas G Oertner

doi:10.1101/pdb.prot094896

Viral Vector-Based Transduction of Slice Cultures

Standard

Viral Vector-Based Transduction of Slice Cultures. / Wiegert, J Simon; Gee, Christine E ; Oertner, Thomas G.

In: Cold Spring Harbor protocols, Vol. 2017, No. 2, 01.02.2017, p. pdb.prot094896.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Wiegert, JS, Gee, CE & Oertner, TG 2017, 'Viral Vector-Based Transduction of Slice Cultures', Cold Spring Harbor protocols, vol. 2017, no. 2, pp. pdb.prot094896. https://doi.org/10.1101/pdb.prot094896

APA

Wiegert, J. S., Gee, C. E., & Oertner, T. G. (2017). Viral Vector-Based Transduction of Slice Cultures. Cold Spring Harbor protocols, 2017(2), pdb.prot094896. https://doi.org/10.1101/pdb.prot094896

Vancouver

Wiegert JS, Gee CE , Oertner TG. Viral Vector-Based Transduction of Slice Cultures. Cold Spring Harbor protocols. 2017 Feb 1;2017(2):pdb.prot094896. https://doi.org/10.1101/pdb.prot094896

Bibtex

@article{33dc4e51676d47d481cc2c2e7fbae805,

title = "Viral Vector-Based Transduction of Slice Cultures",

abstract = "Transgenes can be introduced into the cells of organotypic slice cultures using different delivery methods, such as biolistic transfection, electroporation, and viral vector-based transduction. These methods produce different patterns of transgene expression. Local injection of recombinant adeno-associated virus (rAAV) produces a small cluster of transgene-expressing neurons around the injection site. Expression in individual cells varies with the distance from the injection site, indicating that many neurons take up several rAAV particles. The serotype and promoter also play a role in transgene expression. Here, we present a protocol for the transduction of previously prepared hippocampal slice cultures with rAVV.",

author = "Wiegert, {J Simon} and Gee, {Christine E} and Oertner, {Thomas G}",

note = "{\textcopyright} 2017 Cold Spring Harbor Laboratory Press.",

year = "2017",

month = feb,

day = "1",

doi = "10.1101/pdb.prot094896",

language = "English",

volume = "2017",

pages = "pdb.prot094896",

journal = "Cold Spring Harbor protocols",

issn = "1559-6095",

publisher = "Cold Spring Harbor Laboratory Press",

number = "2",

}

RIS

TY - JOUR

T1 - Viral Vector-Based Transduction of Slice Cultures

AU - Wiegert, J Simon

AU - Gee, Christine E

AU - Oertner, Thomas G

PY - 2017/2/1

Y1 - 2017/2/1

N2 - Transgenes can be introduced into the cells of organotypic slice cultures using different delivery methods, such as biolistic transfection, electroporation, and viral vector-based transduction. These methods produce different patterns of transgene expression. Local injection of recombinant adeno-associated virus (rAAV) produces a small cluster of transgene-expressing neurons around the injection site. Expression in individual cells varies with the distance from the injection site, indicating that many neurons take up several rAAV particles. The serotype and promoter also play a role in transgene expression. Here, we present a protocol for the transduction of previously prepared hippocampal slice cultures with rAVV.

AB - Transgenes can be introduced into the cells of organotypic slice cultures using different delivery methods, such as biolistic transfection, electroporation, and viral vector-based transduction. These methods produce different patterns of transgene expression. Local injection of recombinant adeno-associated virus (rAAV) produces a small cluster of transgene-expressing neurons around the injection site. Expression in individual cells varies with the distance from the injection site, indicating that many neurons take up several rAAV particles. The serotype and promoter also play a role in transgene expression. Here, we present a protocol for the transduction of previously prepared hippocampal slice cultures with rAVV.

U2 - 10.1101/pdb.prot094896

DO - 10.1101/pdb.prot094896

M3 - SCORING: Journal article

C2 - 28148855

VL - 2017

SP - pdb.prot094896

JO - Cold Spring Harbor protocols

JF - Cold Spring Harbor protocols

SN - 1559-6095

IS - 2

ER -