Very low prevalence of Mansonella perstans-specific cell-free DNA in serum samples of Ghanaian HIV patients
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Very low prevalence of Mansonella perstans-specific cell-free DNA in serum samples of Ghanaian HIV patients. / Eberhardt, Kirsten Alexandra; Veletzky, Luzia; Weinreich, Felix; Phillips, Richard Odame; Sarfo, Fred Stephen; Feldt, Torsten; Dompreh, Albert; Asibey, Shadrack Osei; Boateng, Richard; Frickmann, Hagen.
In: EUR J MICROBIOL IMMU, Vol. 13, No. 2, 13.10.2023, p. 57-62.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Very low prevalence of Mansonella perstans-specific cell-free DNA in serum samples of Ghanaian HIV patients
AU - Eberhardt, Kirsten Alexandra
AU - Veletzky, Luzia
AU - Weinreich, Felix
AU - Phillips, Richard Odame
AU - Sarfo, Fred Stephen
AU - Feldt, Torsten
AU - Dompreh, Albert
AU - Asibey, Shadrack Osei
AU - Boateng, Richard
AU - Frickmann, Hagen
PY - 2023/10/13
Y1 - 2023/10/13
N2 - BACKGROUND: Mansonellosis is a widely neglected helminth disease which is predominantly observed in tropical regions. This study was conducted to assess potential associations of the prevalence of circulating Mansonella perstans-specific cell-free DNA in human serum and HIV infection in Ghanaian individuals.METHODS: For this purpose, serum samples obtained from Ghanaian HIV-patients (n = 989) and non-HIV-infected Ghanaian control individuals (n = 91) were subjected to real-time PCR targeting the ITS-(internal transcribed spacer-)2 sequence of M. perstans and Mansonella sp. Deux.RESULTS: Mansonella-specific cell-free DNA was detected in serum samples of only 2 HIV-positive and 0 HIV-negative individuals, making any reliable conclusions on potential associations between HIV and mansonellosis in tropical Ghana unfeasible.CONCLUSIONS: Future epidemiological studies on hypothetical associations between mansonellosis and HIV infections should focus more specifically on high-endemicity settings for both Mansonella spp.-infections and HIV-infections, include higher case numbers and be based on real-time PCR from whole blood rather than from serum, in which only circulating parasite DNA but no more cell-bound parasite DNA can be detected. However, the study did not show associations of HIV infections in Ghanaian individuals with Mansonella worm loads high enough to detect cell-free Mansonella DNA in serum by PCR.
AB - BACKGROUND: Mansonellosis is a widely neglected helminth disease which is predominantly observed in tropical regions. This study was conducted to assess potential associations of the prevalence of circulating Mansonella perstans-specific cell-free DNA in human serum and HIV infection in Ghanaian individuals.METHODS: For this purpose, serum samples obtained from Ghanaian HIV-patients (n = 989) and non-HIV-infected Ghanaian control individuals (n = 91) were subjected to real-time PCR targeting the ITS-(internal transcribed spacer-)2 sequence of M. perstans and Mansonella sp. Deux.RESULTS: Mansonella-specific cell-free DNA was detected in serum samples of only 2 HIV-positive and 0 HIV-negative individuals, making any reliable conclusions on potential associations between HIV and mansonellosis in tropical Ghana unfeasible.CONCLUSIONS: Future epidemiological studies on hypothetical associations between mansonellosis and HIV infections should focus more specifically on high-endemicity settings for both Mansonella spp.-infections and HIV-infections, include higher case numbers and be based on real-time PCR from whole blood rather than from serum, in which only circulating parasite DNA but no more cell-bound parasite DNA can be detected. However, the study did not show associations of HIV infections in Ghanaian individuals with Mansonella worm loads high enough to detect cell-free Mansonella DNA in serum by PCR.
U2 - 10.1556/1886.2023.00028
DO - 10.1556/1886.2023.00028
M3 - SCORING: Journal article
C2 - 37751320
VL - 13
SP - 57
EP - 62
JO - EUR J MICROBIOL IMMU
JF - EUR J MICROBIOL IMMU
SN - 2062-8633
IS - 2
ER -