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UPF1 regulates myeloid cell functions and S100A9 expression by the hnRNP E2/miRNA-328 balance

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UPF1 regulates myeloid cell functions and S100A9 expression by the hnRNP E2/miRNA-328 balance. / Saul, Meike J; Stein, Stefan; Grez, Manuel; Jakobsson, Per-Johan; Steinhilber, Dieter; Suess, Beatrix.

In: SCI REP-UK, Vol. 6, 30.08.2016, p. 31995.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Saul, MJ, Stein, S, Grez, M, Jakobsson, P-J, Steinhilber, D & Suess, B 2016, 'UPF1 regulates myeloid cell functions and S100A9 expression by the hnRNP E2/miRNA-328 balance', SCI REP-UK, vol. 6, pp. 31995. https://doi.org/10.1038/srep31995

APA

Saul, M. J., Stein, S., Grez, M., Jakobsson, P-J., Steinhilber, D., & Suess, B. (2016). UPF1 regulates myeloid cell functions and S100A9 expression by the hnRNP E2/miRNA-328 balance. SCI REP-UK, 6, 31995. https://doi.org/10.1038/srep31995

Vancouver

Saul MJ, Stein S, Grez M, Jakobsson P-J, Steinhilber D, Suess B. UPF1 regulates myeloid cell functions and S100A9 expression by the hnRNP E2/miRNA-328 balance. SCI REP-UK. 2016 Aug 30;6:31995. https://doi.org/10.1038/srep31995

Bibtex

@article{341b880b9e0e4404a4c5d7128b4414e1,
title = "UPF1 regulates myeloid cell functions and S100A9 expression by the hnRNP E2/miRNA-328 balance",
abstract = "UPF1 is a key player in nonsense mediated mRNA decay (NMD) but also involved in posttranscriptional gene regulation. In this study we found that UPF1 regulates the expression of genes with functions in inflammation and myeloid cell differentiation via hnRNP E2. The majority of the UPF1-regulated genes identified in monocytic cells contain a binding site for hnRNP E2 within 5' UTR located introns with hnRNP E2 acting here as splicing regulator. We found that miRNA-328 which is significantly induced during monocytic cell differentiation acts independently from its gene silencing function as RNA decoy for hnRNP E2. One representative gene controlled by the hnRNP E2/miRNA-328 balance is S100A9 which plays an important role in cell differentiation and oxidative stress response of monocytes. Induction of miRNA-328 expression during cell differentiation antagonizes the blockade by hnRNP E2 which results in the upregulation of CD11b expression and ROS production in monocytic cells. Taken together, our data indicate that upregulation of miR-328 is responsible for the induction of hnRNP E2 target genes during myeloid cell differentiation.",
keywords = "CD11b Antigen/metabolism, Calgranulin B/metabolism, Cell Adhesion, Cell Differentiation, Cell Line, Cell Movement, Gene Expression Regulation, HeLa Cells, Humans, MicroRNAs/metabolism, Monocytes/cytology, Proteomics, RNA Helicases/metabolism, RNA Interference, RNA-Binding Proteins/genetics, Reactive Oxygen Species/metabolism, Trans-Activators/metabolism",
author = "Saul, {Meike J} and Stefan Stein and Manuel Grez and Per-Johan Jakobsson and Dieter Steinhilber and Beatrix Suess",
year = "2016",
month = aug,
day = "30",
doi = "10.1038/srep31995",
language = "English",
volume = "6",
pages = "31995",
journal = "SCI REP-UK",
issn = "2045-2322",
publisher = "NATURE PUBLISHING GROUP",

}

RIS

TY - JOUR

T1 - UPF1 regulates myeloid cell functions and S100A9 expression by the hnRNP E2/miRNA-328 balance

AU - Saul, Meike J

AU - Stein, Stefan

AU - Grez, Manuel

AU - Jakobsson, Per-Johan

AU - Steinhilber, Dieter

AU - Suess, Beatrix

PY - 2016/8/30

Y1 - 2016/8/30

N2 - UPF1 is a key player in nonsense mediated mRNA decay (NMD) but also involved in posttranscriptional gene regulation. In this study we found that UPF1 regulates the expression of genes with functions in inflammation and myeloid cell differentiation via hnRNP E2. The majority of the UPF1-regulated genes identified in monocytic cells contain a binding site for hnRNP E2 within 5' UTR located introns with hnRNP E2 acting here as splicing regulator. We found that miRNA-328 which is significantly induced during monocytic cell differentiation acts independently from its gene silencing function as RNA decoy for hnRNP E2. One representative gene controlled by the hnRNP E2/miRNA-328 balance is S100A9 which plays an important role in cell differentiation and oxidative stress response of monocytes. Induction of miRNA-328 expression during cell differentiation antagonizes the blockade by hnRNP E2 which results in the upregulation of CD11b expression and ROS production in monocytic cells. Taken together, our data indicate that upregulation of miR-328 is responsible for the induction of hnRNP E2 target genes during myeloid cell differentiation.

AB - UPF1 is a key player in nonsense mediated mRNA decay (NMD) but also involved in posttranscriptional gene regulation. In this study we found that UPF1 regulates the expression of genes with functions in inflammation and myeloid cell differentiation via hnRNP E2. The majority of the UPF1-regulated genes identified in monocytic cells contain a binding site for hnRNP E2 within 5' UTR located introns with hnRNP E2 acting here as splicing regulator. We found that miRNA-328 which is significantly induced during monocytic cell differentiation acts independently from its gene silencing function as RNA decoy for hnRNP E2. One representative gene controlled by the hnRNP E2/miRNA-328 balance is S100A9 which plays an important role in cell differentiation and oxidative stress response of monocytes. Induction of miRNA-328 expression during cell differentiation antagonizes the blockade by hnRNP E2 which results in the upregulation of CD11b expression and ROS production in monocytic cells. Taken together, our data indicate that upregulation of miR-328 is responsible for the induction of hnRNP E2 target genes during myeloid cell differentiation.

KW - CD11b Antigen/metabolism

KW - Calgranulin B/metabolism

KW - Cell Adhesion

KW - Cell Differentiation

KW - Cell Line

KW - Cell Movement

KW - Gene Expression Regulation

KW - HeLa Cells

KW - Humans

KW - MicroRNAs/metabolism

KW - Monocytes/cytology

KW - Proteomics

KW - RNA Helicases/metabolism

KW - RNA Interference

KW - RNA-Binding Proteins/genetics

KW - Reactive Oxygen Species/metabolism

KW - Trans-Activators/metabolism

U2 - 10.1038/srep31995

DO - 10.1038/srep31995

M3 - SCORING: Journal article

C2 - 27573788

VL - 6

SP - 31995

JO - SCI REP-UK

JF - SCI REP-UK

SN - 2045-2322

ER -