Ultrasensitive detection of prostate specific antigen in the followup of 422 patients after radical prostatectomy.
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Ultrasensitive detection of prostate specific antigen in the followup of 422 patients after radical prostatectomy. / Haese, Alexander; Huland, E; Graefen, M; Hammerer, P; Noldus, J; Huland, H.
In: J UROLOGY, Vol. 161, No. 4, 4, 1999, p. 1206-1211.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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T1 - Ultrasensitive detection of prostate specific antigen in the followup of 422 patients after radical prostatectomy.
AU - Haese, Alexander
AU - Huland, E
AU - Graefen, M
AU - Hammerer, P
AU - Noldus, J
AU - Huland, H
PY - 1999
Y1 - 1999
N2 - PURPOSE: We validated our ultrasensitive prostate specific antigen (PSA) assay based on lyophilization and 4-fold concentration of patient sera with the clinical long-term followup and according to histopathological characteristics of 422 patients treated with radical retropubic prostatectomy for prostate cancer. MATERIALS AND METHODS: Each serum sample was divided into 2 aliquots for standard and 4-fold concentrated (ultrasensitive) detection. Samples were analyzed by the same unmodified DPC-Immulite PSA assay. Biochemical relapse was defined as an increase of at least 0.10 ng./ml. in native serum (equivalent to 0.025 ng./ml. in concentrated serum). Mean followup was 449 days (range 29 to 2,057). Kaplan-Meier analysis of standard and ultrasensitive detection results was done, and findings were correlated with pathological stage, Gleason grade, total cancer volume, Gleason grade 4 cancer volume and margin status. Significance of earlier detection in ultrasensitive versus standard detection was calculated with the log rank (Mantel-Cox) test with p
AB - PURPOSE: We validated our ultrasensitive prostate specific antigen (PSA) assay based on lyophilization and 4-fold concentration of patient sera with the clinical long-term followup and according to histopathological characteristics of 422 patients treated with radical retropubic prostatectomy for prostate cancer. MATERIALS AND METHODS: Each serum sample was divided into 2 aliquots for standard and 4-fold concentrated (ultrasensitive) detection. Samples were analyzed by the same unmodified DPC-Immulite PSA assay. Biochemical relapse was defined as an increase of at least 0.10 ng./ml. in native serum (equivalent to 0.025 ng./ml. in concentrated serum). Mean followup was 449 days (range 29 to 2,057). Kaplan-Meier analysis of standard and ultrasensitive detection results was done, and findings were correlated with pathological stage, Gleason grade, total cancer volume, Gleason grade 4 cancer volume and margin status. Significance of earlier detection in ultrasensitive versus standard detection was calculated with the log rank (Mantel-Cox) test with p
M3 - SCORING: Zeitschriftenaufsatz
VL - 161
SP - 1206
EP - 1211
JO - J UROLOGY
JF - J UROLOGY
SN - 0022-5347
IS - 4
M1 - 4
ER -