Ubiquitin-negative mini-pick-like bodies in the dentate gyrus in p301l tauopathy
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Ubiquitin-negative mini-pick-like bodies in the dentate gyrus in p301l tauopathy. / Ferrer, Isidro; Hernández, Isabel; Puig, Berta; Rey, María Jesús; Ezquerra, Mario; Tolosa, Eduardo; Boada, Merce; Puig Martorell, Berta.
In: J ALZHEIMERS DIS, Vol. 5, No. 6, 01.12.2003, p. 445-54.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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T1 - Ubiquitin-negative mini-pick-like bodies in the dentate gyrus in p301l tauopathy
AU - Ferrer, Isidro
AU - Hernández, Isabel
AU - Puig, Berta
AU - Rey, María Jesús
AU - Ezquerra, Mario
AU - Tolosa, Eduardo
AU - Boada, Merce
AU - Puig Martorell, Berta
PY - 2003/12/1
Y1 - 2003/12/1
N2 - Neuropathological and biochemical findings are reported in a patient who had suffered from frontotemporal dementia associated with a P310L mutation in the tau gene and included in the H1 haplotype. Tau accumulation, as revealed with phospho-specific anti-tau antibodies Thr181, Ser199, Ser202, Ser214, Ser262, Ser396, Ser422 and AT8 (Ser202 and Thr205), was found in neurons with pre-tangles, and astrocytes and oligodendrocytes through the brain. The most characteristic feature was tau immunoreactivity decorating the perinuclear region and small cytoplasmic aggregates designed as mini-Pick-like bodies, mainly in the dentate gyrus. Inclusions were not stained with anti-ubiquitin antibodies and did not recruit tubulins. Tau accumulation in individual cells was associated with increased expression of kinases linked with tau phosphorylation, mainly active (phosphorylated) stress kinases SAPK/JNK and p38 (SAPK/JNK-P and p38-P). Phosphorylated GSK-3 beta at Ser9 (GSK-3 beta-P), that inactivates the kinase, was particularly abundant in mini-Pick-like bodies, thus suggesting alternative roles of GSK-3 probably involved in cell survival. Western blots of sarkosyl-insoluble fractions revealed a double band pattern of phospho-tau of 68/66 kDa and 64 kDa in the hippocampus and white matter in the P310L mutation. Sarkosyl-insoluble fractions of the hippocampus were enriched in p38-P and GSK-3 beta-P in Alzheimer's disease (AD) cases, processed in parallel for comparative purposes, but not in the P310L mutation. In addition, no bands of high molecular weight were found in P310L in contrast with AD in these fractions. These findings indicate that the major sites of tau phosphorylation, and the expression of kinases involved in tau phosphorylation are active in P310L mutation as in AD and other tauopathies. Yet the P310L mutation has particular phospho-tau inclusions that are not tag with ubiquitin and appear to be rather soluble when compared with AD.
AB - Neuropathological and biochemical findings are reported in a patient who had suffered from frontotemporal dementia associated with a P310L mutation in the tau gene and included in the H1 haplotype. Tau accumulation, as revealed with phospho-specific anti-tau antibodies Thr181, Ser199, Ser202, Ser214, Ser262, Ser396, Ser422 and AT8 (Ser202 and Thr205), was found in neurons with pre-tangles, and astrocytes and oligodendrocytes through the brain. The most characteristic feature was tau immunoreactivity decorating the perinuclear region and small cytoplasmic aggregates designed as mini-Pick-like bodies, mainly in the dentate gyrus. Inclusions were not stained with anti-ubiquitin antibodies and did not recruit tubulins. Tau accumulation in individual cells was associated with increased expression of kinases linked with tau phosphorylation, mainly active (phosphorylated) stress kinases SAPK/JNK and p38 (SAPK/JNK-P and p38-P). Phosphorylated GSK-3 beta at Ser9 (GSK-3 beta-P), that inactivates the kinase, was particularly abundant in mini-Pick-like bodies, thus suggesting alternative roles of GSK-3 probably involved in cell survival. Western blots of sarkosyl-insoluble fractions revealed a double band pattern of phospho-tau of 68/66 kDa and 64 kDa in the hippocampus and white matter in the P310L mutation. Sarkosyl-insoluble fractions of the hippocampus were enriched in p38-P and GSK-3 beta-P in Alzheimer's disease (AD) cases, processed in parallel for comparative purposes, but not in the P310L mutation. In addition, no bands of high molecular weight were found in P310L in contrast with AD in these fractions. These findings indicate that the major sites of tau phosphorylation, and the expression of kinases involved in tau phosphorylation are active in P310L mutation as in AD and other tauopathies. Yet the P310L mutation has particular phospho-tau inclusions that are not tag with ubiquitin and appear to be rather soluble when compared with AD.
KW - Antibodies, Anti-Idiotypic
KW - Blotting, Western
KW - Dentate Gyrus
KW - Fatal Outcome
KW - Frontal Lobe
KW - Gene Expression
KW - Hippocampus
KW - Humans
KW - Immunohistochemistry
KW - Male
KW - Middle Aged
KW - Neurons
KW - Phosphorylase Kinase
KW - Phosphorylation
KW - Pick Disease of the Brain
KW - Point Mutation
KW - Temporal Lobe
KW - Ubiquitin
KW - tau Proteins
M3 - SCORING: Journal article
C2 - 14757934
VL - 5
SP - 445
EP - 454
JO - J ALZHEIMERS DIS
JF - J ALZHEIMERS DIS
SN - 1387-2877
IS - 6
ER -