TrkC Is Essential for Nephron Function and Trans-Activates Igf1R Signaling
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TrkC Is Essential for Nephron Function and Trans-Activates Igf1R Signaling. / Lepa, Carolin; Hoppe, Sascha; Stöber, Antje; Skryabin, Boris V; Sievers, Laura Katharina; Heitplatz, Barbara; Ciarimboli, Giuliano; Neugebauer, Ute; Lindenmeyer, Maja T; Cohen, Clemens D; Drexler, Hannes C A; Boor, Peter; Weide, Thomas; Pavenstädt, Hermann; George, Britta.
In: J AM SOC NEPHROL, Vol. 32, No. 2, 02.2021, p. 357-374.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - TrkC Is Essential for Nephron Function and Trans-Activates Igf1R Signaling
AU - Lepa, Carolin
AU - Hoppe, Sascha
AU - Stöber, Antje
AU - Skryabin, Boris V
AU - Sievers, Laura Katharina
AU - Heitplatz, Barbara
AU - Ciarimboli, Giuliano
AU - Neugebauer, Ute
AU - Lindenmeyer, Maja T
AU - Cohen, Clemens D
AU - Drexler, Hannes C A
AU - Boor, Peter
AU - Weide, Thomas
AU - Pavenstädt, Hermann
AU - George, Britta
N1 - Copyright © 2021 by the American Society of Nephrology.
PY - 2021/2
Y1 - 2021/2
N2 - BACKGROUND: Injury to kidney podocytes often results in chronic glomerular disease and consecutive nephron malfunction. For most glomerular diseases, targeted therapies are lacking. Thus, it is important to identify novel signaling pathways contributing to glomerular disease. Neurotrophic tyrosine kinase receptor 3 (TrkC) is expressed in podocytes and the protein transmits signals to the podocyte actin cytoskeleton.METHODS: Nephron-specific TrkC knockout (TrkC-KO) and nephron-specific TrkC-overexpressing (TrkC-OE) mice were generated to dissect the role of TrkC in nephron development and maintenance.RESULTS: Both TrkC-KO and TrkC-OE mice exhibited enlarged glomeruli, mesangial proliferation, basement membrane thickening, albuminuria, podocyte loss, and aspects of FSGS during aging. Igf1 receptor (Igf1R)-associated gene expression was dysregulated in TrkC-KO mouse glomeruli. Phosphoproteins associated with insulin, erb-b2 receptor tyrosine kinase (Erbb), and Toll-like receptor signaling were enriched in lysates of podocytes treated with the TrkC ligand neurotrophin-3 (Nt-3). Activation of TrkC by Nt-3 resulted in phosphorylation of the Igf1R on activating tyrosine residues in podocytes. Igf1R phosphorylation was increased in TrkC-OE mouse kidneys while it was decreased in TrkC-KO kidneys. Furthermore, TrkC expression was elevated in glomerular tissue of patients with diabetic kidney disease compared with control glomerular tissue.CONCLUSIONS: Our results show that TrkC is essential for maintaining glomerular integrity. Furthermore, TrkC modulates Igf-related signaling in podocytes.
AB - BACKGROUND: Injury to kidney podocytes often results in chronic glomerular disease and consecutive nephron malfunction. For most glomerular diseases, targeted therapies are lacking. Thus, it is important to identify novel signaling pathways contributing to glomerular disease. Neurotrophic tyrosine kinase receptor 3 (TrkC) is expressed in podocytes and the protein transmits signals to the podocyte actin cytoskeleton.METHODS: Nephron-specific TrkC knockout (TrkC-KO) and nephron-specific TrkC-overexpressing (TrkC-OE) mice were generated to dissect the role of TrkC in nephron development and maintenance.RESULTS: Both TrkC-KO and TrkC-OE mice exhibited enlarged glomeruli, mesangial proliferation, basement membrane thickening, albuminuria, podocyte loss, and aspects of FSGS during aging. Igf1 receptor (Igf1R)-associated gene expression was dysregulated in TrkC-KO mouse glomeruli. Phosphoproteins associated with insulin, erb-b2 receptor tyrosine kinase (Erbb), and Toll-like receptor signaling were enriched in lysates of podocytes treated with the TrkC ligand neurotrophin-3 (Nt-3). Activation of TrkC by Nt-3 resulted in phosphorylation of the Igf1R on activating tyrosine residues in podocytes. Igf1R phosphorylation was increased in TrkC-OE mouse kidneys while it was decreased in TrkC-KO kidneys. Furthermore, TrkC expression was elevated in glomerular tissue of patients with diabetic kidney disease compared with control glomerular tissue.CONCLUSIONS: Our results show that TrkC is essential for maintaining glomerular integrity. Furthermore, TrkC modulates Igf-related signaling in podocytes.
U2 - 10.1681/ASN.2020040424
DO - 10.1681/ASN.2020040424
M3 - SCORING: Journal article
C2 - 33380522
VL - 32
SP - 357
EP - 374
JO - J AM SOC NEPHROL
JF - J AM SOC NEPHROL
SN - 1046-6673
IS - 2
ER -