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Translation of atherosclerotic plaque phase-contrast CT imaging from synchrotron radiation to a conventional lab-based X-ray source

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Translation of atherosclerotic plaque phase-contrast CT imaging from synchrotron radiation to a conventional lab-based X-ray source. / Saam, Tobias; Herzen, Julia; Hetterich, Holger; Fill, Sandra; Willner, Marian; Stockmar, Marco; Achterhold, Klaus; Zanette, Irene; Weitkamp, Timm; Schüller, Ulrich; Auweter, Sigrid; Adam-Neumair, Silvia; Nikolaou, Konstantin; Reiser, Maximilian F; Pfeiffer, Franz; Bamberg, Fabian.

In: PLOS ONE, Vol. 8, No. 9, 2013, p. e73513.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Saam, T, Herzen, J, Hetterich, H, Fill, S, Willner, M, Stockmar, M, Achterhold, K, Zanette, I, Weitkamp, T, Schüller, U, Auweter, S, Adam-Neumair, S, Nikolaou, K, Reiser, MF, Pfeiffer, F & Bamberg, F 2013, 'Translation of atherosclerotic plaque phase-contrast CT imaging from synchrotron radiation to a conventional lab-based X-ray source', PLOS ONE, vol. 8, no. 9, pp. e73513. https://doi.org/10.1371/journal.pone.0073513

APA

Saam, T., Herzen, J., Hetterich, H., Fill, S., Willner, M., Stockmar, M., Achterhold, K., Zanette, I., Weitkamp, T., Schüller, U., Auweter, S., Adam-Neumair, S., Nikolaou, K., Reiser, M. F., Pfeiffer, F., & Bamberg, F. (2013). Translation of atherosclerotic plaque phase-contrast CT imaging from synchrotron radiation to a conventional lab-based X-ray source. PLOS ONE, 8(9), e73513. https://doi.org/10.1371/journal.pone.0073513

Vancouver

Saam T, Herzen J, Hetterich H, Fill S, Willner M, Stockmar M et al. Translation of atherosclerotic plaque phase-contrast CT imaging from synchrotron radiation to a conventional lab-based X-ray source. PLOS ONE. 2013;8(9):e73513. https://doi.org/10.1371/journal.pone.0073513

Bibtex

@article{60f8f3bb7eca44b3b417e60f53390821,
title = "Translation of atherosclerotic plaque phase-contrast CT imaging from synchrotron radiation to a conventional lab-based X-ray source",
abstract = "OBJECTIVES: Phase-contrast imaging is a novel X-ray based technique that provides enhanced soft tissue contrast. The aim of this study was to evaluate the feasibility of visualizing human carotid arteries by grating-based phase-contrast tomography (PC-CT) at two different experimental set-ups: (i) applying synchrotron radiation and (ii) using a conventional X-ray tube.MATERIALS AND METHODS: Five ex-vivo carotid artery specimens were examined with PC-CT either at the European Synchrotron Radiation Facility using a monochromatic X-ray beam (2 specimens; 23 keV; pixel size 5.4 µm), or at a laboratory set-up on a conventional X-ray tube (3 specimens; 35-40 kVp; 70 mA; pixel size 100 µm). Tomographic images were reconstructed and compared to histopathology. Two independent readers determined vessel dimensions and one reader determined signal-to-noise ratios (SNR) between PC-CT and absorption images.RESULTS: In total, 51 sections were included in the analysis. Images from both set-ups provided sufficient contrast to differentiate individual vessel layers. All PCI-based measurements strongly predicted but significantly overestimated lumen, intima and vessel wall area for both the synchrotron and the laboratory-based measurements as compared with histology (all p<0.001 with slope >0.53 per mm(2), 95%-CI: 0.35 to 0.70). Although synchrotron-based images were characterized by higher SNRs than laboratory-based images; both PC-CT set-ups had superior SNRs compared to corresponding conventional absorption-based images (p<0.001). Inter-reader reproducibility was excellent (ICCs >0.98 and >0.84 for synchrotron and for laboratory-based measurements; respectively).CONCLUSION: Experimental PC-CT of carotid specimens is feasible with both synchrotron and conventional X-ray sources, producing high-resolution images suitable for vessel characterization and atherosclerosis research.",
keywords = "Carotid Arteries, Equipment Design, Humans, Plaque, Atherosclerotic, Reproducibility of Results, Synchrotrons, Tomography, X-Ray Computed, Evaluation Studies, Journal Article, Research Support, Non-U.S. Gov't",
author = "Tobias Saam and Julia Herzen and Holger Hetterich and Sandra Fill and Marian Willner and Marco Stockmar and Klaus Achterhold and Irene Zanette and Timm Weitkamp and Ulrich Sch{\"u}ller and Sigrid Auweter and Silvia Adam-Neumair and Konstantin Nikolaou and Reiser, {Maximilian F} and Franz Pfeiffer and Fabian Bamberg",
year = "2013",
doi = "10.1371/journal.pone.0073513",
language = "English",
volume = "8",
pages = "e73513",
journal = "PLOS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "9",

}

RIS

TY - JOUR

T1 - Translation of atherosclerotic plaque phase-contrast CT imaging from synchrotron radiation to a conventional lab-based X-ray source

AU - Saam, Tobias

AU - Herzen, Julia

AU - Hetterich, Holger

AU - Fill, Sandra

AU - Willner, Marian

AU - Stockmar, Marco

AU - Achterhold, Klaus

AU - Zanette, Irene

AU - Weitkamp, Timm

AU - Schüller, Ulrich

AU - Auweter, Sigrid

AU - Adam-Neumair, Silvia

AU - Nikolaou, Konstantin

AU - Reiser, Maximilian F

AU - Pfeiffer, Franz

AU - Bamberg, Fabian

PY - 2013

Y1 - 2013

N2 - OBJECTIVES: Phase-contrast imaging is a novel X-ray based technique that provides enhanced soft tissue contrast. The aim of this study was to evaluate the feasibility of visualizing human carotid arteries by grating-based phase-contrast tomography (PC-CT) at two different experimental set-ups: (i) applying synchrotron radiation and (ii) using a conventional X-ray tube.MATERIALS AND METHODS: Five ex-vivo carotid artery specimens were examined with PC-CT either at the European Synchrotron Radiation Facility using a monochromatic X-ray beam (2 specimens; 23 keV; pixel size 5.4 µm), or at a laboratory set-up on a conventional X-ray tube (3 specimens; 35-40 kVp; 70 mA; pixel size 100 µm). Tomographic images were reconstructed and compared to histopathology. Two independent readers determined vessel dimensions and one reader determined signal-to-noise ratios (SNR) between PC-CT and absorption images.RESULTS: In total, 51 sections were included in the analysis. Images from both set-ups provided sufficient contrast to differentiate individual vessel layers. All PCI-based measurements strongly predicted but significantly overestimated lumen, intima and vessel wall area for both the synchrotron and the laboratory-based measurements as compared with histology (all p<0.001 with slope >0.53 per mm(2), 95%-CI: 0.35 to 0.70). Although synchrotron-based images were characterized by higher SNRs than laboratory-based images; both PC-CT set-ups had superior SNRs compared to corresponding conventional absorption-based images (p<0.001). Inter-reader reproducibility was excellent (ICCs >0.98 and >0.84 for synchrotron and for laboratory-based measurements; respectively).CONCLUSION: Experimental PC-CT of carotid specimens is feasible with both synchrotron and conventional X-ray sources, producing high-resolution images suitable for vessel characterization and atherosclerosis research.

AB - OBJECTIVES: Phase-contrast imaging is a novel X-ray based technique that provides enhanced soft tissue contrast. The aim of this study was to evaluate the feasibility of visualizing human carotid arteries by grating-based phase-contrast tomography (PC-CT) at two different experimental set-ups: (i) applying synchrotron radiation and (ii) using a conventional X-ray tube.MATERIALS AND METHODS: Five ex-vivo carotid artery specimens were examined with PC-CT either at the European Synchrotron Radiation Facility using a monochromatic X-ray beam (2 specimens; 23 keV; pixel size 5.4 µm), or at a laboratory set-up on a conventional X-ray tube (3 specimens; 35-40 kVp; 70 mA; pixel size 100 µm). Tomographic images were reconstructed and compared to histopathology. Two independent readers determined vessel dimensions and one reader determined signal-to-noise ratios (SNR) between PC-CT and absorption images.RESULTS: In total, 51 sections were included in the analysis. Images from both set-ups provided sufficient contrast to differentiate individual vessel layers. All PCI-based measurements strongly predicted but significantly overestimated lumen, intima and vessel wall area for both the synchrotron and the laboratory-based measurements as compared with histology (all p<0.001 with slope >0.53 per mm(2), 95%-CI: 0.35 to 0.70). Although synchrotron-based images were characterized by higher SNRs than laboratory-based images; both PC-CT set-ups had superior SNRs compared to corresponding conventional absorption-based images (p<0.001). Inter-reader reproducibility was excellent (ICCs >0.98 and >0.84 for synchrotron and for laboratory-based measurements; respectively).CONCLUSION: Experimental PC-CT of carotid specimens is feasible with both synchrotron and conventional X-ray sources, producing high-resolution images suitable for vessel characterization and atherosclerosis research.

KW - Carotid Arteries

KW - Equipment Design

KW - Humans

KW - Plaque, Atherosclerotic

KW - Reproducibility of Results

KW - Synchrotrons

KW - Tomography, X-Ray Computed

KW - Evaluation Studies

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1371/journal.pone.0073513

DO - 10.1371/journal.pone.0073513

M3 - SCORING: Journal article

C2 - 24039969

VL - 8

SP - e73513

JO - PLOS ONE

JF - PLOS ONE

SN - 1932-6203

IS - 9

ER -