Transient expression of NMDA receptor subunit NR2B in the developing rat heart.
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Transient expression of NMDA receptor subunit NR2B in the developing rat heart. / Seeber, S; Becker, K; Rau, Thomas; Eschenhagen, T; Becker, C M; Herkert, M.
In: J NEUROCHEM, Vol. 75, No. 6, 6, 2000, p. 2472-2477.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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T1 - Transient expression of NMDA receptor subunit NR2B in the developing rat heart.
AU - Seeber, S
AU - Becker, K
AU - Rau, Thomas
AU - Eschenhagen, T
AU - Becker, C M
AU - Herkert, M
PY - 2000
Y1 - 2000
N2 - NMDA receptors represent a subtype of the ionotropic glutamate receptor family, comprising three classes of subunits (NR1, NR2A-D, NR3), which exhibit distinct patterns of regional and developmental expression in the CNS. Recently, some NMDA receptor subunits have also been described in adult extraneuronal tissues and keratinocytes. However, their developmental expression patterns are currently unknown. With use of RT-PCR and western blot analysis, the expression of NMDA receptor subunit NR2B was investigated in the developing rat heart. NR2B mRNA and protein were detected in heart tissue of rats from embryonic day 14 until postnatal day 21 but disappeared 10 weeks after birth. In contrast, no NMDA receptor subunit NR1, alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid receptor subunit GluR2, or anchoring postsynaptic density protein-95 could be detected in rat heart at any developmental stage. Confocal microscopy of cultured cardiac myocytes (CMs) from neonatal rats revealed distinct NR2B staining mainly of intracellular structures. However, no functional NMDA receptor could be detected on CMs by whole-cell recordings. In conclusion, high concentrations of NR2B protein can be detected in early rat heart development, but its function still remains elusive.
AB - NMDA receptors represent a subtype of the ionotropic glutamate receptor family, comprising three classes of subunits (NR1, NR2A-D, NR3), which exhibit distinct patterns of regional and developmental expression in the CNS. Recently, some NMDA receptor subunits have also been described in adult extraneuronal tissues and keratinocytes. However, their developmental expression patterns are currently unknown. With use of RT-PCR and western blot analysis, the expression of NMDA receptor subunit NR2B was investigated in the developing rat heart. NR2B mRNA and protein were detected in heart tissue of rats from embryonic day 14 until postnatal day 21 but disappeared 10 weeks after birth. In contrast, no NMDA receptor subunit NR1, alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid receptor subunit GluR2, or anchoring postsynaptic density protein-95 could be detected in rat heart at any developmental stage. Confocal microscopy of cultured cardiac myocytes (CMs) from neonatal rats revealed distinct NR2B staining mainly of intracellular structures. However, no functional NMDA receptor could be detected on CMs by whole-cell recordings. In conclusion, high concentrations of NR2B protein can be detected in early rat heart development, but its function still remains elusive.
M3 - SCORING: Zeitschriftenaufsatz
VL - 75
SP - 2472
EP - 2477
JO - J NEUROCHEM
JF - J NEUROCHEM
SN - 0022-3042
IS - 6
M1 - 6
ER -
