Transient complex interactions of mammalian peroxisomes without exchange of matrix or membrane marker proteins.
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Transient complex interactions of mammalian peroxisomes without exchange of matrix or membrane marker proteins. / Bonekamp, Nina A; Sampaio, Paula; Abreu, de; Lüers, Georg Hermann; Schrader, Michael.
In: TRAFFIC, Vol. 13, No. 7, 7, 2012, p. 960-978.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Transient complex interactions of mammalian peroxisomes without exchange of matrix or membrane marker proteins.
AU - Bonekamp, Nina A
AU - Sampaio, Paula
AU - Abreu, de
AU - Lüers, Georg Hermann
AU - Schrader, Michael
PY - 2012
Y1 - 2012
N2 - Peroxisomes and mitochondria show a much closer interrelationship than previously anticipated. They co-operate in the metabolism of fatty acids and reactive oxygen species, but also share components of their fission machinery. If peroxisomes - like mitochondria - also fuse in mammalian cells is a matter of debate and was not yet systematically investigated. To examine potential peroxisomal fusion and interactions in mammalian cells, we established an in vivo fusion assay based on hybridoma formation by cell fusion. Fluorescence microscopy in time course experiments revealed a merge of different peroxisomal markers in fused cells. However, live cell imaging revealed that peroxisomes were engaged in transient and long-term contacts, without exchanging matrix or membrane markers. Computational analysis showed that transient peroxisomal interactions are complex and can potentially contribute to the homogenization of the peroxisomal compartment. However, peroxisomal interactions do not increase after fatty acid or H(2) O(2) treatment. Additionally, we provide the first evidence that mitochondrial fusion proteins do not localize to peroxisomes. We conclude that mammalian peroxisomes do not fuse with each other in a mechanism similar to mitochondrial fusion. However, they show an extensive degree of interaction, the implication of which is discussed.
AB - Peroxisomes and mitochondria show a much closer interrelationship than previously anticipated. They co-operate in the metabolism of fatty acids and reactive oxygen species, but also share components of their fission machinery. If peroxisomes - like mitochondria - also fuse in mammalian cells is a matter of debate and was not yet systematically investigated. To examine potential peroxisomal fusion and interactions in mammalian cells, we established an in vivo fusion assay based on hybridoma formation by cell fusion. Fluorescence microscopy in time course experiments revealed a merge of different peroxisomal markers in fused cells. However, live cell imaging revealed that peroxisomes were engaged in transient and long-term contacts, without exchanging matrix or membrane markers. Computational analysis showed that transient peroxisomal interactions are complex and can potentially contribute to the homogenization of the peroxisomal compartment. However, peroxisomal interactions do not increase after fatty acid or H(2) O(2) treatment. Additionally, we provide the first evidence that mitochondrial fusion proteins do not localize to peroxisomes. We conclude that mammalian peroxisomes do not fuse with each other in a mechanism similar to mitochondrial fusion. However, they show an extensive degree of interaction, the implication of which is discussed.
KW - Animals
KW - CHO Cells
KW - Cricetinae
KW - Cricetulus
KW - COS Cells
KW - Cercopithecus aethiops
KW - Microscopy, Fluorescence
KW - Biological Markers/analysis
KW - Membrane Proteins/analysis
KW - Cell Fusion/methods
KW - Hybridomas
KW - Membrane Fusion
KW - Mitochondria/physiology
KW - Peroxisomes/physiology
KW - Animals
KW - CHO Cells
KW - Cricetinae
KW - Cricetulus
KW - COS Cells
KW - Cercopithecus aethiops
KW - Microscopy, Fluorescence
KW - Biological Markers/analysis
KW - Membrane Proteins/analysis
KW - Cell Fusion/methods
KW - Hybridomas
KW - Membrane Fusion
KW - Mitochondria/physiology
KW - Peroxisomes/physiology
M3 - SCORING: Journal article
VL - 13
SP - 960
EP - 978
JO - TRAFFIC
JF - TRAFFIC
SN - 1398-9219
IS - 7
M1 - 7
ER -
