Transcription alterations of members of the ubiquitin-proteasome network in prostate carcinoma.

Olaf Hellwinkel; LE Asong; Jan-Peer J. Rogmann; H Sültmann; Christina Wagner; Thorsten Schlomm; Christian Eichelberg

Transcription alterations of members of the ubiquitin-proteasome network in prostate carcinoma.

Standard

Transcription alterations of members of the ubiquitin-proteasome network in prostate carcinoma. / Hellwinkel, Olaf; Asong, LE; Rogmann, Jan-Peer J.; Sültmann, H; Wagner, Christina; Schlomm, Thorsten; Eichelberg, Christian.

In: PROSTATE CANCER P D, Vol. 14, No. 1, 1, 2011, p. 38-45.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Hellwinkel, O, Asong, LE, Rogmann, J-PJ, Sültmann, H, Wagner, C, Schlomm, T & Eichelberg, C 2011, 'Transcription alterations of members of the ubiquitin-proteasome network in prostate carcinoma.', PROSTATE CANCER P D, vol. 14, no. 1, 1, pp. 38-45. <http://www.ncbi.nlm.nih.gov/pubmed/21102547?dopt=Citation>

APA

Hellwinkel, O., Asong, LE., Rogmann, J-P. J., Sültmann, H., Wagner, C., Schlomm, T., & Eichelberg, C. (2011). Transcription alterations of members of the ubiquitin-proteasome network in prostate carcinoma. PROSTATE CANCER P D, 14(1), 38-45. [1]. http://www.ncbi.nlm.nih.gov/pubmed/21102547?dopt=Citation

Vancouver

Hellwinkel O, Asong LE, Rogmann J-PJ, Sültmann H, Wagner C, Schlomm T et al. Transcription alterations of members of the ubiquitin-proteasome network in prostate carcinoma. PROSTATE CANCER P D. 2011;14(1):38-45. 1.

Bibtex

@article{b4b00729b1c94cbcaf99542a79210038,

title = "Transcription alterations of members of the ubiquitin-proteasome network in prostate carcinoma.",

abstract = "The purpose of this work was to investigate the role of the ubiquitin-proteasome network (UPN) in prostate cancer (PCA) and to elicit potential markers for this disease. The UPN represents a key factor in the maintenance of cellular homoeostasis as a result of its fundamental function in the regulation of intracellular protein degradation. Members of this network have a role in the biology of haematological and solid tumours. Tumour cells and normal epithelial cells from 22 prostatectomy specimens were isolated by laser microdissection. Prostate biopsy samples from healthy individuals served for technical calibration and as controls. Transcript levels of eight selected genes with E3 ubiquitin ligase activity (labelling target proteins for proteasome degradation) and two genes belonging to the proteasome-multienzyme complex itself were analysed by quantitative real-time RT-PCR. The proteasome genes PSMC4 and PSMB5 and the E3 ubiquitin ligase NEDD4L were significantly and coherently upregulated in PCA cells compared with the corresponding adjacent normal prostate tissue. Transcription of the E3 ubiquitin ligase SMURF2 was significantly higher in organ-confined tumours (pT2) compared with non-organ-confined cancers (pT3). The results indicate a role for PSMC4 and PSMB5 and the E3 ubiquitin ligase NEDD4L in prostate tumourigenesis, whereas SMURF2 downregulation could be associated with clinical progression. NEDD4L and SMURF2 both target transforming growth factor (TGF)-? for degradation. This reflects the pleiotropic role of the TGF-? signalling pathway acting as a tumour suppressor in normal and pre-cancerous cells, but having oncogenic properties in progressing cancer. Further studies have to elucidate whether these alterations could represent clinically relevant PCA-diagnostic and progression markers.",

author = "Olaf Hellwinkel and LE Asong and Rogmann, {Jan-Peer J.} and H S{\"u}ltmann and Christina Wagner and Thorsten Schlomm and Christian Eichelberg",

year = "2011",

language = "English",

volume = "14",

pages = "38--45",

journal = "PROSTATE CANCER P D",

issn = "1365-7852",

publisher = "NATURE PUBLISHING GROUP",

number = "1",

}

RIS

TY - JOUR

T1 - Transcription alterations of members of the ubiquitin-proteasome network in prostate carcinoma.

AU - Hellwinkel, Olaf

AU - Asong, LE

AU - Rogmann, Jan-Peer J.

AU - Sültmann, H

AU - Wagner, Christina

AU - Schlomm, Thorsten

AU - Eichelberg, Christian

PY - 2011

Y1 - 2011

N2 - The purpose of this work was to investigate the role of the ubiquitin-proteasome network (UPN) in prostate cancer (PCA) and to elicit potential markers for this disease. The UPN represents a key factor in the maintenance of cellular homoeostasis as a result of its fundamental function in the regulation of intracellular protein degradation. Members of this network have a role in the biology of haematological and solid tumours. Tumour cells and normal epithelial cells from 22 prostatectomy specimens were isolated by laser microdissection. Prostate biopsy samples from healthy individuals served for technical calibration and as controls. Transcript levels of eight selected genes with E3 ubiquitin ligase activity (labelling target proteins for proteasome degradation) and two genes belonging to the proteasome-multienzyme complex itself were analysed by quantitative real-time RT-PCR. The proteasome genes PSMC4 and PSMB5 and the E3 ubiquitin ligase NEDD4L were significantly and coherently upregulated in PCA cells compared with the corresponding adjacent normal prostate tissue. Transcription of the E3 ubiquitin ligase SMURF2 was significantly higher in organ-confined tumours (pT2) compared with non-organ-confined cancers (pT3). The results indicate a role for PSMC4 and PSMB5 and the E3 ubiquitin ligase NEDD4L in prostate tumourigenesis, whereas SMURF2 downregulation could be associated with clinical progression. NEDD4L and SMURF2 both target transforming growth factor (TGF)-? for degradation. This reflects the pleiotropic role of the TGF-? signalling pathway acting as a tumour suppressor in normal and pre-cancerous cells, but having oncogenic properties in progressing cancer. Further studies have to elucidate whether these alterations could represent clinically relevant PCA-diagnostic and progression markers.

AB - The purpose of this work was to investigate the role of the ubiquitin-proteasome network (UPN) in prostate cancer (PCA) and to elicit potential markers for this disease. The UPN represents a key factor in the maintenance of cellular homoeostasis as a result of its fundamental function in the regulation of intracellular protein degradation. Members of this network have a role in the biology of haematological and solid tumours. Tumour cells and normal epithelial cells from 22 prostatectomy specimens were isolated by laser microdissection. Prostate biopsy samples from healthy individuals served for technical calibration and as controls. Transcript levels of eight selected genes with E3 ubiquitin ligase activity (labelling target proteins for proteasome degradation) and two genes belonging to the proteasome-multienzyme complex itself were analysed by quantitative real-time RT-PCR. The proteasome genes PSMC4 and PSMB5 and the E3 ubiquitin ligase NEDD4L were significantly and coherently upregulated in PCA cells compared with the corresponding adjacent normal prostate tissue. Transcription of the E3 ubiquitin ligase SMURF2 was significantly higher in organ-confined tumours (pT2) compared with non-organ-confined cancers (pT3). The results indicate a role for PSMC4 and PSMB5 and the E3 ubiquitin ligase NEDD4L in prostate tumourigenesis, whereas SMURF2 downregulation could be associated with clinical progression. NEDD4L and SMURF2 both target transforming growth factor (TGF)-? for degradation. This reflects the pleiotropic role of the TGF-? signalling pathway acting as a tumour suppressor in normal and pre-cancerous cells, but having oncogenic properties in progressing cancer. Further studies have to elucidate whether these alterations could represent clinically relevant PCA-diagnostic and progression markers.

M3 - SCORING: Journal article

VL - 14

SP - 38

EP - 45

JO - PROSTATE CANCER P D

JF - PROSTATE CANCER P D

SN - 1365-7852

IS - 1

M1 - 1

ER -