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Timing of antimicrobial prophylaxis for cesarean section is critical for gut microbiome development in term born infants

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Timing of antimicrobial prophylaxis for cesarean section is critical for gut microbiome development in term born infants. / Bossung, Verena; Lupatsii, Mariia; Dashdorj, Lkhagvademberel; Tassiello, Oronzo; Jonassen, Sinje; Pagel, Julia; Demmert, Martin; Wolf, Ellinor Anna; Rody, Achim; Waschina, Silvio; Graspeuntner, Simon; Rupp, Jan; Härtel, Christoph.

In: GUT MICROBES, Vol. 14, No. 1, 2022, p. 2038855.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Bossung, V, Lupatsii, M, Dashdorj, L, Tassiello, O, Jonassen, S, Pagel, J, Demmert, M, Wolf, EA, Rody, A, Waschina, S, Graspeuntner, S, Rupp, J & Härtel, C 2022, 'Timing of antimicrobial prophylaxis for cesarean section is critical for gut microbiome development in term born infants', GUT MICROBES, vol. 14, no. 1, pp. 2038855. https://doi.org/10.1080/19490976.2022.2038855

APA

Bossung, V., Lupatsii, M., Dashdorj, L., Tassiello, O., Jonassen, S., Pagel, J., Demmert, M., Wolf, E. A., Rody, A., Waschina, S., Graspeuntner, S., Rupp, J., & Härtel, C. (2022). Timing of antimicrobial prophylaxis for cesarean section is critical for gut microbiome development in term born infants. GUT MICROBES, 14(1), 2038855. https://doi.org/10.1080/19490976.2022.2038855

Vancouver

Bossung V, Lupatsii M, Dashdorj L, Tassiello O, Jonassen S, Pagel J et al. Timing of antimicrobial prophylaxis for cesarean section is critical for gut microbiome development in term born infants. GUT MICROBES. 2022;14(1):2038855. https://doi.org/10.1080/19490976.2022.2038855

Bibtex

@article{c65a045c10e14aa48178e5b963f5b1c2,
title = "Timing of antimicrobial prophylaxis for cesarean section is critical for gut microbiome development in term born infants",
abstract = "Animal models imply that the perinatal exposure to antibiotics has a substantial impact on microbiome establishment of the offspring. We aimed to evaluate the effect of timing of antimicrobial prophylaxis for cesarean section before versus after cord clamping on gut microbiome composition of term born infants. We performed an exploratory, single center randomized controlled clinical trial. We included forty pregnant women with elective cesarean section at term. The intervention group received single dose intravenous cefuroxime after cord clamping (n = 19), the control group single dose intravenous cefuroxime 30 minutes before skin incision (n = 21). The primary endpoint was microbiome signature of infants and metabolic prediction in the first days of life as determined in meconium samples by 16S rRNA gene sequencing. Secondary endpoints were microbiome composition at one month and 1 year of life. In meconium samples of the intervention group, the genus Staphylococcus pre-dominated. In the control group, the placental cross-over of cefuroxime was confirmed in cord blood. A higher amino acid and nitrogen metabolism as well as increased abundance of the genera Cutibacterium, Corynebacterium and Streptophyta were noted (indicator families: Cytophagaceae, Lactobacilaceae, Oxalobacteraceae). Predictive models of metabolic function revealed higher 2'fucosyllactose utilization in control group samples. In the follow-up visits, a higher abundance of the genus Clostridium was evident in the intervention group. Our exploratory randomized controlled trial suggests that timing of antimicrobial prophylaxis is critical for early microbiome engraftment but not antimicrobial resistance emergence in term born infants.",
keywords = "Anti-Bacterial Agents/pharmacology, Cefuroxime/pharmacology, Cesarean Section/adverse effects, Feces/microbiology, Female, Gastrointestinal Microbiome, Humans, Placenta, Pregnancy, RNA, Ribosomal, 16S/genetics",
author = "Verena Bossung and Mariia Lupatsii and Lkhagvademberel Dashdorj and Oronzo Tassiello and Sinje Jonassen and Julia Pagel and Martin Demmert and Wolf, {Ellinor Anna} and Achim Rody and Silvio Waschina and Simon Graspeuntner and Jan Rupp and Christoph H{\"a}rtel",
year = "2022",
doi = "10.1080/19490976.2022.2038855",
language = "English",
volume = "14",
pages = "2038855",
journal = "GUT MICROBES",
issn = "1949-0976",
publisher = "LANDES BIOSCIENCE",
number = "1",

}

RIS

TY - JOUR

T1 - Timing of antimicrobial prophylaxis for cesarean section is critical for gut microbiome development in term born infants

AU - Bossung, Verena

AU - Lupatsii, Mariia

AU - Dashdorj, Lkhagvademberel

AU - Tassiello, Oronzo

AU - Jonassen, Sinje

AU - Pagel, Julia

AU - Demmert, Martin

AU - Wolf, Ellinor Anna

AU - Rody, Achim

AU - Waschina, Silvio

AU - Graspeuntner, Simon

AU - Rupp, Jan

AU - Härtel, Christoph

PY - 2022

Y1 - 2022

N2 - Animal models imply that the perinatal exposure to antibiotics has a substantial impact on microbiome establishment of the offspring. We aimed to evaluate the effect of timing of antimicrobial prophylaxis for cesarean section before versus after cord clamping on gut microbiome composition of term born infants. We performed an exploratory, single center randomized controlled clinical trial. We included forty pregnant women with elective cesarean section at term. The intervention group received single dose intravenous cefuroxime after cord clamping (n = 19), the control group single dose intravenous cefuroxime 30 minutes before skin incision (n = 21). The primary endpoint was microbiome signature of infants and metabolic prediction in the first days of life as determined in meconium samples by 16S rRNA gene sequencing. Secondary endpoints were microbiome composition at one month and 1 year of life. In meconium samples of the intervention group, the genus Staphylococcus pre-dominated. In the control group, the placental cross-over of cefuroxime was confirmed in cord blood. A higher amino acid and nitrogen metabolism as well as increased abundance of the genera Cutibacterium, Corynebacterium and Streptophyta were noted (indicator families: Cytophagaceae, Lactobacilaceae, Oxalobacteraceae). Predictive models of metabolic function revealed higher 2'fucosyllactose utilization in control group samples. In the follow-up visits, a higher abundance of the genus Clostridium was evident in the intervention group. Our exploratory randomized controlled trial suggests that timing of antimicrobial prophylaxis is critical for early microbiome engraftment but not antimicrobial resistance emergence in term born infants.

AB - Animal models imply that the perinatal exposure to antibiotics has a substantial impact on microbiome establishment of the offspring. We aimed to evaluate the effect of timing of antimicrobial prophylaxis for cesarean section before versus after cord clamping on gut microbiome composition of term born infants. We performed an exploratory, single center randomized controlled clinical trial. We included forty pregnant women with elective cesarean section at term. The intervention group received single dose intravenous cefuroxime after cord clamping (n = 19), the control group single dose intravenous cefuroxime 30 minutes before skin incision (n = 21). The primary endpoint was microbiome signature of infants and metabolic prediction in the first days of life as determined in meconium samples by 16S rRNA gene sequencing. Secondary endpoints were microbiome composition at one month and 1 year of life. In meconium samples of the intervention group, the genus Staphylococcus pre-dominated. In the control group, the placental cross-over of cefuroxime was confirmed in cord blood. A higher amino acid and nitrogen metabolism as well as increased abundance of the genera Cutibacterium, Corynebacterium and Streptophyta were noted (indicator families: Cytophagaceae, Lactobacilaceae, Oxalobacteraceae). Predictive models of metabolic function revealed higher 2'fucosyllactose utilization in control group samples. In the follow-up visits, a higher abundance of the genus Clostridium was evident in the intervention group. Our exploratory randomized controlled trial suggests that timing of antimicrobial prophylaxis is critical for early microbiome engraftment but not antimicrobial resistance emergence in term born infants.

KW - Anti-Bacterial Agents/pharmacology

KW - Cefuroxime/pharmacology

KW - Cesarean Section/adverse effects

KW - Feces/microbiology

KW - Female

KW - Gastrointestinal Microbiome

KW - Humans

KW - Placenta

KW - Pregnancy

KW - RNA, Ribosomal, 16S/genetics

U2 - 10.1080/19490976.2022.2038855

DO - 10.1080/19490976.2022.2038855

M3 - SCORING: Journal article

C2 - 35184691

VL - 14

SP - 2038855

JO - GUT MICROBES

JF - GUT MICROBES

SN - 1949-0976

IS - 1

ER -