Thyroid-stimulating hormone, a novel, locally produced modulator of human epidermal functions, is regulated by thyrotropin-releasing hormone and thyroid hormones
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Thyroid-stimulating hormone, a novel, locally produced modulator of human epidermal functions, is regulated by thyrotropin-releasing hormone and thyroid hormones. / Bodó, Eniko; Kany, Benedikt; Gáspár, Erzsébet; Knüver, Jana; Kromminga, Arno; Ramot, Yuval; Bíró, Tamás; Tiede, Stephan; van Beek, Nina; Poeggeler, Burkhard; Meyer, Katja C; Wenzel, Björn E; Paus, Ralf.
In: ENDOCRINOLOGY, Vol. 151, No. 4, 04.2010, p. 1633-42.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Thyroid-stimulating hormone, a novel, locally produced modulator of human epidermal functions, is regulated by thyrotropin-releasing hormone and thyroid hormones
AU - Bodó, Eniko
AU - Kany, Benedikt
AU - Gáspár, Erzsébet
AU - Knüver, Jana
AU - Kromminga, Arno
AU - Ramot, Yuval
AU - Bíró, Tamás
AU - Tiede, Stephan
AU - van Beek, Nina
AU - Poeggeler, Burkhard
AU - Meyer, Katja C
AU - Wenzel, Björn E
AU - Paus, Ralf
PY - 2010/4
Y1 - 2010/4
N2 - Several elements of the hypothalamic-pituitary-thyroid axis (HPT) reportedly are transcribed by human skin cell populations, and human hair follicles express functional receptors for TSH. Therefore, we asked whether the epidermis of normal human skin is yet another extrathyroidal target of TSH and whether epidermis even produces TSH. If so, we wanted to clarify whether intraepidermal TSH expression is regulated by TRH and/or thyroid hormones and whether TSH alters selected functions of normal human epidermis in situ. TSH and TSH receptor (TSH-R) expression were analyzed in the epidermis of normal human scalp skin by immunohistochemistry and PCR. In addition, full-thickness scalp skin was organ cultured and treated with TSH, TRH, or thyroid hormones, and the effect of TSH treatment on the expression of selected genes was measured by quantitative PCR and/or quantitative immunohistochemistry. Here we show that normal human epidermis expresses TSH at the mRNA and protein levels in situ and transcribes TSH-R. It also contains thyrostimulin transcripts. Intraepidermal TSH immunoreactivity is up-regulated by TRH and down-regulated by thyroid hormones. Although TSH-R immunoreactivity in situ could not be documented within the epidermis, but in the immediately adjacent dermis, TSH treatment of organ-cultured human skin strongly up-regulated epidermal expression of involucrin, loricrin, and keratins 5 and 14. Thus, normal human epidermis in situ is both an extrapituitary source and (possibly an indirect) target of TSH signaling, which regulates defined epidermal parameters. Intraepidermal TSH expression appears to be regulated by the classical endocrine controls that determine the systemic HPT axis.
AB - Several elements of the hypothalamic-pituitary-thyroid axis (HPT) reportedly are transcribed by human skin cell populations, and human hair follicles express functional receptors for TSH. Therefore, we asked whether the epidermis of normal human skin is yet another extrathyroidal target of TSH and whether epidermis even produces TSH. If so, we wanted to clarify whether intraepidermal TSH expression is regulated by TRH and/or thyroid hormones and whether TSH alters selected functions of normal human epidermis in situ. TSH and TSH receptor (TSH-R) expression were analyzed in the epidermis of normal human scalp skin by immunohistochemistry and PCR. In addition, full-thickness scalp skin was organ cultured and treated with TSH, TRH, or thyroid hormones, and the effect of TSH treatment on the expression of selected genes was measured by quantitative PCR and/or quantitative immunohistochemistry. Here we show that normal human epidermis expresses TSH at the mRNA and protein levels in situ and transcribes TSH-R. It also contains thyrostimulin transcripts. Intraepidermal TSH immunoreactivity is up-regulated by TRH and down-regulated by thyroid hormones. Although TSH-R immunoreactivity in situ could not be documented within the epidermis, but in the immediately adjacent dermis, TSH treatment of organ-cultured human skin strongly up-regulated epidermal expression of involucrin, loricrin, and keratins 5 and 14. Thus, normal human epidermis in situ is both an extrapituitary source and (possibly an indirect) target of TSH signaling, which regulates defined epidermal parameters. Intraepidermal TSH expression appears to be regulated by the classical endocrine controls that determine the systemic HPT axis.
KW - Adult
KW - Aged
KW - Apoptosis
KW - Down-Regulation
KW - Epidermis
KW - Female
KW - Humans
KW - Hypothalamo-Hypophyseal System
KW - Immunohistochemistry
KW - Keratin-14
KW - Keratin-5
KW - Membrane Proteins
KW - Middle Aged
KW - Organ Culture Techniques
KW - Protein Precursors
KW - RNA, Messenger
KW - Receptors, Thyrotropin
KW - Reverse Transcriptase Polymerase Chain Reaction
KW - Scalp
KW - Statistics, Nonparametric
KW - Thyrotropin
KW - Thyrotropin-Releasing Hormone
KW - Thyroxine
KW - Triiodothyronine
KW - Up-Regulation
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1210/en.2009-0306
DO - 10.1210/en.2009-0306
M3 - SCORING: Journal article
C2 - 20176727
VL - 151
SP - 1633
EP - 1642
JO - ENDOCRINOLOGY
JF - ENDOCRINOLOGY
SN - 0013-7227
IS - 4
ER -
