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The use of urinary proteomics in the assessment of suitability of mouse models for ageing

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The use of urinary proteomics in the assessment of suitability of mouse models for ageing. / Nkuipou-Kenfack, Esther; Schanstra, Joost P; Bajwa, Seerat; Pejchinovski, Martin; Vinel, Claire; Dray, Cédric; Valet, Philippe; Bascands, Jean-Loup; Vlahou, Antonia; Koeck, Thomas; Borries, Melanie; Busch, Hauke; Bechtel-Walz, Wibke; Huber, Tobias B; Rudolph, Karl L; Pich, Andreas; Mischak, Harald; Zürbig, Petra.

In: PLOS ONE, Vol. 12, No. 2, 2017, p. e0166875.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Nkuipou-Kenfack, E, Schanstra, JP, Bajwa, S, Pejchinovski, M, Vinel, C, Dray, C, Valet, P, Bascands, J-L, Vlahou, A, Koeck, T, Borries, M, Busch, H, Bechtel-Walz, W, Huber, TB, Rudolph, KL, Pich, A, Mischak, H & Zürbig, P 2017, 'The use of urinary proteomics in the assessment of suitability of mouse models for ageing', PLOS ONE, vol. 12, no. 2, pp. e0166875. https://doi.org/10.1371/journal.pone.0166875

APA

Nkuipou-Kenfack, E., Schanstra, J. P., Bajwa, S., Pejchinovski, M., Vinel, C., Dray, C., Valet, P., Bascands, J-L., Vlahou, A., Koeck, T., Borries, M., Busch, H., Bechtel-Walz, W., Huber, T. B., Rudolph, K. L., Pich, A., Mischak, H., & Zürbig, P. (2017). The use of urinary proteomics in the assessment of suitability of mouse models for ageing. PLOS ONE, 12(2), e0166875. https://doi.org/10.1371/journal.pone.0166875

Vancouver

Nkuipou-Kenfack E, Schanstra JP, Bajwa S, Pejchinovski M, Vinel C, Dray C et al. The use of urinary proteomics in the assessment of suitability of mouse models for ageing. PLOS ONE. 2017;12(2):e0166875. https://doi.org/10.1371/journal.pone.0166875

Bibtex

@article{40acd654de0e4962946e5a01cf4b73ac,
title = "The use of urinary proteomics in the assessment of suitability of mouse models for ageing",
abstract = "Ageing is a complex process characterised by a systemic and progressive deterioration of biological functions. As ageing is associated with an increased prevalence of age-related chronic disorders, understanding its underlying molecular mechanisms can pave the way for therapeutic interventions and managing complications. Animal models such as mice are commonly used in ageing research as they have a shorter lifespan in comparison to humans and are also genetically close to humans. To assess the translatability of mouse ageing to human ageing, the urinary proteome in 89 wild-type (C57BL/6) mice aged between 8-96 weeks was investigated using capillary electrophoresis coupled to mass spectrometry (CE-MS). Using age as a continuous variable, 295 peptides significantly correlated with age in mice were identified. To investigate the relevance of using mouse models in human ageing studies, a comparison was performed with a previous correlation analysis using 1227 healthy subjects. In mice and humans, a decrease in urinary excretion of fibrillar collagens and an increase of uromodulin fragments was observed with advanced age. Of the 295 peptides correlating with age, 49 had a strong homology to the respective human age-related peptides. These ortholog peptides including several collagen (N = 44) and uromodulin (N = 5) fragments were used to generate an ageing classifier that was able to discriminate the age among both wild-type mice and healthy subjects. Additionally, the ageing classifier depicted that telomerase knock-out mice were older than their chronological age. Hence, with a focus on ortholog urinary peptides mouse ageing can be translated to human ageing.",
keywords = "Aging, Animals, Capillary Electrochromatography, Female, Humans, Male, Mass Spectrometry, Mice, Mice, Knockout, Models, Biological, Peptides, Proteome, Proteomics, Journal Article",
author = "Esther Nkuipou-Kenfack and Schanstra, {Joost P} and Seerat Bajwa and Martin Pejchinovski and Claire Vinel and C{\'e}dric Dray and Philippe Valet and Jean-Loup Bascands and Antonia Vlahou and Thomas Koeck and Melanie Borries and Hauke Busch and Wibke Bechtel-Walz and Huber, {Tobias B} and Rudolph, {Karl L} and Andreas Pich and Harald Mischak and Petra Z{\"u}rbig",
year = "2017",
doi = "10.1371/journal.pone.0166875",
language = "English",
volume = "12",
pages = "e0166875",
journal = "PLOS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "2",

}

RIS

TY - JOUR

T1 - The use of urinary proteomics in the assessment of suitability of mouse models for ageing

AU - Nkuipou-Kenfack, Esther

AU - Schanstra, Joost P

AU - Bajwa, Seerat

AU - Pejchinovski, Martin

AU - Vinel, Claire

AU - Dray, Cédric

AU - Valet, Philippe

AU - Bascands, Jean-Loup

AU - Vlahou, Antonia

AU - Koeck, Thomas

AU - Borries, Melanie

AU - Busch, Hauke

AU - Bechtel-Walz, Wibke

AU - Huber, Tobias B

AU - Rudolph, Karl L

AU - Pich, Andreas

AU - Mischak, Harald

AU - Zürbig, Petra

PY - 2017

Y1 - 2017

N2 - Ageing is a complex process characterised by a systemic and progressive deterioration of biological functions. As ageing is associated with an increased prevalence of age-related chronic disorders, understanding its underlying molecular mechanisms can pave the way for therapeutic interventions and managing complications. Animal models such as mice are commonly used in ageing research as they have a shorter lifespan in comparison to humans and are also genetically close to humans. To assess the translatability of mouse ageing to human ageing, the urinary proteome in 89 wild-type (C57BL/6) mice aged between 8-96 weeks was investigated using capillary electrophoresis coupled to mass spectrometry (CE-MS). Using age as a continuous variable, 295 peptides significantly correlated with age in mice were identified. To investigate the relevance of using mouse models in human ageing studies, a comparison was performed with a previous correlation analysis using 1227 healthy subjects. In mice and humans, a decrease in urinary excretion of fibrillar collagens and an increase of uromodulin fragments was observed with advanced age. Of the 295 peptides correlating with age, 49 had a strong homology to the respective human age-related peptides. These ortholog peptides including several collagen (N = 44) and uromodulin (N = 5) fragments were used to generate an ageing classifier that was able to discriminate the age among both wild-type mice and healthy subjects. Additionally, the ageing classifier depicted that telomerase knock-out mice were older than their chronological age. Hence, with a focus on ortholog urinary peptides mouse ageing can be translated to human ageing.

AB - Ageing is a complex process characterised by a systemic and progressive deterioration of biological functions. As ageing is associated with an increased prevalence of age-related chronic disorders, understanding its underlying molecular mechanisms can pave the way for therapeutic interventions and managing complications. Animal models such as mice are commonly used in ageing research as they have a shorter lifespan in comparison to humans and are also genetically close to humans. To assess the translatability of mouse ageing to human ageing, the urinary proteome in 89 wild-type (C57BL/6) mice aged between 8-96 weeks was investigated using capillary electrophoresis coupled to mass spectrometry (CE-MS). Using age as a continuous variable, 295 peptides significantly correlated with age in mice were identified. To investigate the relevance of using mouse models in human ageing studies, a comparison was performed with a previous correlation analysis using 1227 healthy subjects. In mice and humans, a decrease in urinary excretion of fibrillar collagens and an increase of uromodulin fragments was observed with advanced age. Of the 295 peptides correlating with age, 49 had a strong homology to the respective human age-related peptides. These ortholog peptides including several collagen (N = 44) and uromodulin (N = 5) fragments were used to generate an ageing classifier that was able to discriminate the age among both wild-type mice and healthy subjects. Additionally, the ageing classifier depicted that telomerase knock-out mice were older than their chronological age. Hence, with a focus on ortholog urinary peptides mouse ageing can be translated to human ageing.

KW - Aging

KW - Animals

KW - Capillary Electrochromatography

KW - Female

KW - Humans

KW - Male

KW - Mass Spectrometry

KW - Mice

KW - Mice, Knockout

KW - Models, Biological

KW - Peptides

KW - Proteome

KW - Proteomics

KW - Journal Article

U2 - 10.1371/journal.pone.0166875

DO - 10.1371/journal.pone.0166875

M3 - SCORING: Journal article

C2 - 28199320

VL - 12

SP - e0166875

JO - PLOS ONE

JF - PLOS ONE

SN - 1932-6203

IS - 2

ER -