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The ubiquitin-conjugating enzyme UBE2K determines neurogenic potential through histone H3 in human embryonic stem cells

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The ubiquitin-conjugating enzyme UBE2K determines neurogenic potential through histone H3 in human embryonic stem cells. / Fatima, Azra; Irmak, Dilber; Noormohammadi, Alireza; Rinschen, Markus M; Das, Aniruddha; Leidecker, Orsolya; Schindler, Christina; Sánchez-Gaya, Víctor; Wagle, Prerana; Pokrzywa, Wojciech; Hoppe, Thorsten; Rada-Iglesias, Alvaro; Vilchez, David.

In: COMMUN BIOL, Vol. 3, No. 1, 25.05.2020, p. 262.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Fatima, A, Irmak, D, Noormohammadi, A, Rinschen, MM, Das, A, Leidecker, O, Schindler, C, Sánchez-Gaya, V, Wagle, P, Pokrzywa, W, Hoppe, T, Rada-Iglesias, A & Vilchez, D 2020, 'The ubiquitin-conjugating enzyme UBE2K determines neurogenic potential through histone H3 in human embryonic stem cells', COMMUN BIOL, vol. 3, no. 1, pp. 262. https://doi.org/10.1038/s42003-020-0984-3

APA

Fatima, A., Irmak, D., Noormohammadi, A., Rinschen, M. M., Das, A., Leidecker, O., Schindler, C., Sánchez-Gaya, V., Wagle, P., Pokrzywa, W., Hoppe, T., Rada-Iglesias, A., & Vilchez, D. (2020). The ubiquitin-conjugating enzyme UBE2K determines neurogenic potential through histone H3 in human embryonic stem cells. COMMUN BIOL, 3(1), 262. https://doi.org/10.1038/s42003-020-0984-3

Vancouver

Fatima A, Irmak D, Noormohammadi A, Rinschen MM, Das A, Leidecker O et al. The ubiquitin-conjugating enzyme UBE2K determines neurogenic potential through histone H3 in human embryonic stem cells. COMMUN BIOL. 2020 May 25;3(1):262. https://doi.org/10.1038/s42003-020-0984-3

Bibtex

@article{462840a7f30f4e268773faefd8adeb5e,
title = "The ubiquitin-conjugating enzyme UBE2K determines neurogenic potential through histone H3 in human embryonic stem cells",
abstract = "Histones modulate gene expression by chromatin compaction, regulating numerous processes such as differentiation. However, the mechanisms underlying histone degradation remain elusive. Human embryonic stem cells (hESCs) have a unique chromatin architecture characterized by low levels of trimethylated histone H3 at lysine 9 (H3K9me3), a heterochromatin-associated modification. Here we assess the link between the intrinsic epigenetic landscape and ubiquitin-proteasome system of hESCs. We find that hESCs exhibit high expression of the ubiquitin-conjugating enzyme UBE2K. Loss of UBE2K upregulates the trimethyltransferase SETDB1, resulting in H3K9 trimethylation and repression of neurogenic genes during differentiation. Besides H3K9 trimethylation, UBE2K binds histone H3 to induce its polyubiquitination and degradation by the proteasome. Notably, ubc-20, the worm orthologue of UBE2K, also regulates histone H3 levels and H3K9 trimethylation in Caenorhabditis elegans germ cells. Thus, our results indicate that UBE2K crosses evolutionary boundaries to promote histone H3 degradation and reduce H3K9me3 repressive marks in immortal cells.",
author = "Azra Fatima and Dilber Irmak and Alireza Noormohammadi and Rinschen, {Markus M} and Aniruddha Das and Orsolya Leidecker and Christina Schindler and V{\'i}ctor S{\'a}nchez-Gaya and Prerana Wagle and Wojciech Pokrzywa and Thorsten Hoppe and Alvaro Rada-Iglesias and David Vilchez",
year = "2020",
month = may,
day = "25",
doi = "10.1038/s42003-020-0984-3",
language = "English",
volume = "3",
pages = "262",
journal = "COMMUN BIOL",
issn = "2399-3642",
publisher = "NATURE PUBLISHING GROUP",
number = "1",

}

RIS

TY - JOUR

T1 - The ubiquitin-conjugating enzyme UBE2K determines neurogenic potential through histone H3 in human embryonic stem cells

AU - Fatima, Azra

AU - Irmak, Dilber

AU - Noormohammadi, Alireza

AU - Rinschen, Markus M

AU - Das, Aniruddha

AU - Leidecker, Orsolya

AU - Schindler, Christina

AU - Sánchez-Gaya, Víctor

AU - Wagle, Prerana

AU - Pokrzywa, Wojciech

AU - Hoppe, Thorsten

AU - Rada-Iglesias, Alvaro

AU - Vilchez, David

PY - 2020/5/25

Y1 - 2020/5/25

N2 - Histones modulate gene expression by chromatin compaction, regulating numerous processes such as differentiation. However, the mechanisms underlying histone degradation remain elusive. Human embryonic stem cells (hESCs) have a unique chromatin architecture characterized by low levels of trimethylated histone H3 at lysine 9 (H3K9me3), a heterochromatin-associated modification. Here we assess the link between the intrinsic epigenetic landscape and ubiquitin-proteasome system of hESCs. We find that hESCs exhibit high expression of the ubiquitin-conjugating enzyme UBE2K. Loss of UBE2K upregulates the trimethyltransferase SETDB1, resulting in H3K9 trimethylation and repression of neurogenic genes during differentiation. Besides H3K9 trimethylation, UBE2K binds histone H3 to induce its polyubiquitination and degradation by the proteasome. Notably, ubc-20, the worm orthologue of UBE2K, also regulates histone H3 levels and H3K9 trimethylation in Caenorhabditis elegans germ cells. Thus, our results indicate that UBE2K crosses evolutionary boundaries to promote histone H3 degradation and reduce H3K9me3 repressive marks in immortal cells.

AB - Histones modulate gene expression by chromatin compaction, regulating numerous processes such as differentiation. However, the mechanisms underlying histone degradation remain elusive. Human embryonic stem cells (hESCs) have a unique chromatin architecture characterized by low levels of trimethylated histone H3 at lysine 9 (H3K9me3), a heterochromatin-associated modification. Here we assess the link between the intrinsic epigenetic landscape and ubiquitin-proteasome system of hESCs. We find that hESCs exhibit high expression of the ubiquitin-conjugating enzyme UBE2K. Loss of UBE2K upregulates the trimethyltransferase SETDB1, resulting in H3K9 trimethylation and repression of neurogenic genes during differentiation. Besides H3K9 trimethylation, UBE2K binds histone H3 to induce its polyubiquitination and degradation by the proteasome. Notably, ubc-20, the worm orthologue of UBE2K, also regulates histone H3 levels and H3K9 trimethylation in Caenorhabditis elegans germ cells. Thus, our results indicate that UBE2K crosses evolutionary boundaries to promote histone H3 degradation and reduce H3K9me3 repressive marks in immortal cells.

U2 - 10.1038/s42003-020-0984-3

DO - 10.1038/s42003-020-0984-3

M3 - SCORING: Journal article

C2 - 32451438

VL - 3

SP - 262

JO - COMMUN BIOL

JF - COMMUN BIOL

SN - 2399-3642

IS - 1

ER -