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The T-cell receptor associated zeta-chain is required but not sufficient for CD26 (dipeptidylpeptidase IV) mediated signaling

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The T-cell receptor associated zeta-chain is required but not sufficient for CD26 (dipeptidylpeptidase IV) mediated signaling. / von Bonin, A; Steeg, C; Mittrücker, H W; Fleischer, B.

In: IMMUNOL LETT, Vol. 55, No. 3, 01.03.1997, p. 179-82.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

von Bonin, A, Steeg, C, Mittrücker, HW & Fleischer, B 1997, 'The T-cell receptor associated zeta-chain is required but not sufficient for CD26 (dipeptidylpeptidase IV) mediated signaling', IMMUNOL LETT, vol. 55, no. 3, pp. 179-82.

APA

von Bonin, A., Steeg, C., Mittrücker, H. W., & Fleischer, B. (1997). The T-cell receptor associated zeta-chain is required but not sufficient for CD26 (dipeptidylpeptidase IV) mediated signaling. IMMUNOL LETT, 55(3), 179-82.

Vancouver

von Bonin A, Steeg C, Mittrücker HW, Fleischer B. The T-cell receptor associated zeta-chain is required but not sufficient for CD26 (dipeptidylpeptidase IV) mediated signaling. IMMUNOL LETT. 1997 Mar 1;55(3):179-82.

Bibtex

@article{66c43c6cabb24d7fa1e63d8d4cb60899,
title = "The T-cell receptor associated zeta-chain is required but not sufficient for CD26 (dipeptidylpeptidase IV) mediated signaling",
abstract = "CD26 or dipeptidylpeptidase IV (DPP IV) is a cell surface protease involved in T-cell activation. Triggering or costimulation of T-cells via CD26 was shown to be dependent on the expression of the T-cell receptor (TCR) associated zeta-chain with at least one functional immune receptor tyrosine based activation motif (ITAM). Here we tested T-cell lines expressing chimeric proteins (hCD25-zeta) consisting of human IL-2 receptor-alpha chain derived extracellular sequences (hCD25) fused to mouse-specific zeta-chain segments, for their capacity to transfer CD26 mediated signals. Although these 'minimal receptor' expressing T-cell lines were capable of transmitting signals from other costimulatory molecules (e.g. CD2), crosslinking of CD26 did not induce IL-2 secretion. Co-cross-linking of hCD25 and CD26 molecules, however, resulted in the stimulation of the T-cells. Thus, although the zeta-chain is a prerequisite for CD26 mediated signaling events, the sole expression of zeta-protein as a signaling molecule is not sufficient for CD26 mediated triggering but permits CD26 induced costimulation in TCR negative cells.",
keywords = "Cells, Cultured, Dipeptidyl Peptidase 4, Humans, Interleukin-2, Membrane Proteins, Radioimmunoassay, Receptors, Antigen, T-Cell, Receptors, Interleukin-2, Recombinant Proteins, Signal Transduction, Transfection",
author = "{von Bonin}, A and C Steeg and Mittr{\"u}cker, {H W} and B Fleischer",
year = "1997",
month = mar,
day = "1",
language = "English",
volume = "55",
pages = "179--82",
journal = "IMMUNOL LETT",
issn = "0165-2478",
publisher = "Elsevier",
number = "3",

}

RIS

TY - JOUR

T1 - The T-cell receptor associated zeta-chain is required but not sufficient for CD26 (dipeptidylpeptidase IV) mediated signaling

AU - von Bonin, A

AU - Steeg, C

AU - Mittrücker, H W

AU - Fleischer, B

PY - 1997/3/1

Y1 - 1997/3/1

N2 - CD26 or dipeptidylpeptidase IV (DPP IV) is a cell surface protease involved in T-cell activation. Triggering or costimulation of T-cells via CD26 was shown to be dependent on the expression of the T-cell receptor (TCR) associated zeta-chain with at least one functional immune receptor tyrosine based activation motif (ITAM). Here we tested T-cell lines expressing chimeric proteins (hCD25-zeta) consisting of human IL-2 receptor-alpha chain derived extracellular sequences (hCD25) fused to mouse-specific zeta-chain segments, for their capacity to transfer CD26 mediated signals. Although these 'minimal receptor' expressing T-cell lines were capable of transmitting signals from other costimulatory molecules (e.g. CD2), crosslinking of CD26 did not induce IL-2 secretion. Co-cross-linking of hCD25 and CD26 molecules, however, resulted in the stimulation of the T-cells. Thus, although the zeta-chain is a prerequisite for CD26 mediated signaling events, the sole expression of zeta-protein as a signaling molecule is not sufficient for CD26 mediated triggering but permits CD26 induced costimulation in TCR negative cells.

AB - CD26 or dipeptidylpeptidase IV (DPP IV) is a cell surface protease involved in T-cell activation. Triggering or costimulation of T-cells via CD26 was shown to be dependent on the expression of the T-cell receptor (TCR) associated zeta-chain with at least one functional immune receptor tyrosine based activation motif (ITAM). Here we tested T-cell lines expressing chimeric proteins (hCD25-zeta) consisting of human IL-2 receptor-alpha chain derived extracellular sequences (hCD25) fused to mouse-specific zeta-chain segments, for their capacity to transfer CD26 mediated signals. Although these 'minimal receptor' expressing T-cell lines were capable of transmitting signals from other costimulatory molecules (e.g. CD2), crosslinking of CD26 did not induce IL-2 secretion. Co-cross-linking of hCD25 and CD26 molecules, however, resulted in the stimulation of the T-cells. Thus, although the zeta-chain is a prerequisite for CD26 mediated signaling events, the sole expression of zeta-protein as a signaling molecule is not sufficient for CD26 mediated triggering but permits CD26 induced costimulation in TCR negative cells.

KW - Cells, Cultured

KW - Dipeptidyl Peptidase 4

KW - Humans

KW - Interleukin-2

KW - Membrane Proteins

KW - Radioimmunoassay

KW - Receptors, Antigen, T-Cell

KW - Receptors, Interleukin-2

KW - Recombinant Proteins

KW - Signal Transduction

KW - Transfection

M3 - SCORING: Journal article

C2 - 9161885

VL - 55

SP - 179

EP - 182

JO - IMMUNOL LETT

JF - IMMUNOL LETT

SN - 0165-2478

IS - 3

ER -