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The sperm protamine mRNA ratio as a clinical parameter to estimate the fertilizing potential of men taking part in an ART programme

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The sperm protamine mRNA ratio as a clinical parameter to estimate the fertilizing potential of men taking part in an ART programme. / Rogenhofer, Nina; Dansranjavin, Temuujin; Schorsch, Martin; Spiess, Andrej-Nikolai; Wang, Hongxiang; von Schönfeldt, Viktoria; Cappallo-Obermann, Heike; Baukloh, Vera; Yang, Hao; Paradowska, Agnieszka; Chen, Bin; Thaler, Christian J; Weidner, Wolfgang; Schuppe, Hans-Christian; Steger, Klaus.

In: HUM REPROD, Vol. 28, No. 4, 01.04.2013, p. 969-78.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Rogenhofer, N, Dansranjavin, T, Schorsch, M, Spiess, A-N, Wang, H, von Schönfeldt, V, Cappallo-Obermann, H, Baukloh, V, Yang, H, Paradowska, A, Chen, B, Thaler, CJ, Weidner, W, Schuppe, H-C & Steger, K 2013, 'The sperm protamine mRNA ratio as a clinical parameter to estimate the fertilizing potential of men taking part in an ART programme', HUM REPROD, vol. 28, no. 4, pp. 969-78. https://doi.org/10.1093/humrep/des471

APA

Rogenhofer, N., Dansranjavin, T., Schorsch, M., Spiess, A-N., Wang, H., von Schönfeldt, V., Cappallo-Obermann, H., Baukloh, V., Yang, H., Paradowska, A., Chen, B., Thaler, C. J., Weidner, W., Schuppe, H-C., & Steger, K. (2013). The sperm protamine mRNA ratio as a clinical parameter to estimate the fertilizing potential of men taking part in an ART programme. HUM REPROD, 28(4), 969-78. https://doi.org/10.1093/humrep/des471

Vancouver

Rogenhofer N, Dansranjavin T, Schorsch M, Spiess A-N, Wang H, von Schönfeldt V et al. The sperm protamine mRNA ratio as a clinical parameter to estimate the fertilizing potential of men taking part in an ART programme. HUM REPROD. 2013 Apr 1;28(4):969-78. https://doi.org/10.1093/humrep/des471

Bibtex

@article{6d83874ee64b492b8156ad7090c3a7e3,
title = "The sperm protamine mRNA ratio as a clinical parameter to estimate the fertilizing potential of men taking part in an ART programme",
abstract = "STUDY QUESTION: Could the protamine-1 to protamine-2 mRNA ratio serve as a biomarker to estimate the fertilizing capacity of sperm from men taking part in an IVF/ICSI programme?SUMMARY ANSWER: The protamine mRNA ratio clearly discriminates between fertile and subfertile men and sperm with a normal protamine mRNA ratio exhibit a higher fertilizing capacity in IVF/ICSI.WHAT IS KNOWN ALREADY: Aberrant sperm protamine ratios are associated with male factor infertility and mRNA ratio is comparable with protein ratio (due to transcriptional stop in elongating spermatids).STUDY DESIGN, SIZE, DURATION: The study population was drawn from subfertile men, whose female partners participated in IVF or ICSI programmes between September 2010 and February 2012. Normozoospermic healthy volunteers served as controls. Sperm cells were lysed, mRNA extracted, reverse transcribed and subjected to real-time quantitative PCR using specific primer pairs for protamine-1 and protamine-2. Relative protamine-1 and protamine-2 mRNA levels were analysed with the Mann-Whitney U-test (two-tailed).PARTICIPANTS/MATERIALS, SETTING, METHODS: Quantitative RT-PCR for protamines 1 and 2 has been performed in ejaculates from 32 normozoospermic volunteers (control, University Clinic Giessen, Germany) and 306 patients, whose female partners took part in an IVF (n = 76; University Clinic Hamburg, Germany and Shanghai Jiaotong University, China) or an ICSI (n = 230; University Clinic Munich, Germany and Kinderwunschzentrum Wiesbaden, Germany) programme.MAIN RESULTS AND THE ROLE OF CHANCE: The sperm protamine mRNA ratio in normozoospermic men (0.98 ± 0.3) differed significantly from that of ICSI patients (Munich 0.81 ± 0.1; Wiesbaden 0.78 ± 0.2; P < 0.001), while processed samples obtained from IVF patients revealed a normal protamine mRNA ratio (Hamburg 1.0 ± 0.07; Shanghai 1.0 ± 0.54). Normal protamine mRNA ratios were associated with a significantly higher total motile sperm count and a significantly higher percentage of progressively motile sperm. Sperm with a normal protamine mRNA ratio revealed a higher fertilization capacity (fc) in both IVF (53.6% of patients with fc > 80%; P = 0.017) and ICSI (65.1% of patients with fc > 70%; P = 0.028).LIMITATIONS, REASONS FOR CAUTION: The protamine mRNA ratio in an individual sperm cell used for ICSI may be different from the overall value obtained from a semen aliquot.WIDER IMPLICATIONS OF THE FINDINGS: Data are in line with current literature and suggest the protamine mRNA ratio as a diagnostic marker to estimate the fertilizing capacity of sperm.STUDY FUNDING: The German Research Foundation (DFG) to K.S., W.W. and A.P. (STE 892/9-2), as well as to A.S. and H.C.O. (SP721/1-3).COMPETING INTEREST(S): None.",
keywords = "Adult, Biological Markers, Female, Fertilization, Fertilization in Vitro, Humans, Infertility, Male, Male, Pregnancy, Pregnancy Outcome, Pregnancy Rate, Protamines, RNA, Messenger, Sperm Injections, Intracytoplasmic, Sperm-Ovum Interactions, Spermatozoa",
author = "Nina Rogenhofer and Temuujin Dansranjavin and Martin Schorsch and Andrej-Nikolai Spiess and Hongxiang Wang and {von Sch{\"o}nfeldt}, Viktoria and Heike Cappallo-Obermann and Vera Baukloh and Hao Yang and Agnieszka Paradowska and Bin Chen and Thaler, {Christian J} and Wolfgang Weidner and Hans-Christian Schuppe and Klaus Steger",
year = "2013",
month = apr,
day = "1",
doi = "10.1093/humrep/des471",
language = "English",
volume = "28",
pages = "969--78",
journal = "HUM REPROD",
issn = "0268-1161",
publisher = "Oxford University Press",
number = "4",

}

RIS

TY - JOUR

T1 - The sperm protamine mRNA ratio as a clinical parameter to estimate the fertilizing potential of men taking part in an ART programme

AU - Rogenhofer, Nina

AU - Dansranjavin, Temuujin

AU - Schorsch, Martin

AU - Spiess, Andrej-Nikolai

AU - Wang, Hongxiang

AU - von Schönfeldt, Viktoria

AU - Cappallo-Obermann, Heike

AU - Baukloh, Vera

AU - Yang, Hao

AU - Paradowska, Agnieszka

AU - Chen, Bin

AU - Thaler, Christian J

AU - Weidner, Wolfgang

AU - Schuppe, Hans-Christian

AU - Steger, Klaus

PY - 2013/4/1

Y1 - 2013/4/1

N2 - STUDY QUESTION: Could the protamine-1 to protamine-2 mRNA ratio serve as a biomarker to estimate the fertilizing capacity of sperm from men taking part in an IVF/ICSI programme?SUMMARY ANSWER: The protamine mRNA ratio clearly discriminates between fertile and subfertile men and sperm with a normal protamine mRNA ratio exhibit a higher fertilizing capacity in IVF/ICSI.WHAT IS KNOWN ALREADY: Aberrant sperm protamine ratios are associated with male factor infertility and mRNA ratio is comparable with protein ratio (due to transcriptional stop in elongating spermatids).STUDY DESIGN, SIZE, DURATION: The study population was drawn from subfertile men, whose female partners participated in IVF or ICSI programmes between September 2010 and February 2012. Normozoospermic healthy volunteers served as controls. Sperm cells were lysed, mRNA extracted, reverse transcribed and subjected to real-time quantitative PCR using specific primer pairs for protamine-1 and protamine-2. Relative protamine-1 and protamine-2 mRNA levels were analysed with the Mann-Whitney U-test (two-tailed).PARTICIPANTS/MATERIALS, SETTING, METHODS: Quantitative RT-PCR for protamines 1 and 2 has been performed in ejaculates from 32 normozoospermic volunteers (control, University Clinic Giessen, Germany) and 306 patients, whose female partners took part in an IVF (n = 76; University Clinic Hamburg, Germany and Shanghai Jiaotong University, China) or an ICSI (n = 230; University Clinic Munich, Germany and Kinderwunschzentrum Wiesbaden, Germany) programme.MAIN RESULTS AND THE ROLE OF CHANCE: The sperm protamine mRNA ratio in normozoospermic men (0.98 ± 0.3) differed significantly from that of ICSI patients (Munich 0.81 ± 0.1; Wiesbaden 0.78 ± 0.2; P < 0.001), while processed samples obtained from IVF patients revealed a normal protamine mRNA ratio (Hamburg 1.0 ± 0.07; Shanghai 1.0 ± 0.54). Normal protamine mRNA ratios were associated with a significantly higher total motile sperm count and a significantly higher percentage of progressively motile sperm. Sperm with a normal protamine mRNA ratio revealed a higher fertilization capacity (fc) in both IVF (53.6% of patients with fc > 80%; P = 0.017) and ICSI (65.1% of patients with fc > 70%; P = 0.028).LIMITATIONS, REASONS FOR CAUTION: The protamine mRNA ratio in an individual sperm cell used for ICSI may be different from the overall value obtained from a semen aliquot.WIDER IMPLICATIONS OF THE FINDINGS: Data are in line with current literature and suggest the protamine mRNA ratio as a diagnostic marker to estimate the fertilizing capacity of sperm.STUDY FUNDING: The German Research Foundation (DFG) to K.S., W.W. and A.P. (STE 892/9-2), as well as to A.S. and H.C.O. (SP721/1-3).COMPETING INTEREST(S): None.

AB - STUDY QUESTION: Could the protamine-1 to protamine-2 mRNA ratio serve as a biomarker to estimate the fertilizing capacity of sperm from men taking part in an IVF/ICSI programme?SUMMARY ANSWER: The protamine mRNA ratio clearly discriminates between fertile and subfertile men and sperm with a normal protamine mRNA ratio exhibit a higher fertilizing capacity in IVF/ICSI.WHAT IS KNOWN ALREADY: Aberrant sperm protamine ratios are associated with male factor infertility and mRNA ratio is comparable with protein ratio (due to transcriptional stop in elongating spermatids).STUDY DESIGN, SIZE, DURATION: The study population was drawn from subfertile men, whose female partners participated in IVF or ICSI programmes between September 2010 and February 2012. Normozoospermic healthy volunteers served as controls. Sperm cells were lysed, mRNA extracted, reverse transcribed and subjected to real-time quantitative PCR using specific primer pairs for protamine-1 and protamine-2. Relative protamine-1 and protamine-2 mRNA levels were analysed with the Mann-Whitney U-test (two-tailed).PARTICIPANTS/MATERIALS, SETTING, METHODS: Quantitative RT-PCR for protamines 1 and 2 has been performed in ejaculates from 32 normozoospermic volunteers (control, University Clinic Giessen, Germany) and 306 patients, whose female partners took part in an IVF (n = 76; University Clinic Hamburg, Germany and Shanghai Jiaotong University, China) or an ICSI (n = 230; University Clinic Munich, Germany and Kinderwunschzentrum Wiesbaden, Germany) programme.MAIN RESULTS AND THE ROLE OF CHANCE: The sperm protamine mRNA ratio in normozoospermic men (0.98 ± 0.3) differed significantly from that of ICSI patients (Munich 0.81 ± 0.1; Wiesbaden 0.78 ± 0.2; P < 0.001), while processed samples obtained from IVF patients revealed a normal protamine mRNA ratio (Hamburg 1.0 ± 0.07; Shanghai 1.0 ± 0.54). Normal protamine mRNA ratios were associated with a significantly higher total motile sperm count and a significantly higher percentage of progressively motile sperm. Sperm with a normal protamine mRNA ratio revealed a higher fertilization capacity (fc) in both IVF (53.6% of patients with fc > 80%; P = 0.017) and ICSI (65.1% of patients with fc > 70%; P = 0.028).LIMITATIONS, REASONS FOR CAUTION: The protamine mRNA ratio in an individual sperm cell used for ICSI may be different from the overall value obtained from a semen aliquot.WIDER IMPLICATIONS OF THE FINDINGS: Data are in line with current literature and suggest the protamine mRNA ratio as a diagnostic marker to estimate the fertilizing capacity of sperm.STUDY FUNDING: The German Research Foundation (DFG) to K.S., W.W. and A.P. (STE 892/9-2), as well as to A.S. and H.C.O. (SP721/1-3).COMPETING INTEREST(S): None.

KW - Adult

KW - Biological Markers

KW - Female

KW - Fertilization

KW - Fertilization in Vitro

KW - Humans

KW - Infertility, Male

KW - Male

KW - Pregnancy

KW - Pregnancy Outcome

KW - Pregnancy Rate

KW - Protamines

KW - RNA, Messenger

KW - Sperm Injections, Intracytoplasmic

KW - Sperm-Ovum Interactions

KW - Spermatozoa

U2 - 10.1093/humrep/des471

DO - 10.1093/humrep/des471

M3 - SCORING: Journal article

C2 - 23340056

VL - 28

SP - 969

EP - 978

JO - HUM REPROD

JF - HUM REPROD

SN - 0268-1161

IS - 4

ER -