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The search for the primary tumor in metastasized gastroenteropancreatic neuroendocrine neoplasm

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The search for the primary tumor in metastasized gastroenteropancreatic neuroendocrine neoplasm. / Kaemmerer, D; Posorski, N; von Eggeling, F; Ernst, G; Hörsch, D; Baum, R P; Prasad, V; Langer, R; Esposito, I; Klöppel, G; Sehner, S; Knösel, T; Hommann, M.

In: CLIN EXP METASTAS, Vol. 31, No. 7, 2014, p. 817-27.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Kaemmerer, D, Posorski, N, von Eggeling, F, Ernst, G, Hörsch, D, Baum, RP, Prasad, V, Langer, R, Esposito, I, Klöppel, G, Sehner, S, Knösel, T & Hommann, M 2014, 'The search for the primary tumor in metastasized gastroenteropancreatic neuroendocrine neoplasm', CLIN EXP METASTAS, vol. 31, no. 7, pp. 817-27. https://doi.org/10.1007/s10585-014-9672-3

APA

Kaemmerer, D., Posorski, N., von Eggeling, F., Ernst, G., Hörsch, D., Baum, R. P., Prasad, V., Langer, R., Esposito, I., Klöppel, G., Sehner, S., Knösel, T., & Hommann, M. (2014). The search for the primary tumor in metastasized gastroenteropancreatic neuroendocrine neoplasm. CLIN EXP METASTAS, 31(7), 817-27. https://doi.org/10.1007/s10585-014-9672-3

Vancouver

Kaemmerer D, Posorski N, von Eggeling F, Ernst G, Hörsch D, Baum RP et al. The search for the primary tumor in metastasized gastroenteropancreatic neuroendocrine neoplasm. CLIN EXP METASTAS. 2014;31(7):817-27. https://doi.org/10.1007/s10585-014-9672-3

Bibtex

@article{f0def44827934e63a5c1ffef6831e1e4,
title = "The search for the primary tumor in metastasized gastroenteropancreatic neuroendocrine neoplasm",
abstract = "Gastroenteropancreatic neuroendocrine tumors (NETs) often present as liver metastasis from a carcinoma of unknown primary. We recently showed that primary NETs from the pancreas, small intestine and stomach as well as their respective liver metastases differ from each other by the expression profile of the three genes CD302, PPWD1 and ABHB14B. The gene and protein expression of CD302, PPWD1, and ABHB14B was studied in abdominal NET metastases to identify the site of the respective primary tumors. Cryopreserved tissue from NET metastases collected in different institutions (group A: 29, group B: 50, group C: 132 specimens) were examined by comparative genomic hybridization (Agilent 105 K), gene expression analysis (Agilent 44 K) (groups A and B) and immunohistochemistry (group C). The data were blindly evaluated, i.e. without knowing the site of the primary. Gene expression analysis correctly revealed the primary in the ileum in 94 % of the cases of group A and in 58 % of group B. A pancreatic primary was predicted in 83 % (group A) and 20 % (group B), respectively. The combined sensitivity of group A and B was 75 % for ileal NETs and 38 % for pancreatic NETs. Immunohistochemical analysis of group C revealed an overall sensitivity of 80 %. Gene and protein expression analysis of CD302 and PPWD1 in NET metastases correctly identifies the primary in the pancreas or the ileum in 80 % of the cases, provided that the tissue is well preserved. Immunohistochemical profiling revealed CD302 as the best marker for ileal and PPWD1 for pancreatic detection.",
keywords = "Endocrine Glands, Female, Gene Expression Profiling, Humans, Male, Neoplasm Metastasis, Neoplasms",
author = "D Kaemmerer and N Posorski and {von Eggeling}, F and G Ernst and D H{\"o}rsch and Baum, {R P} and V Prasad and R Langer and I Esposito and G Kl{\"o}ppel and S Sehner and T Kn{\"o}sel and M Hommann",
year = "2014",
doi = "10.1007/s10585-014-9672-3",
language = "English",
volume = "31",
pages = "817--27",
journal = "CLIN EXP METASTAS",
issn = "0262-0898",
publisher = "Springer Netherlands",
number = "7",

}

RIS

TY - JOUR

T1 - The search for the primary tumor in metastasized gastroenteropancreatic neuroendocrine neoplasm

AU - Kaemmerer, D

AU - Posorski, N

AU - von Eggeling, F

AU - Ernst, G

AU - Hörsch, D

AU - Baum, R P

AU - Prasad, V

AU - Langer, R

AU - Esposito, I

AU - Klöppel, G

AU - Sehner, S

AU - Knösel, T

AU - Hommann, M

PY - 2014

Y1 - 2014

N2 - Gastroenteropancreatic neuroendocrine tumors (NETs) often present as liver metastasis from a carcinoma of unknown primary. We recently showed that primary NETs from the pancreas, small intestine and stomach as well as their respective liver metastases differ from each other by the expression profile of the three genes CD302, PPWD1 and ABHB14B. The gene and protein expression of CD302, PPWD1, and ABHB14B was studied in abdominal NET metastases to identify the site of the respective primary tumors. Cryopreserved tissue from NET metastases collected in different institutions (group A: 29, group B: 50, group C: 132 specimens) were examined by comparative genomic hybridization (Agilent 105 K), gene expression analysis (Agilent 44 K) (groups A and B) and immunohistochemistry (group C). The data were blindly evaluated, i.e. without knowing the site of the primary. Gene expression analysis correctly revealed the primary in the ileum in 94 % of the cases of group A and in 58 % of group B. A pancreatic primary was predicted in 83 % (group A) and 20 % (group B), respectively. The combined sensitivity of group A and B was 75 % for ileal NETs and 38 % for pancreatic NETs. Immunohistochemical analysis of group C revealed an overall sensitivity of 80 %. Gene and protein expression analysis of CD302 and PPWD1 in NET metastases correctly identifies the primary in the pancreas or the ileum in 80 % of the cases, provided that the tissue is well preserved. Immunohistochemical profiling revealed CD302 as the best marker for ileal and PPWD1 for pancreatic detection.

AB - Gastroenteropancreatic neuroendocrine tumors (NETs) often present as liver metastasis from a carcinoma of unknown primary. We recently showed that primary NETs from the pancreas, small intestine and stomach as well as their respective liver metastases differ from each other by the expression profile of the three genes CD302, PPWD1 and ABHB14B. The gene and protein expression of CD302, PPWD1, and ABHB14B was studied in abdominal NET metastases to identify the site of the respective primary tumors. Cryopreserved tissue from NET metastases collected in different institutions (group A: 29, group B: 50, group C: 132 specimens) were examined by comparative genomic hybridization (Agilent 105 K), gene expression analysis (Agilent 44 K) (groups A and B) and immunohistochemistry (group C). The data were blindly evaluated, i.e. without knowing the site of the primary. Gene expression analysis correctly revealed the primary in the ileum in 94 % of the cases of group A and in 58 % of group B. A pancreatic primary was predicted in 83 % (group A) and 20 % (group B), respectively. The combined sensitivity of group A and B was 75 % for ileal NETs and 38 % for pancreatic NETs. Immunohistochemical analysis of group C revealed an overall sensitivity of 80 %. Gene and protein expression analysis of CD302 and PPWD1 in NET metastases correctly identifies the primary in the pancreas or the ileum in 80 % of the cases, provided that the tissue is well preserved. Immunohistochemical profiling revealed CD302 as the best marker for ileal and PPWD1 for pancreatic detection.

KW - Endocrine Glands

KW - Female

KW - Gene Expression Profiling

KW - Humans

KW - Male

KW - Neoplasm Metastasis

KW - Neoplasms

U2 - 10.1007/s10585-014-9672-3

DO - 10.1007/s10585-014-9672-3

M3 - SCORING: Journal article

C2 - 25098566

VL - 31

SP - 817

EP - 827

JO - CLIN EXP METASTAS

JF - CLIN EXP METASTAS

SN - 0262-0898

IS - 7

ER -