The rostral migratory stream generates hippocampal CA1 pyramidal-like neurons in a novel organotypic slice co-culture model

Ilyas Singec; Rolf Knoth; Imre Vida; Michael Frotscher

doi:10.1242/bio.012096

The rostral migratory stream generates hippocampal CA1 pyramidal-like neurons in a novel organotypic slice co-culture model

Standard

The rostral migratory stream generates hippocampal CA1 pyramidal-like neurons in a novel organotypic slice co-culture model. / Singec, Ilyas; Knoth, Rolf; Vida, Imre; Frotscher, Michael.

In: BIOL OPEN, Vol. 4, No. 10, 2015, p. 1222-8.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Singec, I, Knoth, R, Vida, I & Frotscher, M 2015, 'The rostral migratory stream generates hippocampal CA1 pyramidal-like neurons in a novel organotypic slice co-culture model', BIOL OPEN, vol. 4, no. 10, pp. 1222-8. https://doi.org/10.1242/bio.012096

APA

Singec, I., Knoth, R., Vida, I., & Frotscher, M. (2015). The rostral migratory stream generates hippocampal CA1 pyramidal-like neurons in a novel organotypic slice co-culture model. BIOL OPEN, 4(10), 1222-8. https://doi.org/10.1242/bio.012096

Vancouver

Singec I, Knoth R, Vida I, Frotscher M. The rostral migratory stream generates hippocampal CA1 pyramidal-like neurons in a novel organotypic slice co-culture model. BIOL OPEN. 2015;4(10):1222-8. https://doi.org/10.1242/bio.012096

Bibtex

@article{f29e8dff4242407d86fd035103aea5fd,

title = "The rostral migratory stream generates hippocampal CA1 pyramidal-like neurons in a novel organotypic slice co-culture model",

abstract = "The mouse subventricular zone (SVZ) generates large numbers of neuroblasts, which migrate in a distinct pathway, the rostral migratory stream (RMS), and replace specific interneurons in the olfactory bulb (OB). Here, we introduce an organotypic slice culture model that directly connects the RMS to the hippocampus as a new destination. RMS neuroblasts widely populate the hippocampus and undergo cellular differentiation. We demonstrate that RMS cells give rise to various neuronal subtypes and, surprisingly, to CA1 pyramidal neurons. Pyramidal neurons are typically generated before birth and are lost in various neurological disorders. Hence, this unique slice culture model enables us to investigate their postnatal genesis under defined in vitro conditions from the RMS, an unanticipated source for hippocampal pyramidal neurons.",

author = "Ilyas Singec and Rolf Knoth and Imre Vida and Michael Frotscher",

note = "{\textcopyright} 2015. Published by The Company of Biologists Ltd.",

year = "2015",

doi = "10.1242/bio.012096",

language = "English",

volume = "4",

pages = "1222--8",

journal = "BIOL OPEN",

issn = "2046-6390",

publisher = "Company of Biologists Ltd",

number = "10",

}

RIS

TY - JOUR

T1 - The rostral migratory stream generates hippocampal CA1 pyramidal-like neurons in a novel organotypic slice co-culture model

AU - Singec, Ilyas

AU - Knoth, Rolf

AU - Vida, Imre

AU - Frotscher, Michael

PY - 2015

Y1 - 2015

N2 - The mouse subventricular zone (SVZ) generates large numbers of neuroblasts, which migrate in a distinct pathway, the rostral migratory stream (RMS), and replace specific interneurons in the olfactory bulb (OB). Here, we introduce an organotypic slice culture model that directly connects the RMS to the hippocampus as a new destination. RMS neuroblasts widely populate the hippocampus and undergo cellular differentiation. We demonstrate that RMS cells give rise to various neuronal subtypes and, surprisingly, to CA1 pyramidal neurons. Pyramidal neurons are typically generated before birth and are lost in various neurological disorders. Hence, this unique slice culture model enables us to investigate their postnatal genesis under defined in vitro conditions from the RMS, an unanticipated source for hippocampal pyramidal neurons.

AB - The mouse subventricular zone (SVZ) generates large numbers of neuroblasts, which migrate in a distinct pathway, the rostral migratory stream (RMS), and replace specific interneurons in the olfactory bulb (OB). Here, we introduce an organotypic slice culture model that directly connects the RMS to the hippocampus as a new destination. RMS neuroblasts widely populate the hippocampus and undergo cellular differentiation. We demonstrate that RMS cells give rise to various neuronal subtypes and, surprisingly, to CA1 pyramidal neurons. Pyramidal neurons are typically generated before birth and are lost in various neurological disorders. Hence, this unique slice culture model enables us to investigate their postnatal genesis under defined in vitro conditions from the RMS, an unanticipated source for hippocampal pyramidal neurons.

U2 - 10.1242/bio.012096

DO - 10.1242/bio.012096

M3 - SCORING: Journal article

C2 - 26340944

VL - 4

SP - 1222

EP - 1228

JO - BIOL OPEN

JF - BIOL OPEN

SN - 2046-6390

IS - 10

ER -