The role of single cell derived vascular resident endothelial progenitor cells in the enhancement of vascularization in scaffold-based skin regeneration
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The role of single cell derived vascular resident endothelial progenitor cells in the enhancement of vascularization in scaffold-based skin regeneration. / Zhang, Ziyang; Ito, Wulf D; Hopfner, Ursula; Böhmert, Björn; Kremer, Mathias; Reckhenrich, Ann K; Harder, Yves; Lund, Natalie; Kruse, Charli; Machens, Hans-Günther; Egaña, José T.
In: BIOMATERIALS, Vol. 32, No. 17, 06.2011, p. 4109-4117.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - The role of single cell derived vascular resident endothelial progenitor cells in the enhancement of vascularization in scaffold-based skin regeneration
AU - Zhang, Ziyang
AU - Ito, Wulf D
AU - Hopfner, Ursula
AU - Böhmert, Björn
AU - Kremer, Mathias
AU - Reckhenrich, Ann K
AU - Harder, Yves
AU - Lund, Natalie
AU - Kruse, Charli
AU - Machens, Hans-Günther
AU - Egaña, José T
N1 - Copyright © 2011 Elsevier Ltd. All rights reserved.
PY - 2011/6
Y1 - 2011/6
N2 - Increasing evidence suggests that vascular resident endothelial progenitor cells (VR-EPCs) are present in several organs, playing an important role in postnatal neovascularization. Here, we isolated and characterized VR-EPCs from cardiac tissue in vitro, evaluating their regenerative potential in vivo. VR-EPCs showed to be highly clonogenic and expressed several stem and differentiation markers. Under endothelial differentiation conditions, cells form capillary-like structures, in contrast to osteogenic or adipogenic differentiation conditions where no functional changes were observed. After seeding in scaffolds, cells were distributed homogeneously and directly attached to the scaffold. Then, cell seeded scaffolds were used to induce dermal regeneration in a nude mice full skin defect model. The presence of VR-EPCs enhanced dermal vascularization. Histological assays showed increased vessel number (p < 0.05) and cellularization (p < 0.05) in VR-EPCs group. In order to explore possible mechanisms of vascular regeneration, in vitro experiments were performed. Results showed that pro-angiogenic environments increased the migration capacity (p < 0.001) and ability to form capillary-like structures (p < 0.05) of VR-EPC. In addition, VR-EPCs secreted several pro-angiogenic molecules including VEGF and PDGF. These results indicate that a highly clonogenic population of VR-EPCs might be established in vitro, representing a new source for therapeutic vascularization in tissue engineering and regeneration.
AB - Increasing evidence suggests that vascular resident endothelial progenitor cells (VR-EPCs) are present in several organs, playing an important role in postnatal neovascularization. Here, we isolated and characterized VR-EPCs from cardiac tissue in vitro, evaluating their regenerative potential in vivo. VR-EPCs showed to be highly clonogenic and expressed several stem and differentiation markers. Under endothelial differentiation conditions, cells form capillary-like structures, in contrast to osteogenic or adipogenic differentiation conditions where no functional changes were observed. After seeding in scaffolds, cells were distributed homogeneously and directly attached to the scaffold. Then, cell seeded scaffolds were used to induce dermal regeneration in a nude mice full skin defect model. The presence of VR-EPCs enhanced dermal vascularization. Histological assays showed increased vessel number (p < 0.05) and cellularization (p < 0.05) in VR-EPCs group. In order to explore possible mechanisms of vascular regeneration, in vitro experiments were performed. Results showed that pro-angiogenic environments increased the migration capacity (p < 0.001) and ability to form capillary-like structures (p < 0.05) of VR-EPC. In addition, VR-EPCs secreted several pro-angiogenic molecules including VEGF and PDGF. These results indicate that a highly clonogenic population of VR-EPCs might be established in vitro, representing a new source for therapeutic vascularization in tissue engineering and regeneration.
KW - Animals
KW - Blood Vessels/growth & development
KW - Cell Differentiation
KW - Cell Migration Assays
KW - Dermis/pathology
KW - Endothelial Cells/cytology
KW - Guided Tissue Regeneration
KW - Mice
KW - Mice, Nude
KW - Models, Animal
KW - Myocardium/cytology
KW - Neovascularization, Physiologic
KW - Rats
KW - Stem Cell Transplantation
KW - Tissue Engineering
KW - Tissue Scaffolds
U2 - 10.1016/j.biomaterials.2011.02.036
DO - 10.1016/j.biomaterials.2011.02.036
M3 - SCORING: Journal article
C2 - 21435711
VL - 32
SP - 4109
EP - 4117
JO - BIOMATERIALS
JF - BIOMATERIALS
SN - 0142-9612
IS - 17
ER -
