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The RNA-binding protein MARTA2 regulates dendritic targeting of MAP2 mRNAs in rat neurons

Standard

The RNA-binding protein MARTA2 regulates dendritic targeting of MAP2 mRNAs in rat neurons. / Zivraj, Krishna H; Rehbein, Monika; Ölschläger-Schütt, Janin; Schob, Claudia; Falley, Katrin; Buck, Friedrich; Schweizer, Michaela; Schepis, Antonino; Kremmer, Elisabeth; Richter, Dietmar; Kreienkamp, Hans-Jürgen; Kindler, Stefan.

In: J NEUROCHEM, Vol. 124, No. 5, 01.03.2013, p. 670-84.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Zivraj, KH, Rehbein, M, Ölschläger-Schütt, J, Schob, C, Falley, K, Buck, F, Schweizer, M, Schepis, A, Kremmer, E, Richter, D, Kreienkamp, H-J & Kindler, S 2013, 'The RNA-binding protein MARTA2 regulates dendritic targeting of MAP2 mRNAs in rat neurons', J NEUROCHEM, vol. 124, no. 5, pp. 670-84. https://doi.org/10.1111/jnc.12079

APA

Zivraj, K. H., Rehbein, M., Ölschläger-Schütt, J., Schob, C., Falley, K., Buck, F., Schweizer, M., Schepis, A., Kremmer, E., Richter, D., Kreienkamp, H-J., & Kindler, S. (2013). The RNA-binding protein MARTA2 regulates dendritic targeting of MAP2 mRNAs in rat neurons. J NEUROCHEM, 124(5), 670-84. https://doi.org/10.1111/jnc.12079

Vancouver

Zivraj KH, Rehbein M, Ölschläger-Schütt J, Schob C, Falley K, Buck F et al. The RNA-binding protein MARTA2 regulates dendritic targeting of MAP2 mRNAs in rat neurons. J NEUROCHEM. 2013 Mar 1;124(5):670-84. https://doi.org/10.1111/jnc.12079

Bibtex

@article{51b3ea11116c4fac9dc66ef4b35d423d,
title = "The RNA-binding protein MARTA2 regulates dendritic targeting of MAP2 mRNAs in rat neurons",
abstract = "Dendritic targeting of mRNAs encoding the microtubule-associated protein 2 (MAP2) in neurons involves a cis-acting dendritic targeting element. Two rat brain proteins, MAP2-RNA trans-acting protein (MARTA)1 and MARTA2, bind to the cis-element with both high affinity and specificity. In this study, affinity-purified MARTA2 was identified as orthologue of human far-upstream element binding protein 3. In neurons, it resides in somatodendritic granules and dendritic spines and associates with MAP2 mRNAs. Expression of a dominant-negative variant of MARTA2 disrupts dendritic targeting of endogenous MAP2 mRNAs, while not noticeably altering the level and subcellular distribution of polyadenylated mRNAs as a whole. Finally, MAP2 transcripts associate with the microtubule-based motor KIF5 and inhibition of KIF5, but not cytoplasmic dynein function disrupts extrasomatic trafficking of MAP2 mRNA granules. Thus, in neurons MARTA2 appears to represent a key trans-acting factor involved in KIF5-mediated dendritic targeting of MAP2 mRNAs.",
keywords = "Animals, Blotting, Western, Dendrites, Immunohistochemistry, Immunoprecipitation, In Situ Hybridization, Mass Spectrometry, Microscopy, Immunoelectron, Microtubule-Associated Proteins, Neurons, Protein Transport, RNA, Messenger, RNA-Binding Proteins, Rats, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction",
author = "Zivraj, {Krishna H} and Monika Rehbein and Janin {\"O}lschl{\"a}ger-Sch{\"u}tt and Claudia Schob and Katrin Falley and Friedrich Buck and Michaela Schweizer and Antonino Schepis and Elisabeth Kremmer and Dietmar Richter and Hans-J{\"u}rgen Kreienkamp and Stefan Kindler",
note = "{\textcopyright} 2012 International Society for Neurochemistry.",
year = "2013",
month = mar,
day = "1",
doi = "10.1111/jnc.12079",
language = "English",
volume = "124",
pages = "670--84",
journal = "J NEUROCHEM",
issn = "0022-3042",
publisher = "Wiley-Blackwell",
number = "5",

}

RIS

TY - JOUR

T1 - The RNA-binding protein MARTA2 regulates dendritic targeting of MAP2 mRNAs in rat neurons

AU - Zivraj, Krishna H

AU - Rehbein, Monika

AU - Ölschläger-Schütt, Janin

AU - Schob, Claudia

AU - Falley, Katrin

AU - Buck, Friedrich

AU - Schweizer, Michaela

AU - Schepis, Antonino

AU - Kremmer, Elisabeth

AU - Richter, Dietmar

AU - Kreienkamp, Hans-Jürgen

AU - Kindler, Stefan

N1 - © 2012 International Society for Neurochemistry.

PY - 2013/3/1

Y1 - 2013/3/1

N2 - Dendritic targeting of mRNAs encoding the microtubule-associated protein 2 (MAP2) in neurons involves a cis-acting dendritic targeting element. Two rat brain proteins, MAP2-RNA trans-acting protein (MARTA)1 and MARTA2, bind to the cis-element with both high affinity and specificity. In this study, affinity-purified MARTA2 was identified as orthologue of human far-upstream element binding protein 3. In neurons, it resides in somatodendritic granules and dendritic spines and associates with MAP2 mRNAs. Expression of a dominant-negative variant of MARTA2 disrupts dendritic targeting of endogenous MAP2 mRNAs, while not noticeably altering the level and subcellular distribution of polyadenylated mRNAs as a whole. Finally, MAP2 transcripts associate with the microtubule-based motor KIF5 and inhibition of KIF5, but not cytoplasmic dynein function disrupts extrasomatic trafficking of MAP2 mRNA granules. Thus, in neurons MARTA2 appears to represent a key trans-acting factor involved in KIF5-mediated dendritic targeting of MAP2 mRNAs.

AB - Dendritic targeting of mRNAs encoding the microtubule-associated protein 2 (MAP2) in neurons involves a cis-acting dendritic targeting element. Two rat brain proteins, MAP2-RNA trans-acting protein (MARTA)1 and MARTA2, bind to the cis-element with both high affinity and specificity. In this study, affinity-purified MARTA2 was identified as orthologue of human far-upstream element binding protein 3. In neurons, it resides in somatodendritic granules and dendritic spines and associates with MAP2 mRNAs. Expression of a dominant-negative variant of MARTA2 disrupts dendritic targeting of endogenous MAP2 mRNAs, while not noticeably altering the level and subcellular distribution of polyadenylated mRNAs as a whole. Finally, MAP2 transcripts associate with the microtubule-based motor KIF5 and inhibition of KIF5, but not cytoplasmic dynein function disrupts extrasomatic trafficking of MAP2 mRNA granules. Thus, in neurons MARTA2 appears to represent a key trans-acting factor involved in KIF5-mediated dendritic targeting of MAP2 mRNAs.

KW - Animals

KW - Blotting, Western

KW - Dendrites

KW - Immunohistochemistry

KW - Immunoprecipitation

KW - In Situ Hybridization

KW - Mass Spectrometry

KW - Microscopy, Immunoelectron

KW - Microtubule-Associated Proteins

KW - Neurons

KW - Protein Transport

KW - RNA, Messenger

KW - RNA-Binding Proteins

KW - Rats

KW - Real-Time Polymerase Chain Reaction

KW - Reverse Transcriptase Polymerase Chain Reaction

U2 - 10.1111/jnc.12079

DO - 10.1111/jnc.12079

M3 - SCORING: Journal article

C2 - 23121659

VL - 124

SP - 670

EP - 684

JO - J NEUROCHEM

JF - J NEUROCHEM

SN - 0022-3042

IS - 5

ER -