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The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signaling

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The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signaling. / Scheib, Ulrike; Stehfest, Katja; Gee, Christine E; Körschen, Heinz G; Fudim, Roman; Oertner, Thomas G; Hegemann, Peter.

In: SCI SIGNAL, Vol. 8, No. 389, 2015, p. rs8.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Scheib, U, Stehfest, K, Gee, CE, Körschen, HG, Fudim, R, Oertner, TG & Hegemann, P 2015, 'The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signaling', SCI SIGNAL, vol. 8, no. 389, pp. rs8. https://doi.org/10.1126/scisignal.aab0611

APA

Scheib, U., Stehfest, K., Gee, C. E., Körschen, H. G., Fudim, R., Oertner, T. G., & Hegemann, P. (2015). The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signaling. SCI SIGNAL, 8(389), rs8. https://doi.org/10.1126/scisignal.aab0611

Vancouver

Scheib U, Stehfest K, Gee CE, Körschen HG, Fudim R, Oertner TG et al. The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signaling. SCI SIGNAL. 2015;8(389):rs8. https://doi.org/10.1126/scisignal.aab0611

Bibtex

@article{7cc3436a7dda45c9bed34ef9cf1302ce,
title = "The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signaling",
abstract = "Blastocladiomycota fungi form motile zoospores that are guided by sensory photoreceptors to areas of optimal light conditions. We showed that the microbial rhodopsin of Blastocladiella emersonii is a rhodopsin-guanylyl cyclase (RhGC), a member of a previously uncharacterized rhodopsin class of light-activated enzymes that generate the second messenger cyclic guanosine monophosphate (cGMP). Upon application of a short light flash, recombinant RhGC converted within 8 ms into a signaling state with blue-shifted absorption from which the dark state recovered within 100 ms. When expressed in Xenopus oocytes, Chinese hamster ovary cells, or mammalian neurons, RhGC generated cGMP in response to green light in a light dose-dependent manner on a subsecond time scale. Thus, we propose RhGC as a versatile tool for the optogenetic analysis of cGMP-dependent signaling processes in cell biology and the neurosciences.",
author = "Ulrike Scheib and Katja Stehfest and Gee, {Christine E} and K{\"o}rschen, {Heinz G} and Roman Fudim and Oertner, {Thomas G} and Peter Hegemann",
note = "Christine E Gee is a co-first author on this publication, Thomas G Oertner is co-senior author",
year = "2015",
doi = "10.1126/scisignal.aab0611",
language = "English",
volume = "8",
pages = "rs8",
journal = "SCI SIGNAL",
issn = "1945-0877",
publisher = "American Association for the Advancement of Science",
number = "389",

}

RIS

TY - JOUR

T1 - The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signaling

AU - Scheib, Ulrike

AU - Stehfest, Katja

AU - Gee, Christine E

AU - Körschen, Heinz G

AU - Fudim, Roman

AU - Oertner, Thomas G

AU - Hegemann, Peter

N1 - Christine E Gee is a co-first author on this publication, Thomas G Oertner is co-senior author

PY - 2015

Y1 - 2015

N2 - Blastocladiomycota fungi form motile zoospores that are guided by sensory photoreceptors to areas of optimal light conditions. We showed that the microbial rhodopsin of Blastocladiella emersonii is a rhodopsin-guanylyl cyclase (RhGC), a member of a previously uncharacterized rhodopsin class of light-activated enzymes that generate the second messenger cyclic guanosine monophosphate (cGMP). Upon application of a short light flash, recombinant RhGC converted within 8 ms into a signaling state with blue-shifted absorption from which the dark state recovered within 100 ms. When expressed in Xenopus oocytes, Chinese hamster ovary cells, or mammalian neurons, RhGC generated cGMP in response to green light in a light dose-dependent manner on a subsecond time scale. Thus, we propose RhGC as a versatile tool for the optogenetic analysis of cGMP-dependent signaling processes in cell biology and the neurosciences.

AB - Blastocladiomycota fungi form motile zoospores that are guided by sensory photoreceptors to areas of optimal light conditions. We showed that the microbial rhodopsin of Blastocladiella emersonii is a rhodopsin-guanylyl cyclase (RhGC), a member of a previously uncharacterized rhodopsin class of light-activated enzymes that generate the second messenger cyclic guanosine monophosphate (cGMP). Upon application of a short light flash, recombinant RhGC converted within 8 ms into a signaling state with blue-shifted absorption from which the dark state recovered within 100 ms. When expressed in Xenopus oocytes, Chinese hamster ovary cells, or mammalian neurons, RhGC generated cGMP in response to green light in a light dose-dependent manner on a subsecond time scale. Thus, we propose RhGC as a versatile tool for the optogenetic analysis of cGMP-dependent signaling processes in cell biology and the neurosciences.

U2 - 10.1126/scisignal.aab0611

DO - 10.1126/scisignal.aab0611

M3 - SCORING: Journal article

C2 - 26268609

VL - 8

SP - rs8

JO - SCI SIGNAL

JF - SCI SIGNAL

SN - 1945-0877

IS - 389

ER -