The presence of collagen III and IV in experimental microsurgical anastomoses of arteries

TY - JOUR

T1 - The presence of collagen III and IV in experimental microsurgical anastomoses of arteries

AU - Friedrich, R E

AU - Bartel-Friedrich, S

AU - Plambeck, K

PY - 2000/10/1

Y1 - 2000/10/1

N2 - PURPOSE: This study investigated the distribution of collagen III and IV in experimental microvascular anastomoses and correlated the findings with the quality of the healing process.MATERIALS AND METHODS: A previously excised and reanastomosed section of the left common carotid artery of 21 female Wistar rats were explanted 4 weeks after surgery. Collagens III and IV were identified in perfusion-fixed specimens by immunohistochemistry.RESULTS: The identification and distribution of collagens reflected the quality of each suturing procedure. A myointimal hyperplasia was present, extending into the vascular lumen, and was closely related to the ends of the vessel segments. The extent of myointimal hyperplasia was dependent on the length and the vitality of the vessel segments. The media was the weakest part of the tissue layers with regard to regenerative potential in the axial direction. The marked cuff of the adventitia around the anastomosis was rich in collagen III-positive vasa vasorum. Collagen IV was distributed in net-like patterns in the media. The distribution pattern in the media was not observed in anastomoses with incomplete approximation and necrotic segment ends. The collagen IV-positive subendothelial basal membrane was incomplete in the aneurysms because of partial necrosis.CONCLUSIONS: The experimental findings support the need for atraumatic suturing in microvascular surgery. Immunohistochemical detection of collagens can be a valuable tool for studying the basic processes of wound healing and the integration of microvascular flaps into their recipient sites.

AB - PURPOSE: This study investigated the distribution of collagen III and IV in experimental microvascular anastomoses and correlated the findings with the quality of the healing process.MATERIALS AND METHODS: A previously excised and reanastomosed section of the left common carotid artery of 21 female Wistar rats were explanted 4 weeks after surgery. Collagens III and IV were identified in perfusion-fixed specimens by immunohistochemistry.RESULTS: The identification and distribution of collagens reflected the quality of each suturing procedure. A myointimal hyperplasia was present, extending into the vascular lumen, and was closely related to the ends of the vessel segments. The extent of myointimal hyperplasia was dependent on the length and the vitality of the vessel segments. The media was the weakest part of the tissue layers with regard to regenerative potential in the axial direction. The marked cuff of the adventitia around the anastomosis was rich in collagen III-positive vasa vasorum. Collagen IV was distributed in net-like patterns in the media. The distribution pattern in the media was not observed in anastomoses with incomplete approximation and necrotic segment ends. The collagen IV-positive subendothelial basal membrane was incomplete in the aneurysms because of partial necrosis.CONCLUSIONS: The experimental findings support the need for atraumatic suturing in microvascular surgery. Immunohistochemical detection of collagens can be a valuable tool for studying the basic processes of wound healing and the integration of microvascular flaps into their recipient sites.

KW - Anastomosis, Surgical

KW - Animals

KW - Arteries

KW - Basement Membrane

KW - Carotid Artery, Common

KW - Cicatrix

KW - Collagen

KW - Endothelium, Vascular

KW - Female

KW - Immunohistochemistry

KW - Microcirculation

KW - Microsurgery

KW - Rats

KW - Rats, Wistar

KW - Surgical Flaps

KW - Suture Techniques

KW - Vasa Vasorum

KW - Wound Healing

U2 - 10.1053/joms.2000.9572

DO - 10.1053/joms.2000.9572

M3 - SCORING: Journal article

C2 - 11021707

VL - 58

SP - 1125

EP - 1128

JO - J ORAL MAXIL SURG

JF - J ORAL MAXIL SURG

SN - 0278-2391

IS - 10

ER -